Anti usp9x

Manufactured by Abcam

Anti-USP9x is a primary antibody that specifically targets the USP9x protein. USP9x is a deubiquitinating enzyme that plays a role in the regulation of various cellular processes. This antibody can be used in applications such as Western blotting to detect and analyze the expression of USP9x in biological samples.

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Lab products found in correlation

Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions) Anti smad2 3, by Cell Signaling Technology (1 mentions) Anti β actin hrp, by Merck Group (1 mentions) Anti fibrillarin, by Abcam (1 mentions) Anti p jnk, by Cell Signaling Technology (1 mentions) Anti gapdh hrp, by Abcam (1 mentions) Anti p smad3, by Merck Group (1 mentions) Anti p smad5, by Abcam (1 mentions) Anti smad4, by Abcam (1 mentions) Anti p smad2, by Cell Signaling Technology (1 mentions) Anti ha clone 3f10, by Roche (1 mentions) Anti flag 2368, by Cell Signaling Technology (1 mentions) Anti mcl 1 d35a5, by Cell Signaling Technology (1 mentions) Anti hsp70, by Abcam (1 mentions) Anti usp7, by Abcam (1 mentions) Hrp conjugated secondary antibody, by Abcam (1 mentions)

2 protocols using anti usp9x

Cellular Fractionation and Western Blot Analysis

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Antibodies used for flow cytometry are described in S2 Table. Cells were analyzed on a FACSCanto II flow cytometer using FlowJo software (Tree Star Inc.). Protein from whole cell lysate (total protein), nuclear fraction and cytoplasmic fraction was purified using NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher). Antibodies for Western blotting included: anti-GAPDH-HRP (Abcam), anti-β actin-HRP (Sigma), anti-fibrillarin (Abcam), anti-SMAD4 (Abcam), anti-SMAD2, 3 (Cell Signaling Tech), anti-JNK (Santa Cruz Biotechonolgy), anti p-JNK (Cell Signaling Tech), anti-p-SMAD2 (Cell Signaling Tech), anti-p-SMAD3 (Millipore), anti-p-SMAD5 (Abcam), anti-USP9x (Abcam), and anti-TIF1γ (TRIM33) (Abcam).

Ge J., Apicella M., Mills J.A., Garçon L., French D.L., Weiss M.J., Bessler M, & Mason P.J. (2015). Dysregulation of the Transforming Growth Factor β Pathway in Induced Pluripotent Stem Cells Generated from Patients with Diamond Blackfan Anemia. PLoS ONE, 10(8), e0134878.

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Antibody Panel for Protein Profiling

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The following antibodies were used: anti-K48-linked ubiquitin, clone APU2 and anti-K63-linked ubiquitin, clone APU3 (Millipore); anti-PARP 9542, anti-cAbl 2862, anti-α-tubulin 2156, anti-Mcl-1 D35A5, anti-Flag 2368, (Cell Signaling Technologies); anti-actin clone AC-40 A3853, anti-GAPDH clone G9295, and anti-HA, Clone 3F10 (Roche); anti-ubiquitin, clone 6C1.17 (BD Pharmingen); anti-HSP70, anti-USP7, anti-UCH37, anti-USP24 and anti-USP9x (all rabbit monoclonal) (Abcam); HRP conjugated secondary antibodies (Abcam)

Lawson A.P., Long M.J., Coffey R.T., Qian Y., Weerapana E., El Oualid F, & Hedstrom L. (2015). Naturally occurring isothiocyanates exert anticancer effects by inhibiting deubiquitinating enzymes. Cancer research, 75(23), 5130-5142.

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