Rp 8 br cgmps

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rp-8-Br-cGMPS is a cGMP analogue produced by Santa Cruz Biotechnology. It is a cyclic guanosine monophosphate (cGMP) derivative with a bromine substitution at the 8-position of the guanine ring. The core function of Rp-8-Br-cGMPS is to serve as a research tool in the study of cGMP-dependent signaling pathways.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Triphenyl tetrazolium chloride (ttc), by Merck Group (2 mentions) Rpmi 1640 modified medium, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Kt5823, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) U46619, by Cayman Chemical (1 mentions)

3 protocols using rp 8 br cgmps

Cone Degeneration Protection using PKG Inhibitors

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Two PKG inhibitors, KT5823 (Sigma Aldrich, St Louis, MO) and (R_p)-8-Br-cGMPS (Santa Cruz Biotechnology, Dallas, TX), were used. (R_p)-8-Br-cGMPS is a cyclic nucleotide analog (an Rp-diastereomer of cGMP), whereas KT5823 is a K-series inhibitor that inactivates the ATP-binding site through competition with ATP (44 (link)). These two compounds have been shown to reduce PKG activity in CNG channel-deficient mice and reduce ER stress/cone death (21 (link)). Starting at postnatal day 7 (P7), Cnga3^−/−/Nrl^−/− mice received intraperitoneal injection of KT5823 (1.0 μmol/kg body weight/day), (R_p)-8-Br-cGMPS (5.0 μmol/kg body weight/day), or vehicle for 9 days, as described previously (21 (link)). At the end of the treatment, retinas were collected for biochemical evaluations.

Yang F., Ma H., Butler M.R, & Ding X.Q. (2020). Potential Contribution of Ryanodine Receptor 2 Upregulation to cGMP/PKG Signaling-induced Cone Degeneration in Cyclic Nucleotide-gated Channel Deficiency. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(5), 6335-6350.

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Evaluation of SCU Effects on Cells

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Powdered SCU (purity 99%, formula weight 464.4) was obtained from Mr. Renwei Zhang of Kunming Longjin Pharmaceuticals Co. (Kunming, China). SCU stock solutions (maximum concentration 100 mM) were prepared by dissolving the SCU in physiological saline solution. Cell culture reagents including modified RPMI–1640 medium and fetal bovine serum were obtained from HyClone (Thermo Fisher Scientific, Waltham, MA, USA). PKG inhibitor Rp-8-Br-cGMPS was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). PKG activator 8-pCPT-cGMP [8-(4-Chlorophenylthio) guanosine 3',5'-cyclic monophosphate sodium salt], triphenyl tetrazolium chloride (TTC) and ACH were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Li L., Li L., Chen C., Yang J., Li J., Hu N., Li Y., Zhang D., Guo T., Liu X, & Yang W. (2015). Scutellarin’s Cardiovascular Endothelium Protective Mechanism: Important Role of PKG-Iα. PLoS ONE, 10(10), e0139570.

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Vasodilation Evaluation Protocol

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SCU was obtained from Kunming Longjin Pharmaceutical Co. (Kunming, China). Cell culture reagents DMEM, modified RPMI-1640 medium, and fetal bovine serum were obtained from the Hyclone (Thermo Scientific, USA). Other items include Wire Myograph System DMT (Danish Myo Technology Company, Denmark) and Power Lab data recording and analytical system (ADInstruments Ltd., Australia). HBMECs were purchased from Yangsen Biology Limited Company (Shanghai, China). U46619 was purchased from Cayman Chemical Company. PKG inhibitor Rp-8-Br-cGMPS was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), triphenyl tetrazolium chloride (TTC), and ACh were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Du X., Chen C., Zhang M., Cai D., Sun J., Yang J., Hu N., Ma C., Zhang L., Zhang J, & Yang W. (2015). Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 430271.

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