Nextseq 500 550 150 cycle high output kit v2

Manufactured by Illumina

The NextSeq 500/550 150 cycle High Output Kit v2.5 is a lab equipment product designed for use with Illumina's NextSeq 500 and NextSeq 550 sequencing systems. The kit provides the necessary reagents and consumables to perform up to 150 sequencing cycles on these platforms.

Automatically generated - may contain errors

Lab products found in correlation

Nextseq 500, by Illumina (2 mentions) Chromium next gem single cell 3 reagent kit, by 10x Genomics (2 mentions)

Spelling variants of this product

NextSeq 500 (5724 citations) NextSeq (1155 citations) NextSeq 550 (932 citations) NextSeq 500/550 (98 citations) NextSeq 500/550 High Output Kit v2.5 (150 Cycles) (15 citations) NextSeq 500/550 High Output Kit v2 (150 cycles) (9 citations) V2 kits (4 citations) Kit v2 (2 citations) NextSeq with the NextSeq 500/550 High Output Kit v2 (150 cycles) (2 citations)

2 protocols using nextseq 500 550 150 cycle high output kit v2

Single-cell RNA-seq of Murine γδ T Cells

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Single cell suspensions were performed using LNs and spleens from C57BL/6 mice (6–8 weeks old). γδ T cells from pooled LNs and spleens were sorted and were subjected to scRNAseq analysis. Single cells were captured and barcoded cDNA libraries were constructed using the Chromium Next GEM Single Cell 3ʹ Reagent Kit (v3.1, 10X Genomics) and the Chromium Controller, according to manufacturer’s instructions. Libraries were pooled and sequenced using a 28bp x 8bp x 125bp configuration for read1 x i7 index x read2 on the Illumina NextSeq 500 with the NextSeq 500/550 150 cycle High Output Kit v2.5 (20024907).

Wang Y., Qin H., Cai Y., Chen X., Li H., Montoya-Durango D.E., Ding C., Hu X., Chariker J.H., Sarojini H., Chien S., Rouchka E.C., Zhang H.G., Zheng J., Qiu F, & Yan J. (2023). Natural γδT17 cell development and functional acquisition is governed by the mTORC2-c-Maf-controlled mitochondrial fission pathway. iScience, 26(5), 106630.

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Single-Cell RNA Sequencing of Mouse Pancreatic Cells

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Live CD45 + cells were sorted from mouse pancreata, washed, and resuspended in 1x PBS (calcium and magnesium-free) containing 0.04% BSA. Single cells were captured and barcoded cDNA libraries were constructed using the Chromium Next GEM Single-Cell 3ʹ Reagent Kit (v3.1, 10X Genomics) and the Chromium Controller, according to the manufacturer’s instructions. Libraries were pooled and sequenced using a 28 bp × 8 bp × 125 bp configuration for read1 × i7 index x read2 on the Illumina NextSeq 500 with the NextSeq 500/550 150 cycle High Output Kit v2.5 (20024907).

Geller A.E., Shrestha R., Woeste M.R., Guo H., Hu X., Ding C., Andreeva K., Chariker J.H., Zhou M., Tieri D., Watson C.T., Mitchell R.A., Zhang H.G., Li Y., Martin II R.C., Rouchka E.C, & Yan J. (2022). The induction of peripheral trained immunity in the pancreas incites anti-tumor activity to control pancreatic cancer progression. Nature Communications, 13, 759.

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