Blood samples were taken from participants who had fasted for 10 hours between 7:00 a.m. and 10:00 a.m. The levels of FT3, FT4, TSH, thyroid peroxidase antibodies (TPOAb), and thyroglobulin antibody (TgAb) in the serum were analyzed using the Microparticle Enzyme Immunoassay (MEIA) technique on the UniCel Dxi 800 Access instrument (Beckman Coulter, USA). We used the normal reference ranges for thyroid function and thyroid antibodies recommended by manufacturers: TSH (0.56-5.91 pmol/L), FT3 (3.53-7.37 pmol/L), FT4 (7.98-16.02 pmol/L), TPOAb (0-9 IU/mL), and TgAb (0-4.9 IU/mL) (17 (link), 18 (link)). Fasting plasma glucose (FPG), 2-h postprandial plasma glucose (PPG), creatinine, serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured by the automatic biochemical analyzer AU5831 (Beckman Coulter, USA). Glycated hemoglobin (HbA1c) levels were determined by high-performance liquid chromatography (HLC-723G8, TOSOH, Japan).
Unicel dxi 800 access
Manufactured by Beckman Coulter
Sourced in United States
The UniCel DxI 800 Access is a fully automated immunoassay system designed for clinical laboratories. It offers high-throughput capabilities and is capable of performing a wide range of immunoassay tests.
Automatically generated - may contain errors
4 protocols using unicel dxi 800 access
1
Thyroid Function and Metabolic Biomarkers in Fasted Adults
2
Liver Cancer Biomarker Analysis
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Serum AFP, carcinoembryonic antigen (CEA), cancer antigen 125 (CA-125), and cancer antigen 199 (CA-199) were analyzed on the Cobas e801 platform (Roche, Rotkreuz, Switzerland) and UniCel DxI 800 Access (Beckman Coulter, Brea, California, USA); Protein induced by vitamin K absence or antagonist-II (PIVKA-II) was analyzed on the ARCHITECT i2000SR platform (Abbott Park, Illinois, USA); HBsAg and HBeAg were analyzed on the EasyCuta platform (PerkinElmer, Taicang, Suzhou, China) following the manufacturers’ instruction and laboratory standard operating procedures. Serum AFP level lower than 20 ng/mL was defined as AFP negative, while it was defined as AFP positive if the concentration was higher than 20 ng/mL.
3
Serum Hormone Levels Analysis
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All serum hormone levels were measured by a UniCel DxI 800 Access (Beckman Coulter, USA) in the clinical laboratory of Shantou Central Hospital. Serum E2 concentration was measured with Access Estradiol Kits (Beckman Coulter, USA) based on an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA); serum testosterone concentration was measured with Access Testosterone Kits (Beckman Coulter, USA) based on IC-ELISA; serum AMH concentration was measured with Access AMH Kits (Beckman Coulter, USA) based on an ELISA double sandwich method. All experiments were carried out according to the manufacturer's instructions.
Regression coefficients (R 2 ) of the standard curves of the three hormones all exceeded 0.99. The precision of the method was determined by calculating the relative standard deviation percentage (%RSD) of the quality control samples for repeated measurement, and the relative standard deviation percentages of the three hormones were all determined to be b10%.
Regression coefficients (R 2 ) of the standard curves of the three hormones all exceeded 0.99. The precision of the method was determined by calculating the relative standard deviation percentage (%RSD) of the quality control samples for repeated measurement, and the relative standard deviation percentages of the three hormones were all determined to be b10%.
4
Baseline Characteristics and Biomarkers in Patients
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The baseline clinical characteristics included the patient's gender, age, smoking history, systolic blood pressure (SBP), diastolic blood pressure (DBP), blood glucose (BG), body mass index (BMI = weight (kg)/ [height (m)]2), and past medical histories. The age-adjusted Charlson Comorbidity Index (ACCI) (13 (link), 14 (link)) was introduced to weigh the burden of comorbidities. Blood samples were drawn from fasting patients by venipuncture within 24 h after admission and stored in 3.2% citrated tubes or plain tubes according to the clinical laboratory requirements. Plasma D-dimer and fibrinogen (Fib) were detected by latex agglutination turbidimetry using a STARMAX automatic coagulation apparatus (STAGO, France). The normal range of plasma D-dimer levels was < 0.5 μg/ml. Low-density lipoprotein cholesterol (LDL-C) was measured using clinical laboratory methods (Beckman CX9, USA). C-reactive protein (CRP) was measured based on an immunofluorescence dry quantitative method using an automatic dry immunoassay analyzer (A5000, Boditech Biotechnology, China). B-Type natriuretic peptide (BNP) was measured by UniCel Dxi800 Access (Beckman Coulter, China). Troponin T (TNT) was measured using a Roche Cobas h 232 immunoassay analyzer (Roche Diagnostics, Mannheim, Germany). The left ventricular ejection fraction (LVEF) was estimated using the Simpson method.
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