Quercetin (chemical purity >98.5%) was purchased from National Institute for Food and Drug Control (Beijing, China). UDPGA, alamethicin and β-D-glucuronidase were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The pGEM-T plasmid was purchased from Promega (Madison, WI, USA). The pECFP-N1 plasmid and pEYFP-N1 plasmid were purchased from Clontech Laboratories, Inc (Palo Alto, CA, USA). Restriction endonucleases, DNA molecular marker, PrimeScript RT reagent kit and T4 ligase were obtained from TaKaRa Bio Inc. (Dalian, Liaoning, China). Cellfectin II reagent, pFastBac1 vector, E. coli DH10Bac cells, Sf900II SFM, and Gibco fetal bovine serum were purchased from Invitrogen Corp. (Carlsbad, CA, USA). Spodoptera frugiperda Sf9 insect cells were obtained from the China Center for Type Culture Collection (Wuhan, China). Rabbit Anti-UGT1A Polyclonal antibody was obtained from Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (Beijing, China). The anti-HA beads were purchased from Roche Applied Science (Indianapolis, IN, USA). SuperSignal West Pico was obtained from Pierce Chem Co. (Rockford, IL, USA) and X-ray film obtained from Kodak (Rochester, NY, USA).
Gibco fetal bovine serum
Manufactured by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom
Gibco™ fetal bovine serum (FBS) is a widely used cell culture supplement derived from the blood of fetal bovine calves. It provides a complex mixture of growth factors, hormones, and other nutrients essential for the in vitro cultivation of a variety of cell types.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (17 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (10 mentions)
Penicillin streptomycin, by Merck Group (7 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (7 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (6 mentions)
Gibco dmem, by Thermo Fisher Scientific (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (5 mentions)
Gibco rpmi 1640 medium, by Thermo Fisher Scientific (5 mentions)
Streptomycin, by Thermo Fisher Scientific (5 mentions)
L glutamine, by Merck Group (4 mentions)
Hyclone dulbecco s modified eagle medium ham s f12 dmem f12 1 1, by Thermo Fisher Scientific (4 mentions)
Rosiglitazone, by AK Scientific (4 mentions)
Penicillin, by Merck Group (4 mentions)
Dmem f12, by Thermo Fisher Scientific (4 mentions)
Trypsin edta, by Thermo Fisher Scientific (4 mentions)
Gibco antibiotic antimycotic, by Thermo Fisher Scientific (3 mentions)
Protease and phosphatase, by Cell Signaling Technology (3 mentions)
Tgx protein gels, by Bio-Rad (3 mentions)
Ripa buffer, by Merck Group (3 mentions)
Streptomycin, by Merck Group (3 mentions)
101 protocols using gibco fetal bovine serum
1
Quercetin Glucuronidation Activity Assay
2
Recombinant Protein Production in Insect Cells
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Cellfectin II reagent, pFastBac1 vector, E. coli DH10Bac cells, Sf900II SFM, and Gibco fetal bovine serum were purchased from Invitrogen (Carlsbad, CA, USA). Spodoptera frugiperda Sf9 insect cells were obtained from the China Center for Type Culture Collection (Wuhan, China). Rabbit anti-human UGT2B7 polyclonal antibodies were purchased from ProteinTech Group, Inc. (Chicago, USA). Rabbit anti-UGT1A Polyclonal antibodies were obtained Institute of Genetics and Developmental Biology Chinese Academy of Sciences (Beijing, China). Rabbit anti-human UGT2B7 polyclonal antibodies were purchased from ProteinTech Group, Inc. (Chicago, USA).
Zidovudine and quercetin (chemical purity >98.5%) were purchased from National Institute for Food and Drug Control (Beijing, China). Zidovudine O-glucuronide was purchased from Toronto Research Chemicals (Toronto, Canada). Uridine 5-diphosphoglucuronic acid (UDPGA), alamethicin, paraformaldehyde (PFA) and deoxycholic acid were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Nonidet P-40 and Tween-20 were purchased from Amresco Inc. (Solon, OH, USA). Tris-HCl, NaCl, EDTA, MgCl2, perchloric acid and KH2PO4 were purchased from Sinopharm Chemical Regent Co. (Beijing, China). Chromatographic grade methanol was purchased from Tedia, Co. (Fairfield, OH, USA).
Zidovudine and quercetin (chemical purity >98.5%) were purchased from National Institute for Food and Drug Control (Beijing, China). Zidovudine O-glucuronide was purchased from Toronto Research Chemicals (Toronto, Canada). Uridine 5-diphosphoglucuronic acid (UDPGA), alamethicin, paraformaldehyde (PFA) and deoxycholic acid were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Nonidet P-40 and Tween-20 were purchased from Amresco Inc. (Solon, OH, USA). Tris-HCl, NaCl, EDTA, MgCl2, perchloric acid and KH2PO4 were purchased from Sinopharm Chemical Regent Co. (Beijing, China). Chromatographic grade methanol was purchased from Tedia, Co. (Fairfield, OH, USA).
3
Hormone Reagents for Cell Culture
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The following chemicals and reagents were obtained from the indicated sources: GIBCO fetal bovine serum (Invitrogen, Carlsbad, CA); testosterone, DHT, penicillin–streptomycin, NKB, and prolactin (Sigma-Aldrich Co., St. Louis, MO); and DynA (Cayman Chemical, Ann Arbor, MI).
