Log In Sign up
The largest database of trusted experimental protocols

Dotslide scanner vs110

Manufactured by Olympus
Visit Supplier

The Dotslide Scanner VS110 is a high-performance digital slide scanner designed for the examination of microscopic samples. It captures detailed images of specimens and supports various slide formats. The device features advanced optics and an efficient scanning process to provide users with accurate and reliable results.

Automatically generated - may contain errors

Lab products found in correlation

Biotinylated secondary antibody, by Vector Laboratories (5 mentions) 3 3 diaminobenzidine (dab), by Agilent Technologies (4 mentions) Shandon varistain 24 4 automatic slide stainer, by Thermo Fisher Scientific (4 mentions) Ab150686, by Abcam (3 mentions) Primary antibody against zeb1, by Merck Group (2 mentions) Gp62 c, by Progen Biotechnik (2 mentions) Trichrome stain, by Abcam (1 mentions) Hif 1α, by Cayman Chemical (1 mentions)

Spelling variants of this product

VS110 (27 citations) DotSlide (19 citations) DotSlide scanner (3 citations)

5 protocols using dotslide scanner vs110

1

Immunohistochemistry of ZEB1 in Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Control or IPF lung tissues (n = 3 donors) were fixed and embedded in paraffin wax; tissue sections (4 µm) were processed and stained as previously described [20 (link)]. Briefly, the tissue sections were de-waxed, rehydrated and incubated with 3% hydrogen peroxide in methanol for 10 min to block endogenous peroxidase activity. Sections were then blocked with normal goat serum and incubated at room temperature with a primary antibody against ZEB1 (1:500, Sigma), followed by a biotinylated secondary antibody (1:500, Vector Laboratories Ltd., UK); antibody binding was detected using streptavidin-conjugated horse-radish peroxidase and visualised using DAB (DAKO) before counterstaining with Mayer’s Haematoxylin. For H/E stain, Shandon Varistain 24-4 automatic slide stainer (Thermo Fisher Scientific) was used. For tinctorial stain, Trichrome stain (Abcam ab150686) was used according to the manufacturers’ instructions. Images were acquired using an Olympus Dotslide Scanner VS110.
Yao L., Conforti F., Hill C., Bell J., Drawater L., Li J., Liu D., Xiong H., Alzetani A., Chee S.J., Marshall B.G., Fletcher S.V., Hancock D., Coldwell M., Yuan X., Ottensmeier C.H., Downward J., Collins J.E., Ewing R.M., Richeldi L., Skipp P., Jones M.G., Davies D.E, & Wang Y. (2018). Paracrine signalling during ZEB1-mediated epithelial–mesenchymal transition augments local myofibroblast differentiation in lung fibrosis. Cell Death and Differentiation, 26(5), 943-957.
+ Open protocol
+ Expand
2

Immunohistochemical Profiling of p62/SQSTM1 in Lung Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Control or IPF lung tissues were fixed and embedded in paraffin wax;
tissue sections (4µm) were processed and stained as previously
described18 . Briefly,
the tissue sections were de-waxed, rehydrated and incubated with 3% hydrogen
peroxide in methanol for 10 min to block endogenous peroxidase activity.
Sections were then blocked with normal goat serum and incubated at room
temperature with a primary antibody against p62/SQSTM1 (1:100, Progen, GP62_C),
followed by a biotinylated secondary antibody (1:500, Vector Laboratories Ltd,
UK); antibody binding was detected using streptavidin-conjugated horse-radish
peroxidase and visualised using DAB (DAKO) before counterstaining with
Mayer's Haematoxylin. For H/E stain, Shandon Varistain 24-4 automatic
slide stainer (Thermo Fisher Scientific) was used. For tinctorial stain,
Trichrome stain (Abcam ab150686) was used according to the manufacturers’
instructions. Images were acquired using an Olympus Dotslide Scanner VS110.
Hill C., Li J., Liu D., Conforti F., Brereton C.J., Yao L., Zhou Y., Alzetani A., Chee S.J., Marshall B.G., Fletcher S.V., Hancock D., Ottensmeier C.H., Steele A.J., Downward J., Richeldi L., Lu X., Davies D.E., Jones M.G, & Wang Y. (2019). Autophagy inhibition-mediated epithelial–mesenchymal transition augments local myofibroblast differentiation in pulmonary fibrosis. Cell Death & Disease, 10(8), 591.
+ Open protocol
+ Expand
3

