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18 protocols using disodium hydrogen phosphate dodecahydrate

1

Chitosan-Based Nanoparticle Fabrication

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Low molecular weight chitosan (CS), sodium tripolyphosphate (TPP) penta basic, dimethyl sulfoxide (DMSO), 3,3′,5,5′-tetramethylbenzidine (TMB), sodium citrate dihydrate, hydrogen tetrachloroaurate (III) tetrahydrate (HAuCl4·4H2O), hydrogen peroxide (H2O2), hydroxylammonium chloride (NH2OH·HCl), sodium carbonate anhydrous (Na2CO3), sodium hydrogen carbonate (NaHCO3), disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O), sodium dihydrogenphosphate dihydrate (NaH2PO4·2H2O), acetic acid (HAc), and sodium acetate (NaAc) were all purchased from Merck. All reagents were of analytical grade and used without any further purification. Ninhydrin, hydrindatin were purchased from Ingos (Prague, Czech Republic). Aqueous solutions for size analysis were prepared using PURELAB® Ultra (Elga, High Wycombe, United Kingdom) resistivity 18 mΩ-cm. For other purposes deionized water was used.
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2

Fenofibrate Formulation Development

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Fenofibrate was kindly donated from JPI Co. (Riyadh, Saudi Arabia). Polyvinylpyrrolidone, Kollidon® 12 and D-α-Tocopherol polyethylene glycol 1000 succinate, and Kolliphor® TPGS were obtained as a gift sample from BASF Co. (Ludwigshafen, Germany). Microcrystalline cellulose and Avicel® PH-102 were gained as a gift sample from DuPont Nutrition USA, Inc. (Wilmington, NC, USA). Lactose monohydrate and Granulac 200® were obtained from Meggle (Wasserburg, Germany). Colloidal silicon dioxide and Aerosil 200® were purchased from Evonik (Hanau-Wolfgang, Germany). Sodium stearyl fumarate, Pruv® and sodium starch glycolate, and Explotab® were obtained as a gift sample form JRS pharma (Rosenberg, Germany). Sodium dihydrogen phosphate dehydrate, disodium hydrogen phosphate dodecahydrate and sodium hydroxide were purchased from Merck (Darmstadt, Germany).
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3

Characterization of Turkish Honey

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Honey samples were obtained from Ombulak village in Tuzluca (Iğdır, Turkey). Honey samples were of multifloral origin and were harvested in July 2019 season. α-glucosidase, p-nitrophenyl-α-Dglucopyranoside substrate, D(+) glucose, D(+) fructose and genistein were from from Sigma Chemical Co. (Steinheim, Germany). 1,1-diphenyl-2-picrylhydrazyl (DPPH), sodium nitrite, potassium nitrate, formic acid, sodium sulfate, sodium hydroxide, sodium carbonate, sodium dihydrogen phosphate dihydrate and disodium hydrogen phosphate dodecahydrate were from Merck (Darmstadt, Germany). Glycylglycine were from Fluka (Buchs, Switzerland) and acetonitrile was obtained from J. T. Baker (Deventer, Netherlands). All reagents were used without any further purification
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4

Hydrophilic Polymer Additive Formulation

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Hydroxypropyl cellulose (HPC; Klucel™ LF, Ashland, US-MA; two different batches indicated as 1 and 2); caffeine (ACEF, I); polylactic acid (PLA) filament (L-PLA natural, ø 1.75 mm; MakerBot ® Industries, LLC, US-NY); blue and yellow dye-containing powder products ready for use (Kollicoat ® IR Brilliant Blue and Kollicoat ® IR yellow, BASF, D); deuterium oxide (Aldrich; 99.9 % D); ethylene glycol (Aldrich, anhydrous, 99.8%, I); di-sodium hydrogen phosphate dodecahydrate (Merck KGaA, 99%, D); methanol (Sigma-Aldrich, ACS reagent, reg. ISO, reag. Ph.
Eur., ≥ 99.8%, I); poly(ethylene glycol)/poly(ethylene oxide) (PSS Polymer standards service GmbH, D); sodium phosphate monobasic monohydrate (Aldrich, puriss. p.a., ACS reagent, ≥ 99.0%, I); water (Aldrich, HPLC grade, I).
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5

Comparative Analysis of Raw Milk Composition

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Raw buffalo and bovine milk were provided by Floridia Cheese (Thomastown, VIC, Australia) and Saputo Dairy Australia (Laverton North, VIC, Australia), respectively. Raw milk was added with 0.02% sodium azide (CAS No. 26628-22-8; Sigma-Aldrich, St. Louis, MO, USA) and stored under refrigerated conditions until analysis the next day. Trisodium citrate dihydrate (CAS No. 6132-04-3; Na3C6H5O7∙2H2O) and disodium hydrogen phosphate dodecahydrate (CAS No. 10039-32-4; Na2HPO4∙12H2O) were purchased from Sigma-Aldrich (Australia).
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6

