Blood was taken from vena jugularis from nine male Holstein Friesian calves at the age of 6 months. For blood drawing, 9 ml K3E K3EDTA-Vacuette tubes (Greiner bio-one, Germany) and single use needles (20G x 1½”, Greiner bio-one, Germany) were used. Plasma was separated from other blood components directly after blood sampling (15 min for 3500×rcf at room temperature with transportable centrifuges EBA20, Hettich, Germany) and stored at -80°C until RNA extraction. Blood collection was approved by the ethical committee of the Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen (Recklinghausen, Germany) (permit number 84-02.04.2012.A040). Animals were housed and fed according to good animal attendance practice and all efforts were made to minimize suffering.
K3e k3edta vacuette tubes
Manufactured by Greiner
Sourced in Germany
The K3E K3EDTA-Vacuette tubes are laboratory collection tubes used for the collection of blood samples. They contain the anticoagulant K3EDTA, which prevents the blood from clotting during the collection process. The tubes are designed for single-use and are intended for the collection of venous blood samples.
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Lab products found in correlation
2 protocols using k3e k3edta vacuette tubes
1
Blood Sampling from Holstein Friesian Calves
2
Plasma Isolation from Whole Blood
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To generate plasma, peripheral whole blood was collected from the jugular vein using 9 ml K3E K3EDTA-Vacuette tubes (Greiner bio-one, Germany) and single use needles (Greiner bio-one, Germany) with a subsequent separation of cellular components by centrifugation for 15 min at 3500 × rcf at room temperature with transportable centrifuges (EBA20, Hettich, Germany). Plasma was stored at −80 °C until RNA extraction. Samples were taken at d + 17 after the initial treatments.
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