Mice were anaesthetized (isoflurane 2%, O2 2 L/min) and visualized using the IVIS spectrum imaging system (Perkin Elmer). L-012 (8-amino-4-chloro-7-phenylpyridol[3,4-d]pyridazine-1, 4(2 H, 3H)dione, a chemitransluminescent probe and derivative of luminol, was purchased from Wako Chemical (Neuss, Germany) and dissolved in H2O. A concentration of 75 mg/kg in a volume of 100 μL was administered intravenously. Images were taken with the IVIS spectrum imaging system (Perkin Elmer). For molecular imaging, mice were sacrificed after an hour by an overdose of anaesthesia, and the chest was opened according to standard necropsy protocols. Data acquisition and analysis were performed using IVIS imaging software Living Image (Caliper). The photon flux was quantified within a region of interest encompassing the thoracic chest region of each mouse. The signal was normalized against an illumination profile for the selected field of view. Also see reference. 29 (link)
Ivis spectrum imaging system
Manufactured by PerkinElmer
Sourced in United States, Germany
The IVIS Spectrum imaging system is a high-performance in vivo imaging instrument designed for preclinical research applications. It offers advanced optical imaging capabilities, including bioluminescence, fluorescence, and X-ray imaging, to support a wide range of biological studies.
Automatically generated - may contain errors
Lab products found in correlation
Living image software, by PerkinElmer (39 mentions)
D luciferin, by PerkinElmer (20 mentions)
Living image 4, by PerkinElmer (14 mentions)
D luciferin, by GoldBio (12 mentions)
Living image software 4, by PerkinElmer (9 mentions)
D luciferin, by Promega (8 mentions)
D luciferin, by Thermo Fisher Scientific (7 mentions)
Living image software version 4, by PerkinElmer (7 mentions)
Luciferin, by PerkinElmer (6 mentions)
Ivis living imaging 3, by PerkinElmer (5 mentions)
Lsm 510, by Zeiss (3 mentions)
Aura software, by Spectral Instruments Imaging (3 mentions)
Fv1000, by Olympus (3 mentions)
Living image, by PerkinElmer (2 mentions)
D luciferin solution, by PerkinElmer (2 mentions)
Isoflurane, by Abbott (2 mentions)
Zetasizer nano zs90, by Malvern Panalytical (2 mentions)
D luciferin potassium salt, by Promega (2 mentions)
Cliniplate, by Thermo Fisher Scientific (2 mentions)
D luciferin potassium salt, by PerkinElmer (2 mentions)
349 protocols using ivis spectrum imaging system
1
In Vivo Bioluminescence Imaging in Mice
2
In Vivo Tumor Imaging with DiD-labeled MBP
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When the average tumor volume reached around 200 mm3, the 4T1 tumor-bearing nude mice were intravenously injected with 100 μL of DiD-labeled MBP at a DiD dose of 3 μg and then fluorescence imaging was conducted by an IVIS Spectrum imaging system (PerkinElmer, USA) equipped with a DiD filter set (excitation/emission, 640/670 nm) at various time points (0.5, 1, 2, 4, 6, 8, 20, and 24 h). Twenty-four hours post injection, the mice were sacrificed, followed by heart perfusion with saline, and the major organs (such as heart, liver, spleen, lung and kidney) were sampled for ex vivo fluorescence imaging by the IVIS Spectrum imaging system (PerkinElmer, USA). The fluorescence intensity of the major organs was measured by IVIS Lumina XRMS Series III.
3
Biodistribution of Labeled GH1-Derived Exosomes
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GH1-derived exosomes suspended in PBS were treated with Vybrant DiD Cell-Labeling Solution (Thermo Fisher Scientific). Labeled GH1-exo (5 × 108 particles/mouse) were intravenously injected into female C57BL/6 mice (n = 4), and unlabeled exosomes used as control (n = 1). Two mice injected with DiD-labeled GH1-exo and 1 control mouse were euthanized for organ dissection after 20 hours, as were 2 mice injected with DiD-labeled GH1-exo for 4 hours. Images were taken by using IVIS Spectrum imaging systems (Perkin Elmer).
4
Xenograft Tumor Growth Monitoring
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Female NOD-scidIL2rγnull (NSG) mice (6–7 weeks old; The Jackson Laboratory) were housed at the Emory University HSRB Animal Facility in sterile cages in 12-h/12-h light–dark cycles. All experimental procedures were Emory IACUC approved. We infected SKNDZ cells with GFP/luciferase virus and flow sorted cells, generating the SKNDZ-GFP/luciferase model. We subsequently infected SKNDZ-GFP/luciferase cells with control or shLIN28B lentiviruses, creating stable SKNDZ-GFP-Con, SKNDZ-GFP-shLIN28B-1, and SKNDZ-GFP-shLIN28B-3 models. After acclimatizing, mice were randomized to Con, shLIN28B-1, and shLIN28B-3 groups (n = 10 for all). Respective groups received 1 million Con, shLIN28B-1, or shLIN28B-3 cells via tail vein injection. Starting 14 days post injection, bioluminescence imaging was performed twice a week. For imaging, 150 mg/kg luciferin was intraperitoneally injected into mice 10 minutes prior to imaging with IVIS Spectrum Imaging Systems (Perkinelmer). Imaging settings remained the same throughout the study and luminescence intensity was measured using Living Image Software (Perkinelmer).
