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Cy3 and cy5 conjugated secondary antibodies

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Cy3- and Cy5-conjugated secondary antibodies are fluorescently labeled antibodies that bind to the Fc region of primary antibodies. These conjugated secondary antibodies are commonly used in various immunoassays and imaging techniques to detect and visualize target proteins or molecules.

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Lab products found in correlation

Anti ki67, by Thermo Fisher Scientific (2 mentions) Deadend fluorometric tunel system, by Promega (2 mentions) Anti chromogranin a, by Santa Cruz Biotechnology (2 mentions) Anti fabp1, by Novus Biologicals (2 mentions) Anti cd166, by R&D Systems (2 mentions) Fluoview fv1000 spinning disc confocal scan head, by Olympus (2 mentions) Axio imager z1, by Leica (2 mentions) Fv 10 asw 1, by Olympus (2 mentions) Alexa fluor 594 conjugated phalloidin, by Thermo Fisher Scientific (2 mentions) Anti cd44, by BD (2 mentions) Apotome, by Leica (2 mentions) Anti cyclin d1, by Abcam (2 mentions) Ix81 inverted microscope, by Olympus (2 mentions) Anti muc2, by Santa Cruz Biotechnology (2 mentions) Anti lysozyme, by Agilent Technologies (2 mentions)

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Cy3-conjugated antibodies (2 citations)

2 protocols using cy3 and cy5 conjugated secondary antibodies

1

Intestinal Cell Lineage Analysis Through Immunohistochemistry

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Intestinal tissue was harvested and fixed in 4% paraformaldehyde. 8 μm OCT frozen sections or 5 μm paraffin-embedded sections were TUNEL stained using the DeadEnd Fluorometric TUNEL system was used per manufacturer’s instructions (Promega) or immunostained using the following primary antibodies: anti-Ki67 (ThermoFisher #RM-9106), anti-Muc2 (Santa Cruz #sc-15334), anti-lysozyme (Dako #A0099), anti-chromogranin A (Santa Cruz #sc-1488), anti-FABP1 (Novus #NBP1-87695), anti-CD44 (BD Pharmingen #550538), anti-cyclin D1 (Abcam #ab134175) and anti-CD166 (R&D #AF1172). All primary antibodies were used at 1:100 to 1:200. Cy3- and Cy5-conjugated secondary antibodies (Santa Cruz and Jackson ImmunoResearch) were used at 1:500 to 1:1000 dilutions. Alexa Fluor 594-conjugated Phalloidin (Invitrogen) was used at 1:500. CD166 immunostained tissue sections34 were analyzed and confocal images acquired as 0.5-μm planes using an IX81 Inverted Microscope equipped with Fluoview FV1000-Spinning Disc Confocal scan head and FV10 ASW 1.7 software (Olympus). All other images were captured on a Zeiss Axio-Imager Z1 with ApoTome or Leica SP5 confocal microscope.
Yan K.S., Janda C.Y., Chang J., Zheng G.X., Larkin K.A., Luca V.C., Chia L.A., Mah A.T., Han A., Terry J.M., Ootani A., Roelf K., Lee M., Yuan J., Li X., Bolen C.R., Wilhelmy J., Davies P.S., Ueno H., von Furstenberg R.J., Belgrader P., Ziraldo S.B., Ordonez H., Henning S.J., Wong M.H., Snyder M.P., Weissman I.L., Hsueh A.J., Mikkelsen T.S., Garcia K.C, & Kuo C.J. (2017). Non-equivalence of Wnt and R-spondin ligands during Lgr5+ intestinal stem cell self-renewal. Nature, 545(7653), 238-242.
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2

Intestinal Cell Lineage Analysis Through Immunohistochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intestinal tissue was harvested and fixed in 4% paraformaldehyde. 8 μm OCT frozen sections or 5 μm paraffin-embedded sections were TUNEL stained using the DeadEnd Fluorometric TUNEL system was used per manufacturer’s instructions (Promega) or immunostained using the following primary antibodies: anti-Ki67 (ThermoFisher #RM-9106), anti-Muc2 (Santa Cruz #sc-15334), anti-lysozyme (Dako #A0099), anti-chromogranin A (Santa Cruz #sc-1488), anti-FABP1 (Novus #NBP1-87695), anti-CD44 (BD Pharmingen #550538), anti-cyclin D1 (Abcam #ab134175) and anti-CD166 (R&D #AF1172). All primary antibodies were used at 1:100 to 1:200. Cy3- and Cy5-conjugated secondary antibodies (Santa Cruz and Jackson ImmunoResearch) were used at 1:500 to 1:1000 dilutions. Alexa Fluor 594-conjugated Phalloidin (Invitrogen) was used at 1:500. CD166 immunostained tissue sections34 were analyzed and confocal images acquired as 0.5-μm planes using an IX81 Inverted Microscope equipped with Fluoview FV1000-Spinning Disc Confocal scan head and FV10 ASW 1.7 software (Olympus). All other images were captured on a Zeiss Axio-Imager Z1 with ApoTome or Leica SP5 confocal microscope.
Yan K.S., Janda C.Y., Chang J., Zheng G.X., Larkin K.A., Luca V.C., Chia L.A., Mah A.T., Han A., Terry J.M., Ootani A., Roelf K., Lee M., Yuan J., Li X., Bolen C.R., Wilhelmy J., Davies P.S., Ueno H., von Furstenberg R.J., Belgrader P., Ziraldo S.B., Ordonez H., Henning S.J., Wong M.H., Snyder M.P., Weissman I.L., Hsueh A.J., Mikkelsen T.S., Garcia K.C, & Kuo C.J. (2017). Non-equivalence of Wnt and R-spondin ligands during Lgr5+ intestinal stem cell self-renewal. Nature, 545(7653), 238-242.
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