Agilent 7890 5975c

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 7890/5975C is a gas chromatograph-mass spectrometer system that is designed for the analysis of complex mixtures of organic compounds. It combines the high separation capabilities of gas chromatography with the sensitive and selective detection of mass spectrometry. The system is capable of identifying and quantifying a wide range of organic compounds in various sample matrices.

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Lab products found in correlation

Pyridine, by Merck Group (2 mentions) Milli q ultrapure water system, by Merck Group (1 mentions) Hplc grade methanol, by Tedia (1 mentions) Centrivap concentrator, by Labconco (1 mentions) Msd chemstation, by Agilent Technologies (1 mentions) U 13c glucose, by Merck Group (1 mentions) Hp 5 capillary column, by Agilent Technologies (1 mentions) Db 5 capillary column, by Agilent Technologies (1 mentions) Methoxyamine, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Elan drc 2, by PerkinElmer (1 mentions)

Close spelling variants of this product

Agilent 7890 A GC/5975C MS system Agilent 7890/5975C GC–MS Agilent 7890/5975C GC/MS system Agilent 7890-5975C GC-MS solution system Agilent 7890 series II and 5975C Agilent 7890 Agilent 5975C

9 protocols using agilent 7890 5975c

GC-MS Analysis of Metabolites

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N-methyl-N-(trimethylsilyl) tri-fluoroacetamide (MSTFA), methoxyamine, pyridine and standard compounds were purchased from Sigma-Aldrich (St. Louis, MO, United States). HPLC grade methanol were purchased from Tedia Co., Inc. (Fairfield, OH, United States). Ultrapure water was produced by the Milli-Q ultra-pure water system (Millipore, Billerica, MA, United States).
Agilent 7890/5975C (single quadrupole mass spectrometer) system (Agilent Technologies, United States) was adopted for GC-MS analysis. An Eppendorf 5804 Rcentrifuge (Hamburg, Germany), a CentriVap Concentrator (LABCONCO, Kansas City, MO, United States) and medical syringes (5 ml, Shandong WEGO Group Medical Polymer Co., Ltd., China) were used.

Shan L., Yang J., Meng S., Ruan H., Zhou L., Ye F., Tong P, & Wu C. (2021). Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry. Frontiers in Molecular Biosciences, 8, 648823.

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Metabolite Quantification by GC-MS

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Metabolites were analyzed on an Agilent 7890/5975C gas chromatography–mass spectrometry system (Agilent Technologies, Santa Clara, CA, USA) using selected ion monitoring methods described in a previous report.⁹ (link) Peaks were integrated in MSD ChemStation (Agilent Technologies), and correction for natural isotope abundance was performed using IsoCor software.¹⁰ (link) Corrected metabolite signals were converted to molar amounts by comparing metabolite peak abundances in samples with those in a “standard mix” containing known quantities of metabolites run along each individual experiment. These known metabolite concentrations were used to generate a standard curve that allowed for metabolite quantification.

Bisbach C.M., Hass D.T., Thomas E.D., Cherry T.J, & Hurley J.B. (2022). Monocarboxylate Transporter 1 (MCT1) Mediates Succinate Export in the Retina. Investigative Ophthalmology & Visual Science, 63(4), 1.

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Lipid Extraction and GC-MS Analysis

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We suspended 1×10⁷ cells in 1 mL of methanol/chloroform and then sonicated them for 30 min. For methylation, 2 mL of 1% sulfuric acid-methanol solution was added to the supernatant at 80 ℃ for half an hour. A total of 1 mL of hexane was used for extraction and washed with 5 mL of pure water. Then, 500 µL of the supernatant was mixed with 25 µL of methyl nonadecanoate as an internal standard and then entered into gas chromatography-mass spectrometry (GC-MS) detection with a 10:1 split injection. The samples were separated on an Agilent DB-WAX capillary column (30 m length, 0.25 mm inner diameter, 0.25 µm phase thickness; Agilent, Santa Clara, CA, USA) with a gas chromatographic system. Quality control (QC) samples were set up at certain intervals to detect and evaluate the stability and reproducibility of the system. An Agilent 7890/5975C (Agilent, USA) was used for the MS analysis.

Shen J., Huang J., Huang Y., Chen Y., Li J., Luo P., Zhang Q., Qiu Y., Wang L., Jiang H., Ma S, & Chen X. (2022). Anlotinib suppresses lung adenocarcinoma growth via inhibiting FASN-mediated lipid metabolism. Annals of Translational Medicine, 10(24), 1337.

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Metabolomic Analysis of Retinal and RPE Cells

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Isolated mouse retina or confluent human fetal RPE cells were changed into pre-warmed Krebs-Ringer bicarbonate buffer (KRB) containing, depending on the experiment, [1,2] ¹³C glucose, U-¹³C glucose, or U-¹³C lactate (Sigma) as described elsewhere (Du et al., 2013a (link); Du et al., 2015 (link); Du et al., 2016b (link)). Both retinas and RPE cells were incubated for the specified time points. Metabolites from each time point were extracted and analyzed by gas chromatography mass spectrometry (GC-MS, Agilent 7890/5975C) as described in detail (Du et al., 2013a (link); Du et al., 2013b (link)).

Kanow M.A., Giarmarco M.M., Jankowski C.S., Tsantilas K., Engel A.L., Du J., Linton J.D., Farnsworth C.C., Sloat S.R., Rountree A., Sweet I.R., Lindsay K.J., Parker E.D., Brockerhoff S.E., Sadilek M., Chao J.R, & Hurley J.B. (2017). Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye. eLife, 6, e28899.