Recombinant AR Enzyme Assay Protocol

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Human recombinant AR was supplied by Wako Pure Chemical Industries Ltd. (Osaka, Japan). Ammonium sulfate, disodium hydrogen phosphate dodecahydrate, DL-glyceraldehyde, NADPH, potassium dihydrogen phosphate, quercetin, sodium dihydrogen phosphate dehydrate, sodium hydroxide, and trifluoroacetic acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). HE, EA, and ME were purchased from J.T. Baker (Phillipsburg, NJ, USA). The deionized WA (resistivity > 18.2 MΩ cm) was produced by a Milli-Q system (Millipore, Bedford, MA, USA) in our laboratory.
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7

Synthesis of Colloidal Quantum Dots

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Cadmium nitrate tetrahydrate (Cd(NO3)2·4H2O, >98%), sodium myristate (Na(myr), >99%), 1-octadecene (ODE, 90%), cadmium acetate dihydrate (Cd(OAc)2·2H2O, >98%), oleic acid (OLA, >90%), n-hexane (≥99%), 11-mercaptoundecanoic acid (MUA, 95%), acetone (99.5%), potassium hydroxide (KOH, >85%), ammonium hydroxide (NH4OH, 28–30%), hydrogen peroxide (H2O2, 30%), toluene (99.7%), (3-mercaptopropyl)trimethoxysilane (MPTMS, 95%), sulfuric acid (H2SO4) (95–97%), potassium ferricyanide(iii) trihydrate (≥98.5%), sodium dihydrogen phosphate dihydrate (99%), and disodium hydrogen phosphate dodecahydrate (99%) were purchased from Sigma-Aldrich. Methanol (MeOH, ≥99.8%), 2-propanol (99.5%), and ethanol (EtOH, ≥99.8%) were purchased from Roth. Selenium (200 mesh, 99.999%) was purchased from Alfa Aesar. Sodium sulfite (Na2SO3) (>97%) was purchased from Fisher Scientific. All chemicals were used as received without further purification.
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8

Quantification of Antioxidant Compounds

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Glycerin, D-glucose, lactic acid, and Trisodium Citrate Dihydrate were purchased from GHTECH Co. (Guangdong Guanghua, China). Choline chloride was purchased from Himedia Co. (Dindori, Nashik, India). L-(+)-tartaric acid and citric Acid Monohydrate were purchased from Xilong Scientific Co. (Shantou, Guangdong, China). Perchloric acid was purchased from Alpha Chemika Co. (Andheri West, Mumbai, Maharashtra, India). Vanilin was purchased from Shanghai Zhanyun Chemical Co. (Shanghai, Shanghai, China). Folin’s reagent (purity ≥ 1.9N), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, aluminum chloride, potassium acetate, ethanol, sodium dihydrogen phosphate dihydrate, salicylic acid, 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, disodium hydrogen phosphate dodecahydrate, starch, and cellulast 1.5L were purchase from Sigma Chemical Co. (St. Louis, MO).
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9

Chromatographic Determination of Neostigmine

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Analytical grade sodium dihydrogenphosphate and disodium hydrogenphosphate dodecahydrate were obtained from Sigma-Aldrich (Budapest, Hungary). High-performance liquid chromatography (HPLC) grade glacial acetic acid, acetonitrile, and methanol were purchased from Merck (Darmstadt, Germany). Benzyltri-n-propylammonium chloride (internal standard) and neostigmine methylsulfate were from Sigma-Aldrich. The water used was purified and deionized with the Milli-Q system (Millipore, Milford, MA, USA).
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10

Polymer-based Drug Delivery System

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Hydroxyethyl methacrylate (HEMA), Methacrylic acid (MAA), Dimethylaminoethyl methacrylate (DMAEMA), 5-amino salicylic acid, ethylene glycol dimethacrylate (EGDMA), disodium hydrogen phosphate dodecahydrate, azobisisobutyronitrile (AIBN), potassium chloride, sodium chloride, potassium dihydrogen phosphate, BRAND® stopcock grease, sodium dodecyl sulphate, and sodium hydroxide were purchased from Sigma-Aldrich. Capmul® MCM C8 was purchased from ABITEC. Hydrochloric acid (37%) was purchased from Biosolve Chimie. HPLC-grade water was used in all experiments. The purchased materials were used as supplied with no modification.
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