Rhodamine dhpe

Manufactured by Thermo Fisher Scientific

Sourced in United States

Rhodamine-DHPE is a fluorescent lipid probe used in cell biology and biochemistry research. It is a derivative of rhodamine dye, which is conjugated to the phospholipid dipalmitoyl phosphatidylethanolamine (DHPE). Rhodamine-DHPE is commonly used to label and visualize cellular membranes and lipid bilayers.

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Lab products found in correlation

Nbd dhpe, by Thermo Fisher Scientific (2 mentions) Pi 3 p, by Echelon Biosciences (1 mentions) Marina blue dhpe, by Thermo Fisher Scientific (1 mentions) Texas red dhpe, by Thermo Fisher Scientific (1 mentions) Biotinylated r phycoerythrin, by Thermo Fisher Scientific (1 mentions) Ergosterol, by Merck Group (1 mentions)

2 protocols using rhodamine dhpe

Purification of Vacuolar Trafficking Proteins

Cited 3 times

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Lipids were obtained from Avanti Polar Lipids (Alabaster, AL), except for ergosterol which was from Sigma-Aldrich (St. Louis, MO), PI(3)P was from Echelon Biosciences (Salt Lake City, UT), and the fluorescent lipids (Marina-Blue-DHPE, NBD-DHPE, Rhodamine-DHPE, Oregon-Green-488-DHPE, Texas-Red-DHPE) were from Life Technologies (Carlsbad, CA). Biotinylated R-phycoerythrin was purchased from Life Technologies, Cy5-derivatized streptavidin from KPL (Gaithersburg, MD), and unlabeled streptavidin from Thermo Scientific (Waltham, MA). Sec18p (Haas and Wickner, 1996 (link)), Sec17p (Schwartz and Merz, 2009 (link)), Ypt7p (Zick and Wickner, 2013 (link)), HOPS (Zick and Wickner, 2013 (link)), and vacuolar SNARE proteins (Mima et al., 2008 (link); Schwartz and Merz, 2009 (link); Zucchi and Zick, 2011 (link)) were purified as described. Vti1p and Nyv1p were exchanged into octylglucoside buffer as described (Zucchi and Zick, 2011 (link)). Soluble Nyv1p (sR) was purified as GST-TEVsite-Nyv1(Δtm) as described (Thorngren et al., 2004 (link)), and cleaved with TEV protease prior to use. GST-His6-3Csite-Nyv1p was purified as described (Izawa et al., 2012 (link)).

Zick M, & Wickner W.T. (2014). A distinct tethering step is vital for vacuole membrane fusion. eLife, 3, e03251.

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Lipid Sourcing for Membrane Experiments

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Myristoleic acid and glycerol monomyristoleate were obtained from Nu-Chek (Elysian, MN), Dioleylphosphatidic acid (DOPA) was obtained from Avanti Polar Lipids (Alabaster, AL), and rhodamine DHPE (lissamine rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine) and NBD DHPE (NBD 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine) were from Life Technologies (Woburn, MA).

Engelhart A.E., Adamala K, & Szostak J.W. (2016). A simple physical mechanism enables homeostasis in primitive cells. Nature chemistry, 8(5), 448-453.

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