Rabbit anti tbp

Manufactured by Cell Signaling Technology

Rabbit anti-TBP is a primary antibody that recognizes the TATA-binding protein (TBP), a core component of the transcription initiation complex. This antibody can be used to detect and study TBP in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Anti-TBP (8 citations)

4 protocols using rabbit anti tbp

Quantitative Western Blot Analysis

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Quantitative Western blot was performed using Odyssey Infrared Imaging System (Licor). Primary antibodies used in this study include rabbit anti-MeCP2 C-terminus (1:4000, in house), mouse anti-MeCP2 N-terminus (1:4000, Sigma M7433), mouse anti-NeuN (1:500, Millipore MAB377), rabbit anti-Avi tag (1:5000, Abcam ab106159, listed as anti-Tavi in main text, detects the minimal peptide substrate of biotin ligase BirA regardless of biotinylation status), rabbit anti-HDAC3 (1:1000, Santa Cruz sc-11417), rabbit anti-TBLR1 (1:1000, Bethyl A300–408A), rabbit anti-Sin3A, (1:500, Thermo Scientific PA1-870), rabbit anti-Histone H3 (1:1000, Abcam ab1791), and rabbit anti-TBP (1:1000, Cell Signaling #8515). Secondary antibodies include anti-rabbit IRDye 680LT (1:10,000, Licor), anti-mouse IRDye 800CW (Licor), Streptavidin Dylight 650 (1:10,000, Fisher 84547) and Streptavidin Dylight 800 (1:10,000, Fisher 21851). Quantification of protein expression levels was carried out following Odyssey Infrared Imaging System protocols. Scans of full-length Western blot membranes are provided in Supplementary Figs. 11–13.

Johnson B.S., Zhao Y.T., Fasolino M., Lamonica J.M., Kim Y.J., Georgakilas G., Wood K.H., Bu D., Cui Y., Goffin D., Vahedi G., Kim T.H, & Zhou Z. (2017). Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome. Nature medicine, 23(10), 1203-1214.

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Western Blot Analysis of Protein Expression

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The separated proteins were electroblotted onto nitrocellulose filter membrane and blocked for 2 h at room temperature with Tris-buffered saline containing 5% BSA. Nitrocellulose filter membrane were incubated at 4 °C overnight with antibodies, rabbit anti-KLF7 (Abcam; ab197690), rabbit anti-IL-6 (Abcam, ab214429), rabbit anti-GPR120 (Abcam; ab230869), rabbit anti-GPR40 (Thermo Fisher; PA5-75351), rabbit anti-p-IKKβ (Cell Signaling Technology; 2697S), rabbit anti-T-IKKβ (Abcam; ab124957), rabbit anti-p-IκB (Cell Signaling Technology; 2859S), rabbit anti-T-IκB (Cell Signaling Technology; 4812S), rabbit anti-p-p65 (Cell Signaling Technology; 3033S), rabbit anti-T-p65 (Cell Signaling Technology; 8242S), rat anti-GAPDH (ZSGB-BIO; TA-08), rabbit anti-TBP (Cell Signaling Technology; 44059S), rat anti-β-Actin (ZSGB-BIO; TA-10) were used at a dilution of 1:1000. The secondary antibodies (ZSGB-BIO; ZB2301 and ZB2305) were incubated at 25 °C for 2 h at a working ratio of 1:10,000.

Qiu T., Yang X., Wang J., Pan C., Chu X., Xiong J., Xie J., Chang Y., Wang C, & Zhang J. (2022). Obesity-induced elevated palmitic acid promotes inflammation and glucose metabolism disorders through GPRs/NF-κB/KLF7 pathway. Nutrition & Diabetes, 12, 23.

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Quantitative Western Blot Analysis

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Western Blot Analysis of Iron Metabolism Proteins

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Protein extracts were prepared with cytoplasmic lysis buffer (25mM Tris-HCl [pH7.4], 40mM KCl, and 1% Triton X-100) supplemented with 1 mg/ml aprotinin and 1 mg/ml leupeptin (all obtained from Sigma). Twenty micrograms of total protein was run on 10%−15% SDS-polyacrylamide gels, and Western blotting was performed exactly as described (Theurl et al., 2006 (link)) with a mouse anti-human TfR1 antibody (1:1000; Invitrogen), rabbit anti-human Ferritin (1:500; Sigma), rabbit anti-Fpn (1:400; self designed; Eurogentic), rabbit anti-HO-1 (1:1000; Enzo), rabbit anti-iNOS (1:1000; BD), rabbit anti-NFκB p65 or rabbit anti-phosphor NFκB p65 (both 1:1000; Cell Signalling). Blotting with either rabbit anti-TBP (1:1000; Cell Signalling) or rabbit anti-Actin antibody (1:1000; Sigma-Aldrich) was performed as a loading control.

Mitterstiller A.M., Haschka D., Dichtl S., Nairz M., Demetz E., Talasz H., Soares M.P., Einwallner E., Esterbauer H., Fang F.C., Geley S, & Weiss G. (2016). Heme oxygenase 1 controls early innate immune response of macrophages to Salmonella Typhimurium infection. Cellular microbiology, 18(10), 1374-1389.

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