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Potassium phosphate monobasic and dibasic

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Potassium phosphate monobasic and dibasic are inorganic salts commonly used in laboratory settings. Potassium phosphate monobasic (KH2PO4) is a crystalline solid, while potassium phosphate dibasic (K2HPO4) is a crystalline powder. These compounds are often used as buffers, pH regulators, and electrolytes in various laboratory procedures and applications.

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Lab products found in correlation

Hydrochloric acid (hcl), by Merck Group (4 mentions) Methanol, by Merck Group (3 mentions) Allopurinol, by Merck Group (3 mentions) Xanthine, by Merck Group (3 mentions) Xanthine oxidase, by Merck Group (3 mentions) Acetic acid, by Kanto Chemical (3 mentions) Sodium acetate, by Kanto Chemical (3 mentions) Sodium bicarbonate, by Thermo Fisher Scientific (3 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Acetic acid, by Merck Group (2 mentions) Potassium ferricyanide, by Merck Group (2 mentions) Tween 20, by Merck Group (2 mentions) Escherichia coli, by Merck Group (2 mentions) 1 1 diphenyl 2 picrylhydrazyl dpph, by Kanto Chemical (2 mentions) Staphylococcus aureus, by Merck Group (2 mentions) Bacillus subtilis, by Merck Group (2 mentions) Proteus mirabilis, by Merck Group (2 mentions) Dibutyl hydroxytoluene, by Kanto Chemical (2 mentions) Ethanol, by Junsei (2 mentions) Methanol, by Junsei (2 mentions)

19 protocols using potassium phosphate monobasic and dibasic

1

Characterization of IgG2 monoclonal antibody

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The mAb used in this study was an IgG2 (hereafter referred to as “mAb”) and was generously provided by Pfizer (Chesterfield, Missouri). Isoelectric point (pI) values for this mAb, determined by isoelectric focusing, ranged from 8.65 to 9.30.9 (link) Monobasic and dibasic potassium phosphate, potassium chloride, phosphoric acid, sodium azide, sodium acetate, acetic acid, guanidinium HCl (Gdn HCl), N-acetyl-L-tryptophanamide (NATA), and ribonuclease A (RNase A) were purchased from Sigma–Aldrich (St. Louis, Missouri). PS80, (containing low carbonyl and peroxide) was purchased from Thermo Scientific (Rockford, Illinois). Sucrose was obtained from Pfanstiehl Laboratories (Waukegan, Illinois). Distilled, deionized water was used throughout.
LIU L., BRAUN L.J., WANG W., RANDOLPH T.W, & CARPENTER J.F. (2014). Freezing-Induced Perturbation of Tertiary Structure of a Monoclonal Antibody. Journal of pharmaceutical sciences, 103(7), 1979-1986.
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2

Cytochrome c Spectroscopy in Deep Eutectic Solvents

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Cytochrome c from the equine heart (>95% protein content), urea, ethylammonium chloride, monobasic and dibasic potassium phosphate, sodium dithionite, and D2O were purchased from Sigma-Aldrich and used as received. The pH 7 potassium phosphate buffer solutions were prepared in water and D2O. Carbon monoxide cytochrome c was prepared by reduction with sodium dithionite solution, followed by purging with CO gas for ∼1 h. The detailed preparation of urea-ethylammonium chloride DES was described elsewhere.20 (link) In short, EAC was mixed with urea (molar ratio of 1:1.5) and incubated at 80 °C for ∼1 h until a colorless liquid was formed. The resulting DES was mixed with phosphate buffer to achieve the desired concentrations. The cytochrome c concentration for FTIR and 2D-IR spectroscopy was 16 mM for buffer, EAC/buffer, and urea/buffer solutions and 11 mM for the DES/buffer solution.
Osawa K., Kossowska D., Park K., Kwak K, & Cho M. (2019). Two-dimensional infrared spectroscopic study of cytochrome c peroxidase activity in deep eutectic solvent. Structural Dynamics, 6(6), 064703.
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3

