Normal human astrocytes (NHA) were maintained in astrocyte media (Welgene) supplemented with 10% fetal bovine serum (FBS; HyClone, Thermo Fisher Scientific), and 1% penicillin‐streptomycin (Welgene). Glioma cells (A172, A1207, and U87MG) were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 (Welgene) supplemented with 10% fetal bovine serum (FBS; HyClone, Thermo Fisher Scientific) and 1% penicillin‐streptomycin (Welgene). GBM patient‐derived glioma stem cells (GSC11, GSC20, GSC23, and GSC267) were provided by The University of Texas MD Anderson Cancer Center. GSCs were cultured under stem cell culture conditions (NBE, serum‐free neurobasal media supplemented with growth factors). The NBE comprised DMEM/F12 (Welgene) supplemented with B27 (Gibco), 1% penicillin/streptomycin (Welgene), epidermal growth factor (EGF; 20 ng/mL; R&D Systems), and basic fibroblast growth factor (bFGF; 20 ng/mL; R&D Systems).14 Growth factors (bFGF and EGF) were added twice a week. To induce differentiation, GSC11 and GSC23 cells were cultured for 10 days in DMEM/F12 containing 10% FBS.
Penicillin streptomycin
Manufactured by Welgene
Sourced in United States, Cameroon, United Kingdom
Penicillin/streptomycin is a combination of two antibiotics commonly used in cell culture media. It serves to prevent bacterial contamination in cell culture experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Welgene (128 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (103 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (51 mentions)
Rpmi 1640, by Welgene (36 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (33 mentions)
Dulbecco s modified eagle s medium, by Welgene (31 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (27 mentions)
Rpmi 1640 medium, by Welgene (25 mentions)
Phosphate buffered saline (pbs), by Welgene (19 mentions)
Dulbecco s modified eagle s medium dmem, by Welgene (17 mentions)
Trypsin edta, by Welgene (17 mentions)
L glutamine, by Welgene (15 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (14 mentions)
Lipopolysaccharides (lps), by Merck Group (14 mentions)
Dmem f12, by Welgene (13 mentions)
Insulin, by Merck Group (13 mentions)
Minimum essential medium (mem), by Welgene (11 mentions)
Dmem f12, by Thermo Fisher Scientific (11 mentions)
Sodium pyruvate, by Welgene (10 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (10 mentions)
417 protocols using penicillin streptomycin
1
Culturing Astrocytes and Glioma Cells
2
Culturing Glioblastoma Stem Cells and Normal Astrocytes
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Normal human astrocytes (NHA) were purchased from ScienceCell Research Laboratories (USA) and cultured in Astrocyte Medium (ScienCell Research Laboratories, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (Welgene, Korea). GBM patient-derived GSC11 and GSC23 were obtained from the University of Texas MD Anderson Cancer Center.22 (link) GSCs were maintained using neurobasal medium (NBE, serum-free Neurobasal media supplemented with basic FGF and EGF). NBE conditions were as follows: Dulbecco’s modified Eagle’s medium (DMEM)/F12 (Welgene) was supplemented with 2% B27 (50×) (Gibco), 1% penicillin/streptomycin (Welgene), epidermal growth factor (EGF; 20 ng/mL; R&D Systems, USA), and basic fibroblast growth factor (bFGF; 20 ng/mL; R&D Systems). Serum-free conditions were as follows: Dulbecco’s modified Eagle’s medium (DMEM)/F12 (Welgene) was supplemented with 5% UltraGROTM–Advanced Cell Culture Supplement (AventaCell) and 1% penicillin/streptomycin (Welgene).
3
Cultivation of Breast Cancer Cell Lines
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The human breast cancer cell lines BT-549, HS578T, MDA-MB-231, MDA-MB-468, T47D, and MCF-7 were obtained from the National Cancer Institute (NCI, USA). One human breast cancer cell line (HCC-70) was obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea). MDA-MB-231 and MCF-7 human breast cancer cell lines were maintained in DMEM/HIGH GLUCOSE (HyClone, Marlborough, MA, USA) with 10% fetal bovine serum (FBS, HyClone) supplemented with 1% penicillin–streptomycin (Welgene, Daegu, Republic of Korea) and MycoZap (Lonza, Basel, Switzerland). BT-549, HS578T, MDA-MB-468, T47D, and HCC-70 human breast cancer cell lines were maintained in RPMI-1640 medium (HyClone) supplemented with 10% FBS (HyClone) supplemented with 1% penicillin–streptomycin (Welgene) and MycoZap (Lonza). All breast cancer cell lines were cultured in a 5% CO2 incubator at 37 °C. A stable MCF-7 cell line overexpressing AXL was produced via puromycin selection. Human umbilical vein endothelial cells (HUVECs; Lonza and Yale VBT Core, New Haven, CT, USA) were cultured in a 5% CO2 incubator at 37 °C with EBM-2 basal medium supplemented with EGM-2 (Lonza) with 1% penicillin–streptomycin (Welgene). HUVECs (passages 3–7) were grown to 70% to 90% confluence.
4
Immortalized Hepatocyte Culture Protocol
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Immortalized embryonic hepatocytes were cultured in Medium 199 (WelGENE) that was supplemented with 10% fetal bovine serum (WelGENE) and 1% penicillin-streptomycin (WelGENE), as previously described (Back et al., 2009 (link)). AML12 muse normal hepatocytes were obtained from the American Type Culture Collection (ATCC, USA). The cells were cultured in DMEM/F12 (WelGENE) that was supplemented with 10% fetal bovine serum (WelGENE), 100 nM dexamethason (Sigma-Aldrich), 1% insulin-transferrin-selenium-pyruvate supplement (ITSP; WelGENE), and 1% penicillin-streptomycin (WelGENE). The Lenti-X 293T Cell Line (Clontech, USA) was cultured in DMEM (WelGENE) containing 10% fetal bovine serum (WelGENE) and 1% penicillin-streptomycin (WelGENE).
