All chemicals not specifically mentioned are from Sigma-Aldrich (highest grade). All inhibitors not specifically mentioned are from Cayman Chemical. The following antibodies were used: Alexa Fluor 647 anti-human CD3 antibody (UCHT1, BioLegend), Alexa Fluor 488 anti-human Granzyme A antibody (CB9, BioLegend), Alexa Fluor 647 or Brilliant Violet (BV) 510 anti-human perforin antibody (dG9, BioLegend), Alexa Fluor 647 anti-human granzyme B antibody (GB11, BioLegend), Brilliant Violet (BV) 421 anti-human CD178 (Fas-L), anti α-Tubulin mAb antibody (DM1A, Cell Signaling Technology), and Alexa Fluor 405 conjugated goat anti-mouse IgG (H+L) cross-absorbed secondary antibody (ThermoFisher Scientific). All isotype controls of fluorescence conjugated primary antibodies are from BioLegend. The following reagents were used: Hoechst 33342 (ThermoFisher Scientific), Alexa Fluor 488 or Alexa Fluor 568 phalloidin (ThermoFisher Scientific), Atto 488 NHS ester ((ThermoFisher Scientific), collagenase type I (ThermoFisher Scientific), FibriCol® type I collagen Solution (Bovine, Advanced Biomatrix).
Atto 488 nhs ester
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Atto 488 NHS ester is a fluorescent labeling reagent used for the covalent attachment of fluorescent dyes to biomolecules, such as proteins, antibodies, or nucleic acids. It contains the Atto 488 fluorophore, which has an absorption maximum at 501 nm and an emission maximum at 523 nm.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Collagenase type 1, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 or alexa fluor 568 phalloidin, by Thermo Fisher Scientific (1 mentions)
Dylight 633 nhs ester, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647, by Thermo Fisher Scientific (1 mentions)
3 protocols using atto 488 nhs ester
1
Multiparametric Flow Cytometry for Immune Cell Analysis
2
Fluorescent Labeling of Biomolecules
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DyLight 633 NHS ester and Atto 488-NHS ester were purchased from ThermoFisher Scientific (USA). All other reported compounds were purchased from Sigma-Aldrich (USA) unless stated otherwise.
3
Oligonucleotide Hapten Conjugation
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Q oligos were conjugated to the following two haptens, 4-hydroxy-3-nitrophenylacetyl-O-succinimide ester (NP-Osu) and 4-hydroxy-3-iodo-5-nitrophenylacetyl amino caproyl acetyl-O-succinimide ester (NIP-eCAP-Osu) (Santa Cruz Biotechnology), Alexa Fluor 647 (AF647) or Atto488 NHS ester (Thermo Scientific). The reactions were carried out in 100 mM HEPES (pH 8.2), ~40% DMSO in a 40-fold excess of the NHS ester at 25 °C, 500 RPM shaking for 4 h.
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