HPLC Analysis of Pharmacological Compounds

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All HPLC analytical solvents (methanol and acetonitrile) were obtained from Sigma Aldrich, Co. (Gillingham, UK). High purity (>98%) salicylic acid, magnesium chloride (MgCl2), testosterone, phenacetin, monobasic and dibasic potassium phosphate, 85% purity phosphoric acid and Tris-HCL were obtained from Sigma Aldrich, Co. (Gillingham, UK). Finally, HPLC Uridine 5′-diphosphogluouronic acid, HPLC alamethicin from Trichoderma viride, and testosterone glucuronide were procured from Carbosynth limited (Compton, UK).
Salhab H., Naughton D.P, & Barker J. (2021). Potential Assessment of UGT2B17 Inhibition by Salicylic Acid in Human Supersomes In Vitro. Molecules, 26(15), 4410.
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4

Lipase activity assay protocol

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Monobasic and dibasic potassium phosphate, magnesium sulfate, p-nitrophenyl butyrate, 4-(1,1,3,3-tetramethylbutyl) phenyl-polyethylene glycol (Triton X-100), p-nitrophenol, 2-methyl-2-butanol, yeast extract, bacteriological peptone, glucose, agar, olive oil and Tween 80 were purchased from Sigma Aldrich-Fluka (Toluca, Mexico) and local suppliers. The commercial Candida antarctica B lipase (CaLB) from Novozyme was used to validate the first measurements in the SIA system.
Pliego J., Mateos J.C., Rodriguez J., Valero F., Baeza M., Femat R., Camacho R., Sandoval G, & Herrera-López E.J. (2015). Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate. Sensors (Basel, Switzerland), 15(2), 2798-2811.
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5

Peroxidase Assay with Alcoholic Solvents

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The salt-free, lyophilized powder of HRP isozyme C type VI-A (950–2000 units per mg solid), monobasic and dibasic potassium phosphate (≥98%), potassium ferricyanide (≥99.0%), and ABTS (≥98%) were purchased from Sigma Aldrich (St. Louis, MO). Hydrogen peroxide (30% w/w solution) was purchased from Anachemia (Que, Canada). Pure 200 proof ethanol was purchased from KOPTEC (PA, US) and glycerol was purchased from Mallinckrodt Chemicals (Wilkes-Barre, PA). Other alcohols including propan-2-ol, methanol, 2-mercaptoethan-1-ol, 2-chloroethan-1-ol, 2,2-dichloroethan-1-ol, 2,2,2-trichloroethan-1-ol, 2,2,2-trifluoroethan-1-ol, and ethylene glycol were purchased from Sigma Aldrich (St. Louis, MO).
Ravanfar R, & Abbaspourrad A. (2021). Monitoring the heme iron state in horseradish peroxidase to detect ultratrace amounts of hydrogen peroxide in alcohols. RSC Advances, 11(17), 9901-9910.
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6

Comprehensive Phytochemical Characterization

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The following reagents were employed: acetone was acquired from Labsynth (Diadema, SP, Brazil), hexane, ethanol, methanol and methyl tert-butyl ether (MTBE) were acquired from Merck (Darmstadt, Germany), vanillin and sulfuric acid (H2SO4) were obtained from Dinâmica Química Contemporânea (Diadema, SP, Brazil), and the Folin–Ciocalteu reagent, sodium carbonate Na₂CO₃, dithiothreitol (DTT), metaphosphoric acid, potassium chloride (KCl), Tris (hydroxymethyl) aminomethane, potassium peroxydisulfate, 2,20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), monobasic and dibasic potassium phosphate, fluorescein sodium salt, 2,20-azobis(2-methylpropionamidine) dihydrochloride (AAPH), sodium hypochlorite solution (NaOCl) and rhodamine 123 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid, ascorbic acid, lutein, zeaxanthin, β-cryptoxanthin, α-carotene β-carotene, catechin, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-car- boxylic acid (Trolox) were used as standards and purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was obtained from a Millipore Milli-Q System (Merck Millipore, Steinfurt, Germany).
Taver I.B., Spricigo P.C., Neto H.B., de Alencar S.M., Massarioli A.P, & Jacomino A.P. (2022). Bioactive Compounds and In Vitro Antioxidant Capacity of Cambuci and Uvaia: An Extensive Description of Little-Known Fruits from the Myrtaceae Family with High Consumption Potential. Foods, 11(17), 2612.
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7