4
Site-Directed Mutagenesis Protocol
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A QuikChange II Site-Directed Mutagenesis Kit, Lipofectamine 2000, Dulbecco’s modified Eagle medium (DMEM) and GIBCO fetal bovine serum were obtained from Invitrogen. Carbon-coated grids were obtained from Quantifoil. Mouse anti-M1 monoclonal antibody and rabbit anti-NP or anti-HA polyclonal antibody were prepared by standard procedures. The origin of other materials was described in the previous study (Zhang et al., 2012 (link)).
5
Culturing Caco-2 and Detroit 562 Cell Lines
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Caco-2 cells (Riken Cell Bank), a human colon carcinoma cell line, were cultured in minimum essential media (Life Technologies) supplemented with 20% heat-inactivated fetal bovine serum (SAFC Biosciences) (Matsumura CM). Detroit 562 cells (ATCC CCL-138), from a human pharyngeal cell line, were obtained from American Type Culture Collection and maintained in minimum essential medium-α (α-MEM, Wako) supplemented with 10% heat-inactivated Gibco fetal bovine serum (Life Technologies).
6
Serological Diagnosis of Rift Valley Fever
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For the serological diagnosis of RVF, each participant received a panel of 15 ruminant sera composed of 5 negative and 10 positive samples. The positive samples consisted of sera from domestic and wild ruminants: five samples were from RVFV vaccinated sheep (n = 4) and goats (n = 1) seropositive for IgG. Animals were vaccinated with the formalin inactivated Rift Valley fever virus commercially available from the Onderstepoort Biological Product (OBP, Onderstepoort, South Africa). Five samples were from 5 springboks (Antidorcas marsupialis) found RVF seropositive for both IgG and IgM according with the results of ELISAs ID Screen® Rift Valley Fever Competition Multi-species and ID Screen® Rift Valley Fever IgM Capture (IDvet, Grabels, France) for IgG and IgM detection respectively. Negative samples were prepared from a single bovine RVF seronegative serum (Gibco® Fetal Bovine Serum, Life Technologies, Carlsbad, USA). To exclude the presence of any infectious viral particle, the samples were tested by RT-PCR [9 (link)] and heated at 56°C for three hours. The inactivation process was assessed as described above. Each set of samples was evaluated for homogeneity by testing 5 replicates with the above ELISAs. Stability was evaluated with the ELISA tests cited above by using the number of samples and the time intervals t0, t1 (72 hours) t2 (7 days).
7
Umbilical Cord Mesenchymal Stromal Cell Isolation
8
Immune Cell Activation Assay
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Histopaque-1077 and sodium azide were purchased from Sigma-Aldrich (St. Louis, MO); Dulbecco’s minimal Eagle’s medium (DMEM), Dulbecco's phosphate-buffered saline (DPBS), Gibco fetal bovine serum (FBS), and Gibco penicillin-streptomycin mixture (Pen Strep) were purchased from Life Technologies (Grand Island, NY). Bovine TSH and Akt inhibitor IV (AKTi) were purchased from Calbiochem EMD Biosciences (La Jolla, CA). Soluble CD40L (MegaCD40L) was purchased from Enzo Life Sciences (Farmingdale, NY). The nuclear factor (NF)-κB inhibitor MG132 was provided by Cayman Chemical (Ann Arbor, MI).
9
Culturing Cardiomyocytes and HEK Cells
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HL1 cardiomyocytes were cultured in Claycomb Medium (51800C, Sigma-Aldrich) supplemented with 10% fetal bovine serum (F2442, Sigma-Aldrich), Penicillin/Streptomycin 100 U/mL: 100 μg/ml (P4333, Sigma-Aldrich), 2 mM L-Glutamine (G7513, Sigma-Aldrich), and 0.1 mM Norepinephrine [(±)-Arterenol] (A0937, Sigma-Aldrich) in a humidified 5% CO2, 95% O2 incubator at 37°C.
HEK293 cells and HEK293T packaging cells were cultured in Gibco™ DMEM, high glucose, GlutaMAX™ (31966-021, Life Technologies™) supplemented with 10% Gibco™ Fetal Bovine Serum (10270-106, Life Technologies™) and 1% Penicillin-Streptomycin (10,000 U/mL,15140122, Gibco™, Life Technologies™) in a humidified 5% CO2, 95% O2 incubator at 37 °C.
Cell concentration and viability were assessed using Trypan Blue Stain, 0.4% with LUNA-II™ Automated Cell Counter (Logos Biosystems).
HEK293 cells and HEK293T packaging cells were cultured in Gibco™ DMEM, high glucose, GlutaMAX™ (31966-021, Life Technologies™) supplemented with 10% Gibco™ Fetal Bovine Serum (10270-106, Life Technologies™) and 1% Penicillin-Streptomycin (10,000 U/mL,15140122, Gibco™, Life Technologies™) in a humidified 5% CO2, 95% O2 incubator at 37 °C.
Cell concentration and viability were assessed using Trypan Blue Stain, 0.4% with LUNA-II™ Automated Cell Counter (Logos Biosystems).
10
Culturing H9c2 Rat Cardiomyocytes
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H9c2 cells (Rattus norvegicus heart myoblast cell line) were purchased from ATCC (Burlington, ON, Canada) and cultured with Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Mississauga, ON, Canada) supplemented with 10% GIBCO fetal bovine serum (FBS; Thermo Fisher Scientific) and 100 U/mL penicillin-streptomycin (Thermo Fisher Scientific), at conditions of 37°C and 5% CO2 until 80%–90% confluence for subculturing.
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