Cell lysates were prepared from parental and oralspheres derived from each of Cal27 and OSC19 cell lines. Proteins were separated by SDS-PAGE and transferred onto 0.45 μM nitrocellulose membrane. Membranes were blocked using 5% low fat dry milk in TBST and probed with the following primary antibodies. Proteins were detected using ECL Western Blotting Substrate (Thermo Scientific) and autoradiography. Protein loads were normalized using antibody for GAPDH (Cell Signaling Technologies). The following antibodies were used in this study: Nanog, Notch1, Slug (Cell Signaling Technologies), and c-Fos (Santa Cruz Biotechnology).
Antibody for gapdh
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-GAPDH Antibody is a highly specific antibody that recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a key enzyme involved in the glycolytic pathway and is commonly used as a loading control or reference protein in various biochemical and cell biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Notch1, by Cell Signaling Technology (1 mentions)
Nanog, by Cell Signaling Technology (1 mentions)
C fos, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg hrp, by Cell Signaling Technology (1 mentions)
Anti mouse igg hrp, by Cell Signaling Technology (1 mentions)
Pgc 1α, by Proteintech (1 mentions)
Sirt1, by Proteintech (1 mentions)
Parp1, by Proteintech (1 mentions)
Tnf α, by Affinity Biosciences (1 mentions)
Bcl2 associated x (bax), by Affinity Biosciences (1 mentions)
Caspase 3, by Proteintech (1 mentions)
Bcl 2, by Affinity Biosciences (1 mentions)
2 protocols using antibody for gapdh
1
Protein Expression Profiling of Oral Cancer Spheres
2
Investigating Key Apoptotic Regulators
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The primary antibodies Bax (Cat# AF1020), Bcl-2 (Cat# AF6139), TFAM (Cat# DF3232), and TNF-α (Cat# AF7014) were acquired from Affinity Biosciences (Cincinnati, OH, USA). Antibody for GAPDH (Cat# 2118S) was obtained from Cell Signaling Technology, Inc (Danvers, MA, USA). Antibodies for SIRT1 (Cat# 60303-1-Ig), PGC-1α (Cat# 66369-1-Ig), Nrf2 (Cat# 16396-1-AP), PARP1 (Cat# 13371-1-AP), and Caspase3 (Cat# 19677-1-AP) were purchased from Proteintech Group, Inc (Wuhan, China). Goat anti-rabbit IgG-HRP (Cat# 98164S) and anti-mouse IgG-HRP (Cat# 91196S) were purchased from Cell Signaling Technology, Inc (Danvers, MA, USA) and Proteintech Group, Inc (Wuhan, China), respectively.
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