Optimized Insulin Immunoassay Protocol

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Dibasic and monobasic potassium phosphate, sodium carbonate and bicarbonate, bovine insulin, and Tween-20 were purchased from Sigma Aldrich (Saint Louis, MO). Ethylenediamine tetraacetic acid (EDTA), bovine serum albumin (BSA), sodium hydroxide, and ammonium hydroxide were obtained from EMD Chemicals (San Diego, CA). Nitric acid, hydrofluoric acid, and hydrogen peroxide were obtained from Macron Fine Chemicals, and sulfuric acid was from J.T. Baker (Center Valley, PA). For all solutions and sample preparation, ultrapure deionized water was used (NANOpure Diamond TM deionization system, Barnstead International, Inc., Dubuque, IA).
The following reagents were utilized for the insulin immunoassay. Monoclonal antibody (Ab) for the C-terminal of human insulin was obtained from Meridian Life Science, Inc. (Torrance, CA). For production of Cy5-labeled insulin (Ins*) Cy5 monofunctional N-hydroxysuccinimide ester was obtained from GE Healthcare Bio-Sciences (Piscataway, NJ). The methodology for labeling bovine insulin has been described previously [7 (link)] and was performed according to the manufacturer’s guidelines.
Mukhitov N., Yi L., Schrell A.M, & Roper M.G. (2014). Optimization of A Microfluidic Electrophoretic Immunoassay Using a Peltier Cooler. Journal of chromatography. A, 1367, 154-160.
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8

Enzyme Assay Protocol Chemicals

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The following chemicals were purchased from Sigma (Germany): dibasic and monobasic potassium phosphate; 1-chloro-2,4-dinitrobenzene; L-glutathione (reduced form); 5,5-dithiobis(2-nitrobenzoic acid); sodium hydrogen carbonate; acetylthiocholine iodide. BCA Protein Assay Reagents A and B, cadmium chloride, and potassium dichromate were purchased from Pierce (U.S.A.). All chemicals were of the highest commercially available grade, typically 99% or higher.
Jemec A., Škufca D., Prevorčnik S., Fišer Ž, & Zidar P. (2017). Comparative study of acetylcholinesterase and glutathione S-transferase activities of closely related cave and surface Asellus aquaticus (Isopoda: Crustacea). PLoS ONE, 12(5), e0176746.
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9

Antioxidant and Antibacterial Evaluation

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Methanol, hexane, chloroform, ethyl acetate and ethanol were purchased from Junsei Chemical Co., Ltd., Tokyo, Japan. Potassium phosphate monobasic and dibasic, xanthine, xanthine oxidase, allopurinol, and hydrochloric acid were obtained from Sigma-Aldrich Corp., St. Louis, MO, USA. Reagents including 1,1-diphenyl-2-picrylhydrazyl (DPPH), sodium acetate, acetic acid, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium peroxodisulfate, and dibutyl hydroxytoluene (BHT) were supplied by Kanto Chemical Co. Inc., Tokyo, Japan. Four bacteria including Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Proteus mirabilis were provided by Sigma-Aldrich Corp., St. Louis, MO USA. All chemicals used were of analytical grade.
Quy T.N, & Xuan T.D. (2019). Xanthine Oxidase Inhibitory Potential, Antioxidant and Antibacterial Activities of Cordyceps militaris (L.) Link Fruiting Body. Medicines, 6(1), 20.
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10

Equine Skeletal Muscle Mb Crosslinking

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Holo-myoglobin from equine skeletal muscle (Mb), potassium phosphate monobasic and dibasic, Tris base, D-(+)-raffinose pentahydrate, guanidine hydrochloride (Gdn) and anhydrous dimethyl sulfoxide (DMSO) were purchased from Sigma Aldrich (St. Louis, MO). The heterobifunctional crosslinker succinimidyl 4,4’-azipentanoate (SDA) was obtained from Thermo Scientific (Rockford, IL). Trypsin was obtained from Promega (Madison, WI) and mass spectrometry-grade water, acetonitrile and formic acid from Fisher Scientific (Fair Lawn, NJ).
Iyer L.K., Moorthy B.S, & Topp E.M. (2015). Photolytic Crosslinking to Probe Protein-Protein and Protein-Matrix Interactions In Lyophilized Powders. Molecular pharmaceutics, 12(9), 3237-3249.
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