Sodium butyrate (SB; Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) was dissolved in phosphate-buffered saline (PBS; 0.14 M NaCl, 2.7 mM KCl, 10 mM Na2HPO4 and 1.8 mM KH2PO4). LPS obtained from Escherichia coli with serotype 055:B5 (Sigma-Aldrich, St. Louis, MO, USA), a rat/human CRF (Peptide Institute, Inc., Asagi, Japan), NG-nitro-L-arginine methyl ester (L-NAME), naloxone hydrochloride and domperidone (Fujifilm Wako Pure Chemical) were dissolved in normal saline. Compound C (dorsomorphin; LC Laboratories, Inc., Woburn, MA, USA), GW9662 (Focus Biomolecules, Plymouth Meeting, PA, USA) and sulpiride (Fujifilm Wako Pure Chemical) were dissolved in dimethyl sulfoxide (Fujifilm Wako Pure Chemical). The doses and routes of administration of the chemicals were determined according to previous publications5 (link),6 (link),17 (link)–20 (link).
Rat human crf
Manufactured by Peptide Institute
Sourced in United States
Rat/human CRF is a laboratory product used for research purposes. It is a peptide that functions as a corticotropin-releasing factor, a hormone involved in the stress response. This product can be used in various experimental settings to study the physiological and biochemical processes related to stress and the hypothalamic-pituitary-adrenal axis.
Automatically generated - may contain errors
Lab products found in correlation
Ng nitro l arginine methyl ester l name, by Fujifilm (2 mentions)
Sulpiride, by Fujifilm (2 mentions)
Naloxone hydrochloride, by Fujifilm (2 mentions)
Domperidone, by Fujifilm (2 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
Astressin 2 b, by Merck Group (1 mentions)
Bicuculline, by Merck Group (1 mentions)
2 protocols using rat human crf
1
Solubilizing Reagents for Biochemical Assays
2
DHEA-S and CRF Receptor Antagonism
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DHEA-S sodium hydrate (Tokyo Chemical Industry, Tokyo, Japan), LPS obtained from Escherichia coli with the serotype 055:B5 (Sigma-Aldrich, St. Louis, MO, USA), rat/human CRF (Peptide Institute Inc., Asagi, Japan), N G -nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthesis inhibitor, naloxone hydrochloride, an opioid receptor antagonist and domperidone (Wako Pure Chemical Industries, Osaka, Japan), a peripheral dopamine D2 receptor antagonist were dissolved in normal saline. Sulpiride (Wako Pure Chemical Industries), a dopamine D2 receptor antagonist and bicuculline (Sigma-Aldrich), a γ-aminobutyric acid (GABA)A receptor antagonist was dissolved in saline containing 10 % dimethyl sulfoxide (DMSO). Astressin2-B, a selective CRF receptor subtype 2 (CRF2) antagonist (Sigma-Aldrich) was dissolved in double-distilled water. The doses of the chemicals were determined according to the previous reports (Nozu et al., 2017a (Nozu et al., , 2019;; Nozu et al., 2017b; Samardzic et al., 2017) .
The volume of injection was 0.2 ml/rat. DHEA-S, L-NAME, CRF or Astressin2-B was intraperitoneally injected. Other chemicals were administered via subcutaneous route.
The volume of injection was 0.2 ml/rat. DHEA-S, L-NAME, CRF or Astressin2-B was intraperitoneally injected. Other chemicals were administered via subcutaneous route.
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