Monkey kidney Cos-7 cells and the plasmids pcDNA3 and pcDNA3-hMTP-FLAG were as described previously (8 (link), 14 (link), 15 (link), 20 (link), 21 (link), 33 (link), 38 (link)). Oligonucleotides were obtained from Integrated DNA Technologies. LB agar (catalog no.: 110283) and LB broth (catalog no.: 110285) for microbiology work were purchased from Millipore. Anti-FLAG M2 affinity agarose gel (catalog no.: A2220-5Ml), monoclonal anti-FLAG M2 antibody (catalog no.: F3165), carbenicillin disodium salt (catalog no.: C3416-5G), and other chemicals were purchased from MilliporeSigma. FLAG peptide was custom synthesized (GenScript). Round bottom black 96-well assay plates were obtained from Costar (catalog no.: 3792; Kennebunk). Human apoB monoclonal antibody, 1D1, was amplified by MyBiosource (catalog no.: MBS465020) for ELISA. Swine anti goat-IgG antibody from Southern Biotech (catalog no.: 6300-04), goat polyclonal anti-hApoB antibody from Academy Bio-Medical Company, Inc (catalog no.: 20S-G2-1.5Ml), and 96-well ELISA assay plates from Corning (catalog no.: 3366) were purchased.
Round bottom black 96 well assay plates
Manufactured by Corning
The Round bottom black 96-well assay plates are a laboratory equipment product designed for use in a variety of scientific applications. They feature a 96-well format with a round bottom well shape. The plates are made of a black material, which can be beneficial for certain types of assays or experiments. This product is intended to provide a standard platform for conducting various laboratory-based tests and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag m2 affinity agarose gel, by Merck Group (2 mentions)
Lb agar, by Merck Group (1 mentions)
Oligonucleotide, by Integrated DNA Technologies (1 mentions)
Anti flag m2 monoclonal antibody, by Merck Group (1 mentions)
Nbd dope, by Avanti Polar Lipids (1 mentions)
Lomitapide, by Merck Group (1 mentions)
2 protocols using round bottom black 96 well assay plates
1
Plasmid Transfection in Cos-7 Cells
2
Fluorescent Lipid Probes and MTP Inhibitor Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PC (no. 131601C) and fluorescently labeled ammonium salts of PEs with the acyl groups, NBD-1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE; no. 810143C), NBD-POPE (no. 810118P), or NBD-DOPE (no. 810145C) were obtained from Avanti Polar Lipids (Alabaster, AL). NBD-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (DPPE; no. N360) was obtained from Invitrogen (Thermo Fisher Scientific). Lomitapide, an MTP inhibitor, was obtained from Sigma (no. SML1385-5MG). The Cos-7 and Huh-7 cell lines, and the plasmids pcDNA3 and pcDNA3-hMTP-FLAG, have been described previously (20 (link), 21 (link), 26 (link), 27 (link)). Anti-FLAG® M2 affinity agarose gel and other chemicals were purchased from Millipore Sigma (no. A2220-5ML, St. Louis, MO). Round bottom black 96-well assay plates were obtained from Costar (no. 3792; Kennebunk, ME). FLAG peptide was custom synthesized (GenScript).
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