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Gtmac

Manufactured by Merck Group
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GTMAC is a laboratory equipment product manufactured by the Merck Group. It serves as a core function for specific laboratory applications. The description is presented in a factual and unbiased manner, without extrapolation on its intended use.

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Lab products found in correlation

Span 80, by Merck Group (1 mentions) Simplicity system, by Merck Group (1 mentions) Ch3cooh, by Chempur (1 mentions) Span 40, by Merck Group (1 mentions) Gelred, by Biotium (1 mentions) Chitosan, by Merck Group (1 mentions) Sodium nitrite, by Merck Group (1 mentions) Vybrant mtt cell proliferation assay kit mtt, by Thermo Fisher Scientific (1 mentions) Glycidyltrimethylammonium chloride, by Merck Group (1 mentions) Hydroxypropyl cellulose, by Merck Group (1 mentions) Sodium p styrene sulfonate, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Ch3coona, by Merck Group (1 mentions) K2cro4, by Merck Group (1 mentions) Ethanol c2h6o, by Merck Group (1 mentions) Glutaraldehyde c5h8o2, by Merck Group (1 mentions) Cetyltrimethylammonium bromide, by Merck Group (1 mentions)

Spelling variants of this product

Glycidyltrimethylammonium chloride (GTMAC) (8 citations)

7 protocols using gtmac

1

Fabrication of PET Spunbond Fabric Composites

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Polyethylene terephthalate (PET) spunbond fabric (basis mass: 15 g·m−2, thickness: 90 μm, fibre diameter: 300–500 μm) was acquired from Freudenberg Far Eastern Spunweb Co., Ltd. (Taoyuan, Taiwan). PVA (Mw: 75,000 to 180,000 g·mol−1) and chitosans (average Mw: 340 kD; deacetylation degree, DD: 90–95%) were obtained from Charming & Beauty Co., Ltd. (Taipei, Taiwan). GTMAC and all other unmentioned chemicals used in the experiments were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Wu J.Y., Wang C.Y., Chen K.H., Lai Y.R., Chiu C.Y., Lee H.C, & Chang Y.K. (2022). Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy. Membranes, 12(3), 332.
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2

Synthesis and Characterization of Quaternized Chitosan

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Quaternized CS, i.e., N-[(2-hydroxy-3-trimethylammonium) propyl]chitosan salt (HTCS), was synthesized according to the procedure described by Heydari et al. [32 (link)]. In short, 5.0 g of CS (30.3 mmol of glucosamine) was dispersed in 100 mL of water and stirred for 30 min at 80 °C. Then, 32.5 mL (242.4 mmol) of glycidyltrimethyl ammonium chloride (GTMAC, ≥90%, Sigma-Aldrich, Saint Louis, MO, USA) was added in six equal volumes in 1 h intervals, and the reaction proceeded at 80 °C for 16 h. The resulting solution was diluted with water and precipitated in an excess of cold acetone. The polymer was extracted using a Soxhlet extractor in ethanol and acetone and then dried in the oven at 50 °C for 24 h. The degree of quaternization (DQ) was 116 %, determined by 1H NMR, and the weight-average molar mass was 130,000 g·mol−1, measured following the protocols recommended by us previously [32 (link)].
Dehshahri A., Khalvati B., Taheri Z., Safari F., Mohammadinejad R, & Heydari A. (2022). Interleukin-12 Plasmid DNA Delivery by N-[(2-Hydroxy-3-trimethylammonium)propyl]chitosan-Based Nanoparticles. Polymers, 14(11), 2176.
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3

Chitosan-based Biomaterial Synthesis

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Chitosan (CHIT, low molecular weight, 75–85% deacetylated, Sigma-Aldrich), genipin (98%, Challenge Bioproducts), sorbitan monopalmitate (Span®40, Sigma-Aldrich), sorbitan monooleate (Span®80, Sigma-Aldrich), glycidyltrimethylammonium chloride (GTMAC, ≥ 90%, Sigma-Aldrich), cyclohexane (p.a, POCh), glacial acetic acid (CH3COOH, 99,85% pure p.a., CHEMPUR), acetone (POCh), methanol (POCh) and sodium chloride (NaCl, POCh) were used as received. Doubly distilled water was deionized using the Millipore Simplicity system.
Ciejka J., Wolski K., Nowakowska M., Pyrc K, & Szczubiałka K. (2017). Biopolymeric nano/microspheres for selective and reversible adsorption of coronaviruses. Materials Science & Engineering. C, Materials for Biological Applications, 76, 735-742.
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4

