Immediately after optical recordings, flies were fixed for 10–20 mins in 4% formaldehyde. We used 1:100 mouse anti-Futsch (22c10, Developmental Studies Hybridoma Bank (DSHB), Univ of Iowa), 1:10,000 rabbit anti-VGlut (Daniels et al., 2004 (link)) (gift of Aaron DiAntonio), 1:50 mouse anti-Syntaxin (8C3, DSHB), 1:500 anti-Tubulin (T6074, Sigma), 1:500 rabbit anti-Phospho-Smad3 (EP823Y, AbCam), goat anti-HRP-Cy3 (1:100, Jackson ImmunoResearch Laboratories, West Grove, PA), goat anti-mouse and anti-rabbit Alexa647 (1:1000, Molecular Probes, Eugene, OR) and goat anti-GFP Alexa488 (1:500, Molecular Probes). Antibody stains were imaged on a Zeiss 510 Axioplan confocal or Zeiss 780 AxioExaminer confocal (Oberkochen, Germany) with a 63×/1.4 oil objective. Scale bars = 5 µm throughout paper.
Goat anti hrp cy3
Manufactured by Jackson ImmunoResearch
Sourced in Panama
Goat anti-HRP-Cy3 is a secondary antibody conjugate produced by Jackson ImmunoResearch. It is generated by coupling goat anti-horseradish peroxidase (HRP) antibodies to the fluorescent dye Cy3. This product is designed for use in immunoassays and other applications where detection of HRP-labeled targets is required.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti futsch 22c10, by Developmental Studies Hybridoma Bank (1 mentions)
Anti tubulin t6074, by Merck Group (1 mentions)
Rat anti brdu, by Abcam (1 mentions)
Mouse anti dlg 4f3, by Developmental Studies Hybridoma Bank (1 mentions)
Lsm 700, by Zeiss (1 mentions)
Goat anti rat alexa488, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit alexa633, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit alexa 546, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse alexa 546, by Thermo Fisher Scientific (1 mentions)
Rat anti ha, by Roche (1 mentions)
Rabbit anti dsred, by Takara Bio (1 mentions)
Goat anti chicken alexa 488, by Thermo Fisher Scientific (1 mentions)
Rabbit anti cd4, by Novus Biologicals (1 mentions)
Mouse anti repo, by Developmental Studies Hybridoma Bank (1 mentions)
Chicken anti gfp, by Abcam (1 mentions)
3 protocols using goat anti hrp cy3
1
Immunostaining Drosophila Neuromuscular Junctions
2
Immunohistochemistry of Larval Brains
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Larval brains were dissected and fixed in 4% paraformaldehyde in phosphate-buffered saline for 20 minutes. The primary antibodies used were rabbit anti-Dpn (1:500) from Y.-N. Jan (University of California, San Francisco), mouse anti–Dlg 4F3 (1:20) from the Developmental Studies Hybridoma Bank (DSHB), rat anti-Miranda (1:500) from Chris Doe (University of Oregon), mouse anti-Prospero MR1A (1:20) from DSHB, rat anti-BrdU (1:500) from Abcam, goat anti-HRP-Cy3 from Jackson ImmunoResearch Laboratories, and mouse anti-nc82 (1:5) from DSHB. Secondary antibodies were from Life Technologies. Confocal images were taken with a Nikon D-Eclipse C1 and processed with Image J and Adobe Photoshop.
3
Immunostaining protocol for larval tissues
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The following antibodies were used in this study: chicken anti-GFP (Abcam, 1:500), mouse anti-GRASP (Sigma #G6539, 1:100) 61 , mouse anti-rat CD2 (Bio-Rad, 1:200), rat anti-mCD8 (Caltag, 1:100), rabbit anti-FLRFa (a gift from Dr. Eve Marder, 1:5000) 81 , rabbit anti-CD4 (Novus Biologicals, 1:500), mouse anti-REPO (Developmental Studies Hybridoma Bank 8D12, 1:5), rabbit anti-DsRed (Clontech #632496, 1:200), rat anti-HA (Roche 3F10, 1:100), goat anti-HRP-Cy3 (Jackson ImmunoResearch, 1:100), goat anti-rat Alexa488 (Invitrogen, 1:500), goat anti-chicken Alexa488 (Invitrogen, 1:500), goat anti-mouse Alexa546 (Invitrogen, 1:500), goat anti-rabbit Alexa546 (Invitrogen, 1:500), goat anti-rat Alexa633 (Invitrogen, 1:500), and goat anti-rabbit Alexa633 (Invitrogen, 1:500). Dissected larval tissues were fixed in 4% paraformaldehyde for 30 minutes and then stained as previously described 32 . The images were acquired by using a Zeiss LSM 510 with a 20x/0.75 Plan-Apochromat objective or 40x/1.0 Plan-Apochromat oil immersion objective, Zeiss LSM 700 with a 20x/0.75 Plan-Apochromat objective or 40x/1.0 Plan-Apochromat oil immersion objective, or Leica SP5 with a 40x/0.85 PL APO objective, and digitally processed using Zeiss LSM Image Browser, Leica LAS X Lite, and Adobe Photoshop.
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