5
Cell Culture Conditions for In Vitro Studies
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YAC-1, CT26, 4T1, B16F10, Jurkat, EL4, AU565 and K562 cells were cultured in RPMI 1640 (WelGENE) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Gibco). NK92 cells were cultured in alpha-MEM (WelGENE) supplemented with 10% FBS, 1% penicillin/streptomycin and hIL-2 (10 ng/ml). HEK293T cells were cultured in DMEM (WelGENE) supplemented with 10% FBS and 1% penicillin/streptomycin. U87MG cells were cultured in MEM (WelGENE) supplemented with 10% FBS and 1% penicillin/streptomycin. Cells were incubated at 37 ℃ and humidified 5% CO2 conditions.
6
Endothelial Cell Culture and Transfection
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PAECs (Lonza, Basel, Switzerland) were cultured at 37 °C in a 5% CO2 incubator in endothelial cell growth medium-2 (Lonza), 1% penicillin–streptomycin (Welgene, Daegu, Republic of Korea), and MycoZap (Lonza). For the experimental treatments, PAECs were grown to 70–90% confluency. Lenti-HEK293X cells were cultured in Dulbecco’s modified Eagle’s medium (HyClone, USA) supplemented with 10% fetal bovine serum (Gibco, USA) and 1% penicillin–streptomycin (Welgene). Small interfering RNA (siRNA) (Stealth siRNA; Invitrogen, Grand Island, NY, USA) was transfected using Lipofectamine RNAi MAX (Invitrogen) or DharmaFECT4 according to the manufacturer’s instructions. Plasmid DNAs were transfected into cells using Lipofectamine 2000 (Invitrogen).
7
HUVEC, MDA-MB-231, and HEK-293 Cell Culture
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HUVECs (Human umbilical vein ECs; Lonza and Yale VBT Core) were maintained in EBM-2 basal medium supplemented with EGM-2 (Lonza) with 1% penicillin/streptomycin (Welgene). HUVECs were grown to 70% to 90% confluency and used between passages 4 to 7 for all experiments. MDA-MB-231 (human breast adenocarcinoma cells) were maintained in DMEM (Dulbecco’s modified Eagles medium; Welgene) supplemented with 10% FBS (fetal bovine serum, Hyclone) and 1% penicillin-streptomycin (Welgene). HEK-293 cells (human embryo kidney cells) were maintained in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin. Cells were maintained in a 5% CO2 incubator at 37°C. siRNA (Bioneer), miRNA mimics and anti-miRs (miRVana; Ambion) were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions.
8
Colorectal Cancer Cell Line and Lymphocyte Culture
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The human colorectal adenocarcinoma cell line HT29 was purchased from the American Type Culture Collection (ATCC) and maintained in RPMI1640 with stable glutamine (Biowest) enriched with 10% fetal bovine serum (FBS)(Biowest), 1% penicillin/streptomycin (Welgene), and 25 mM HEPES (Shimakyu Co., Ltd., Japan), 25 mM NaHCO3 (Shimakyu Co., Ltd.,) at 37°C with 5% CO2. Isolated MNL lymphocytes were cultured in RPMI 1640 with L-glutamine and sodium bicarbonate (Sigma), 10% FBS (Biowest), 1% penicillin/streptomycin (Welgene), and 0.05 mM 2-β-mercaptoethanol (Sigma) at 37°C in the presence of 5% CO2. For western blot analysis, Gos/Fos, inulin, and β-glucan were treated in the presence of 0, 0.25, 0.5, 1, 2, 5 % w/v, respectively for 12 h.
9
Culturing and Transfecting Human Endothelial Cells
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Human umbilical vein endothelial cells (HUVECs; Lonza, Basel, Switzerland and Yale VBT Core, New Haven, USA) were cultured at 37°C in a 5% CO2 incubator with EBM-2 basal medium supplemented with EGM-2 (Lonza) and 1% penicillin–streptomycin (Welgene, Daegu, Korea). For experimental treatments, HUVECs (passages 3–7) were grown to 70% to 90% confluence. HEK293T cells were cultured in Dulbecco's modified Eagle's medium (Hyclone, Logan, USA) supplemented with 10% fetal bovine serum (Gibco, Grand Island, USA) and 1% penicillin–streptomycin (Welgene). small interfering RNA (siRNA) (Stealth siRNA; Invitrogen, Grand Island, USA) and microRNA (miRNA) mimics (miRVanaTM; Ambion, MA, USA) were transfected with Lipofectamine RNAiMAX (Invitrogen) as per the manufacturer's instructions.
10
Culturing Human and Murine Cells
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Primary human epidermal keratinocytes (HEKn; American Type Culture Collection [ATCC], Manassas, VA, USA) were cultured in growth medium (GM) composed of dermal cell basal medium (ATCC) supplemented with a keratinocyte growth kit (ATCC). Murine macrophages (RAW 264.7 cells, Korea Cell Line Bank, Seoul, Republic of Korea) were cultured in Dulbecco’s modified Eagle medium (HyClone-Cytiva, Marlborough, MA, USA) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, Rockford, IL, USA) and 1% penicillin/streptomycin (Welgene, Gyeongsan, Republic of Korea). Human dermal fibroblasts (CCD-986Sk; ATCC) were cultured in Iscove′s modified Dulbecco′s medium (Welgene) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. All cell types were maintained at 37 °C with 5% CO2.
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