Antigen-antibody interactions were analyzed using an SPRi label-free interaction analysis platform, OpenPlex (HORIBA). The antigen, CD20 (Sino Biological, 11,007-H34E-B), was immobilized on the surface of a CS-HD biochip (HORIBA) with the amine coupling method. Neutral and acid-treated rituximab solutions were diluted into 20 mM citrate-phosphate buffer at pH 7.0 (running buffer) to 5 nM. The association was recoded at a rate of 50 μl/min for 180 s. The dissociation was measured by flowing running buffer alone for 540 s, followed by injecting 100 mM Gly-HCl at pH 3.2 for 60 s to regenerate the biochip. The experiments were performed at room temperature, and the reflectivity variation (%) in ligand spots was calculated by subtracting the signals of untreated areas on the chip surface. The collected data were analyzed using the ScrubberGen software (HORIBA).
Scrubbergen software
Manufactured by Horiba
Sourced in France
ScrubberGen is a software tool developed by Horiba for analyzing and optimizing the performance of gas scrubber systems. The software provides functionalities for modeling, simulating, and evaluating the efficiency of gas scrubbers used in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using scrubbergen software
1
SPR Analysis of Rituximab Binding
2
SPRi Analysis of Protein Binding
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Surface plasmon resonance imaging (SPRi) analyses were performed using an SPRi system OpenPlex (HORIBA France, Palaiseau, France) according to a previous report.18 Briefly, 10 µM protein samples and normal human IgG (Sigma‐Aldrich) in PBS were spotted onto an SPRi‐Biochip (HORIBA France) at 10 nl/spot using a DNA Array Spotter (HORIBA Ltd., Kyoto, Japan). The reflectivity of each spot in response to 2.4 µM analyte samples diluted in running buffer (PBS with 0.2% BSA and 0.02% Tween20) was recorded, and the percentage change in reflectivity (%ΔR) was calculated by subtracting the background signal. Data were processed using ScrubberGen software (HORIBA France).
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