5
Angiogenesis Quantification in Tissue Implants
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To assess angiogenesis in the untreated control, plasma gel, non-vascularized and
pre-vascularized groups 14 days after implantation, animals were intravenously injected
with AngioSense 750 EX (Perkin Elmer, Waltham, Massachusetts, USA; 2 nmol/100 μl prepared
in sterile PBS) via tail vein injection15 (link). Fluorescence imaging (excitation: 745 nm; emission: 800 nm; high-pass filter cut
off: 770 nm; illumination: 30%) in the anesthetized animal was performed 24 h after the
injection of the fluorescence dye, using the IVIS Spectrum Imaging System (Perkin Elmer)15 (link).
pre-vascularized groups 14 days after implantation, animals were intravenously injected
with AngioSense 750 EX (Perkin Elmer, Waltham, Massachusetts, USA; 2 nmol/100 μl prepared
in sterile PBS) via tail vein injection15 (link). Fluorescence imaging (excitation: 745 nm; emission: 800 nm; high-pass filter cut
off: 770 nm; illumination: 30%) in the anesthetized animal was performed 24 h after the
injection of the fluorescence dye, using the IVIS Spectrum Imaging System (Perkin Elmer)15 (link).
6
SPIO@SiO2–ICG–RGD Fluorescence Imaging in Mice
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Fluorescence imaging in vivo was undertaken on 10 mice with HF and 10 healthy mice (controls). Approximately 100 μL (0.2 mg mL−1, Fe3O4) of SPIO@SiO2–ICG–RGD was delivered via the tail vein. An IVIS Spectrum imaging system (PerkinElmer) was used to monitor the change in fluorescence signal in mice 0.5, 1, 3, 6, 12, 24, 48, 72, and 96 h post-injection with excitation and emission wavelengths of 790 nm and 828 nm, respectively. Imaging data were analyzed with IVIS Living Image v3.0 (PerkinElmer). The region of interest (ROI) was selected to measure the mean fluorescence intensity (MFI) of the liver. The target:background ratio (TBR) was obtained by dividing the MFI of the liver area by the corresponding body background area. To evaluate the targeting ability of RGD, imaging of the HF of mice treated with SPIO@SiO2–ICG in vivo was done.
7
Bioluminescence Imaging in Mice
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Mice were anesthetized in a chamber with 2.5% isoflurane, along with intraperitoneal injections of D-luciferin (150 mg/kg, Promega). Bioluminescence was measured using an IVIS spectrum imaging system (PerkinElmer) while maintaining 2.5% isoflurane in the imaging chamber via a nose cone. Images were captured 10 min after luciferin administration at the indicated time points. The photon flux values (photons per second), corresponding to the region of interest marked around the bioluminescence signal, were analyzed using Living IMAGE 4.5.2 software (Perkin Elmer, USA).
8
Multimodal Expression Analysis Protocol
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In general, GFP expression was evaluated by fluorescence imaging using an inverted fluorescence microscope (Olympus IX70) and flow cytometry analysis (Attune NXT, Life Technologies). RBD expression was measured using a commercial ELISA kit (SARS-CoV-2 coronavirus spike ELISA kit, Sino Biological). Transmembrane IL-15 expression was measured using fluorescein isothiocyanate (FITC) anti-IL-15 antibody (MA5-23664, Invitrogen) and flow cytometry. Luciferase expression was evaluated by in vivo bioluminescence imaging (IVIS spectrum imaging system, PerkinElmer). The expression of human IFN-a2b, IL-15, GM-CSF, and IL-12sc were determined using commercial ELISA kits (Human IFN-α ELISA kit, Beyotime; Human IL-15 ELISA kit, Abcam; Human GM-CSF ELISA kit, Proteintech; ELISA MAX Deluxe Set Human IL-12 [p70], BioLegend).
9
In Vivo Biodistribution of Hs1a-FL
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One group of animals was intravenously injected with Hs1a-FL (4 nmol, 45 μM of Hs1a-FL in 100 μL of PBS, n = 3). A second group of animals was injected with Hs1a and Hs1a-FL (Hs1a-FL, 45 μM, 4 nmol and Hs1a, 120 μM, 12 nmol in 100 μL PBS, n = 3) or PBS (n = 3). Animals were sacrificed 30 min post-injection and epifluorescence images obtained. Epifluorescence images of the right sciatic nerve (RSN) and left sciatic nerve (LSN) were obtained in situ from all the mice in the study. Epifluorescence images of the biodistribution included RSN, LSN, muscle, heart, kidney, liver, and brain and were acquired with an IVIS Spectrum imaging system (PerkinElmer) using a predetermined filter set (excitation = 710/45 nm, emission = 800–820 nm). Autofluorescence was removed through spectral unmixing. Semiquantitative analysis of the Hs1a-FL signal was conducted by measuring the average radiant efficiency (in units of [p/s/cm2/sr]/[μW/cm2]) in regions of interest (ROIs) that were placed on all resected nerves and as well in all organs from the biodistribution under white light guidance.
10
Xenograft Tumor Growth Monitoring
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Five to six-week-old female BALB/c nude mice were purchased from Vital River (Beijing, China). The mice were bred and maintained under specific pathogen-free conditions, provided with sterilized food and water and housed in a barrier facility with a 12 h light/dark cycle. The mice were randomly divided into two groups and were injected with SGC-7901-shN (n=8) or -sh393 (n=8) cells that expressed TurboGFP. In all, 4×106 cells were injected into the abdominal cavity of the mice along with 250μl PBS. The body weight of the mice was measured every 4 days. Tumor growth and metastasis were monitored in vivo via weekly measurement of the fluorescence intensity by the IVIS Spectrum Imaging System (PerkinElmer, USA). After all mice were sacrificed on day 28, the visible disseminated nodules were counted. The tumors were excised, cut into blocks, fixed in 10% formalin, and embedded in paraffin for IHC or were snap-frozen in liquid nitrogen for western blot.
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