Equal protein amounts from isolated cardiomyocytes, rat heart, and isolated mitochondria were resolved by 8-12% SDS-PAGE and transferred to polyvinylidene fluoride membrane for immunoblot analysis, as previously described [26 (link)]. Membranes were blocked with 5% nonfat milk in Tris-Buffered Saline- (TBS-) Tween and were incubated with primary antibodies overnight at 4°C at the following dilutions: AMPK, phosphorylated AMPK (Thr172), STAT3, phosphorylated STAT3 at Tyr705 (p-STAT3 Tyr705), Bax, Bcl2, caspase-3, and cleaved caspase-3 (Cell Signaling Technology, Beverly, MA) 1 : 1000; phosphorylated STAT3 at Ser727 (p-STAT3 Ser727) (Cell Signaling Technology, Beverly, MA) 1 : 500. After washing with TBS-Tween, immunoreactive bands were visualized by an enzymatic chemiluminescence method and quantified with Quantity One image software.
Phosphorylated stat3 at tyr705
Manufactured by Cell Signaling Technology
Sourced in United States
Phosphorylated STAT3 at Tyr705 is a lab equipment product that detects the phosphorylation of the transcription factor STAT3 at the tyrosine 705 residue. This product is used to measure the activation of the STAT3 signaling pathway in various experimental models.
Automatically generated - may contain errors
Lab products found in correlation
Caspase 3, by Cell Signaling Technology (1 mentions)
Phosphorylated ampk thr 172, by Cell Signaling Technology (1 mentions)
P stat3 tyr705, by Cell Signaling Technology (1 mentions)
P stat3 ser727, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Ipgell, by Genostaff (1 mentions)
Vectastain elite kit, by Vector Laboratories (1 mentions)
Apoptag peroxidase in situ apoptosis detection kit, by Merck Group (1 mentions)
2 protocols using phosphorylated stat3 at tyr705
1
Western Blot Analysis of Cardiac Proteins
2
Comprehensive Tissue Characterization Protocol
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The tissue specimens were fixed in 4% paraformaldehyde, embedded in paraffin, and cut into 4-μm thick sections. Crypts were isolated by EDTA, and organoids were fixed in 4% paraformaldehyde and embedded in iPGell (Genostaff, Tokyo, Japan). The sections were stained with H&E. For immunohistochemistry, antibodies against Ki-67 (Thermo Fisher Scientific), CD44s (Merck), phosphorylated Stat3 at Tyr705 (Cell Signaling Technology, Danvers, MA, USA), and phosphorylated FAK at Tyr397 (Cell Signaling Technology) were used as the primary antibodies. Staining signals were visualized using a Vectastain Elite Kit (Vector Laboratories, Burlingame, CA, USA). Apoptosis was examined using an Apoptag Peroxidase In Situ Apoptosis Detection Kit (Merck).
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