Immunohistochemical Analysis of p62 in IPF Lungs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Control or IPF lung tissues were fixed and embedded in paraffin wax; tissue sections (4 µm) were processed and stained as previously described18 (link). Briefly, the tissue sections were de-waxed, rehydrated and incubated with 3% hydrogen peroxide in methanol for 10 min to block endogenous peroxidase activity. Sections were then blocked with normal goat serum and incubated at room temperature with a primary antibody against p62/SQSTM1 (1:100, Progen, GP62_C), followed by a biotinylated secondary antibody (1:500, Vector Laboratories Ltd., UK); antibody binding was detected using streptavidin-conjugated horse-radish peroxidase and visualised using DAB (DAKO) before counterstaining with Mayer’s Haematoxylin. For H/E stain, Shandon Varistain 24-4 automatic slide stainer (Thermo Fisher Scientific) was used. For tinctorial stain, Trichrome stain (Abcam ab150686) was used according to the manufacturers’ instructions. Images were acquired using an Olympus Dotslide Scanner VS110.
Hill C., Li J., Liu D., Conforti F., Brereton C.J., Yao L., Zhou Y., Alzetani A., Chee S.J., Marshall B.G., Fletcher S.V., Hancock D., Ottensmeier C.H., Steele A.J., Downward J., Richeldi L., Lu X., Davies D.E., Jones M.G, & Wang Y. (2019). Autophagy inhibition-mediated epithelial–mesenchymal transition augments local myofibroblast differentiation in pulmonary fibrosis. Cell Death & Disease, 10(8), 591.
+ Open protocol
+ Expand
4

Immunohistochemical Analysis of CA-IX and HIF1α

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Control or IPF lung tissues (n = 3 donors) were fixed and embedded in paraffin wax; tissue sections (4 μm) were processed and stained as previously described (Yao et al., 2019 (link); Hill et al., 2019b (link)). Briefly, the tissue sections were de-waxed, rehydrated and incubated with 3% hydrogen peroxide in methanol for 10 min to block endogenous peroxidase activity. Sections were then blocked with normal goat serum and incubated at room temperature with a primary antibody against CA-IX (1:500, Novus Biologicals, Cambridge, UK) or HIF1α (1:500, Cayman Chemical, Michigan, USA), followed by a biotinylated secondary antibody (1:500, Vector Laboratories Ltd., UK); antibody binding was detected using streptavidin-conjugated horse-radish peroxidase and visualised using DAB before counter-staining with Gill’s Haematoxylin. Images were acquired using an Olympus Dotslide Scanner VS110.
Brereton C.J., Yao L., Davies E.R., Zhou Y., Vukmirovic M., Bell J.A., Wang S., Ridley R.A., Dean L.S., Andriotis O.G., Conforti F., Brewitz L., Mohammed S., Wallis T., Tavassoli A., Ewing R.M., Alzetani A., Marshall B.G., Fletcher S.V., Thurner P.J., Fabre A., Kaminski N., Richeldi L., Bhaskar A., Schofield C.J., Loxham M., Davies D.E., Wang Y, & Jones M.G. (2022). Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis. eLife, 11, e69348.
+ Open protocol
+ Expand
5

Immunohistochemical Analysis of ZEB1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Control or IPF lung tissues (n = 3 donors) were fixed and embedded in paraffin wax; tissue sections (4µm) were processed and stained as previously described 20 (link). Briefly, the tissue sections were de-waxed, rehydrated and incubated with 3% hydrogen peroxide in methanol for 10 min to block endogenous peroxidase activity. Sections were then blocked with normal goat serum and incubated at room temperature with a primary antibody against ZEB1 (1:500, Sigma), followed by a biotinylated secondary antibody (1:500, Vector Laboratories Ltd, UK); antibody binding was detected using streptavidin-conjugated horse-radish peroxidase and visualised using DAB (DAKO) before counterstaining with Mayer's Haematoxylin. For H/E stain, Shandon Varistain 24-4 automatic slide stainer (Thermo Fisher Scientific) was used. For tinctorial stain, Trichrome stain (Abcam ab150686) was used according to the manufacturers’ instructions. Images were acquired using an Olympus Dotslide Scanner VS110.
Yao L., Conforti F., Hill C., Bell J., Drawater L., Li J., Liu D., Xiong H., Alzetani A., Chee S.J., Marshall B.G., Fletcher S.V., Hancock D., Coldwell M., Yuan X., Ottensmeier C.H., Downward J., Collins J.E., Ewing R.M., Richeldi L., Skipp P., Jones M.G., Davies D.E, & Wang Y. (2018). Paracrine signalling during ZEB1-mediated epithelial–mesenchymal transition augments local myofibroblast differentiation in lung fibrosis. Cell Death and Differentiation, 26(5), 943-957.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!