Chitosan-Based Nanocarrier for Targeted Delivery

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Chitosan, GTMAC (purity ≥ 90%), TPP (purity ≥ 98%), sodium nitrite (purity ≥ 97%), lipopolysaccharides (LPS) from Salmonella enteric serotype typhimurium (purity ≥ 99%) were purchased from Sigma-Aldrich (St. Louis, USA). Chitosan was purified before its subsequent application, and its molecular weight was tailored by depolymerization using sodium nitrite following a reported method [34 (link)]. Viscosity-average molecular weight of the resultant Chitosan was measured as 1.8×104 Da using a 0.5 M CH3COOH/0.2 M CH3COONa solvent system [35 ]. Oligofectamine (OF), 4′,6-diamidino-2-phenyl-indole dihydrochloride (DAPI), FITC fluorescently tagged siRNA (FITC-siRNA) and Vybrant® MTT cell proliferation assay kit (MTT) were obtained from Invitrogen (Eugene, USA). GelRed was from Biotium (Hayward, USA). siTNF was purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and its sequences were CGUCGUAGCAAACCACCAATT (sense strand) and UUGGUGGUUUGCUACGACGTG (anti-sense strand). All commercial products were used without further purification unless otherwise stated. Deionized water was obtained using a Milli-Q water purification system.
Xiao B., Ma P., Ma L., Chen Q., Si X., Walter L, & Merlin D. (2016). Effects of tripolyphosphate on cellular uptake and RNA interference efficiency of chitosan-based nanoparticles in Raw 264.7 macrophages. Journal of colloid and interface science, 490, 520-528.
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5

Hydrogel Synthesis Using Curdlan and HPC

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Curdlan, CUR (from Alcaligenes faecalis), Hydroxypropyl cellulose, HPC (average Mw ~80,000, average Mn ~10,000), Glycidyltrimethylammonium chloride, GTMAC (≥90%), sodium p-styrenesulfonate, SSS (90%) were purchased from Sigma-Aldrich (Poznan, Poland). Spectroscopic grade methanol and oleic acid (p.a.) were purchased from POCH (Gliwice, Poland).
Lachowicz D., Kaczyńska A., Bodzon-Kulakowska A., Karewicz A., Wirecka R., Szuwarzyński M, & Zapotoczny S. (2020). Coacervate Thermoresponsive Polysaccharide Nanoparticles as Delivery System for Piroxicam. International Journal of Molecular Sciences, 21(24), 9664.
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6

Chitosan-PVA Hydrogel for Biomedical Applications

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Medium weight chitosan (Sigma Aldrich), PVA (87%–89% hydrolyzed, Sigma Aldrich), glycidyl trimethyl ammonium chloride (GTMAC, Sigma Aldrich), acetic acid (CH3COOH, Riedel-de Haen), sodium acetate (CH3COONa, Merck), sodium hydroxide (NaOH, Merck), hydrochloric acid (HCl, 37%, Merck), perchloric acid (HClO4, Isolab), potassium chromate (K2CrO4, Merck), silver nitrate (AgNO3, Sigma Aldrich), ethanol (C2H6O, Sigma Aldrich), glutaraldehyde (C5H8O2, 25%, Sigma Aldrich), phosphate buffered saline tablet (PBS, Sigma Aldrich), and C. officinalis flowers were commercially supplied and used without further purification.
EREN BELGİN E, & DELİBALTA C.G. (2023). Quaternized chitosan/PVA/natural bioactive agent electrospun wound scaffolds: production, characterization, and investigation of release kinetics. Turkish Journal of Chemistry, 47(6), 1529-1539.
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7

Chitosan-Based Antiviral Formulation

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Chitosan [degree of deacetylation
(DDA) ≥75%; purity: 99%; CAS no. 9012-76-4] was purchased from
TCI (Japan). AETMAC, GTMAC, and ammonium persulfate were purchased
from Sigma-Aldrich (USA). Sodium hydrogen carbonate (NaHCO3) and benzaldehyde (C7H6O) were obtained from
Merck (Germany). Cetyl trimethyl ammonium bromide, calcium chloride,
magnesium sulphate, lecithin, peptone, Tween-80, and sodium thiosulphate
were purchased from Sigma-Aldrich (USA). Bacteriophage Phi-X174 (cat.
no. 124425) and its corresponding host Escherichia
coli-C (cat. no. 124400) were purchased from Carolina
Biological (Burlington, USA). Bacteriophage Phi-6 (cat. no. 21518)
and MS2 (cat. no. 13767) along with their corresponding host organisms Pseudomonas syringae (cat. no. 21482) and E. coli (Migula 1895, cat. no. 5695) (E. coli-M) were purchased from DSMZ-German Collection
of Microorganisms and Cell Cultures (Braunschweig, Germany). Luria-Bertini
(LB) broth was purchased from Condalab (Madrid, Spain).
Teotia A., Laurén I., Borandeh S, & Seppälä J. (2023). Quaternized Chitosan Derivatives as Viable Antiviral Agents: Structure–Activity Correlations and Mechanisms of Action. ACS Applied Materials & Interfaces, 15(15), 18707-18719.
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