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Methylation Analysis of Polysaccharides

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Methylation analysis was conducted using the method of Ciucanu and Kerek with some modifications [46 (link)]. Polysaccharide samples (5 mg) were dried overnight at 40 °C in a vacuum oven and dissolved in DMSO (0.5 mL) by sonication at room temperature. Methyl iodide (0.3 mL) followed by finely powdered dry sodium hydroxide (20 mg) were added to a stirred solution of the polysaccharide dissolved in DMSO under nitrogen at room temperature. Then, the mixture was stirred for two hours. The methylated polysaccharides were extracted with dichloromethane. The dichloromethane phase was washed three times with 1 mL of water and dried with a stream of nitrogen. The methylated polysaccharide was reduced by NaBH₄ and then converted into partially methylated alditol acetates (PMAA) and analyzed by GC-MS (Agilent 7890/5975C, Agilent Technologies, Palo Alto, CA, USA) equipped with a HP-5 capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness).

Cai L., Wan D., Yi F, & Luan L. (2017). Purification, Preliminary Characterization and Hepatoprotective Effects of Polysaccharides from Dandelion Root. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(9), 1409.

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Extraction and Analysis of Hydrocarbons

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Hydrocarbons in liquid solutions were dissolved in dichloromethane, and all solutions were transferred into separatory funnel. Flasks were then washed twice by 20 ml of dichloromethane and then mixed with precious solutions. Hydrocarbons were then extracted using dichloromethane three times and all organic phase was collected. Dehydration of collected solution was performed by addition of excessive magnesium sulfate (MgSO₄). Dehydrated solutions were then concentrated by using rotary evaporator to a final volume of 10 ml. Extracted hydrocarbons were analyzed by GC-MS (Agilent 7890-5975c) equipped with a DB-5 capillary column (30 m × 250 μm × 0.25 μm, Agilent Technologies, U.S.A.). High purity helium (99.999%) was applied as the carrier gas with the speed of 1 ml/min. The temperature program was conducted as follows, 50–200 °C at 25 °C/min, 200–260 °C at 15 °C/min, and 260 °C for 10 min. Injector and transfer line temperatures were both set at 260 °C. Degradation efficiency was calculated by comparing peak areas of hydrocarbons.

Wang H., Wang B., Dong W, & Hu X. (2016). Co-acclimation of bacterial communities under stresses of hydrocarbons with different structures. Scientific Reports, 6, 34588.

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Metabolic Profiling via GC-MS

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An Agilent 7890/5975C gas chromatograph–mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) was used for acquisition of metabolic data. A Labconco CentriVap system (Labconco, Kansas City, MO, USA) was used to concentrate and dry the metabolite extracts. Ultrapure water was provided by a Milli-Q system (Millipore Corp, Millipore, MA, USA).
Acetonitrile used as the extraction solvent was from Merck (Fairfield, OH, USA). Methoxyamine, methyl-trimethylsilyl-trifluoroacetamide (MSTFA), urease, and pyridine were provided by Sigma-Aldrich (St. Louis, MO, USA). Standards for metabolite identification were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Li Q., Wei S., Wu D., Wen C, & Zhou J. (2018). Urinary Metabolomics Study of Patients with Gout Using Gas Chromatography-Mass Spectrometry. BioMed Research International, 2018, 3461572.

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Synthesis and Characterization of 4H-8-MQL

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8-MQL was provided from Alligator Reagent, which was further purified by vacuum distillation. Commercial 5 wt% Ru/Al₂O₃ catalyst was supplied by Shanxi Kaida Chemical Engineering Company Limited. Sinopharm Chemical Reagent Company Limited supplied 1,4-dioxane, ethanol and toluene. Energy Chemical Company provided N,N-diisopropylethylamine. All solvents and catalysts were used as received. Ultra-high purity of H₂ and Ar were provided by Sichuan Ally High-Tech Company. All liquid samples taken from reactors during experiments were regularly detected by Gas Chromatography and Mass Spectrometry (Agilent 7890/5975C, GCMS) to determine the composition. The samples diluted by hexane were carried by ultra-high purity He at a flow rate of 1.5 ml min⁻¹ into the chromatographic column (HP-5, 30 m × 320 μm × 0.25 μm). The injection temperature was 300 °C and split ratio was 1 : 100. The oven temperature started at 90 °C, then increased to 220 °C by 12 °C min⁻¹ subsequently and kept 220 °C for 1 min. Nuclear magnetic resonance (Bruker 400, NMR) was applied to determine the structure of 4H-8-MQL produced in 8-MQL hydrogenation.

Dong Y., Zhao H., Liu Z., Yang M., Zhang Z., Zhu T, & Cheng H. (2020). Understanding the mechanism of the competitive adsorption in 8-methylquinoline hydrogenation over a Ru catalyst. RSC Advances, 10(19), 11039-11045.

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Chemical Profiling of PM2.5 Particulates

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The chemical components of PM_2.5 and its extracts were analyzed by inductively coupled plasma-mass spectrometry (ELAN DRC II, PerkinElmer, America) and inductively coupled plasma atomic emission spectrometry (SPS-8000, Leeman, USA) for 23 types of metals, and gas chromatography-mass spectrometer (Agilent 7890/5975C, Agilent Technologies, USA) for 16 types of PAHs. The list of the chemical components is presented in Supplementary Table S1 (Supplementary material 2).

Wei H., Liang F., Meng G., Nie Z., Zhou R., Cheng W., Wu X., Feng Y, & Wang Y. (2016). Redox/methylation mediated abnormal DNA methylation as regulators of ambient fine particulate matter-induced neurodevelopment related impairment in human neuronal cells. Scientific Reports, 6, 33402.

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