All experiments requiring fasting conditions were performed in the afternoon, 5–6 h following the removal of food at the beginning of the light-cycle for reasons outlined elsewhere70 (link). To ensure fasting conditions, mice were transferred to clean cages containing ISO cotton padding and clean cardboard enrichment tubes. Non-terminal blood was collected via tail snip. Glucose tolerance tests (GTT) were performed following the administration of a filtered dextrose (1 g/kg body mass) solution via IP injection71 (link). Insulin tolerance tests (ITT) were performed following the administration of a filtered insulin (0.75 mU/g body mass; Novolin-R, Novo Nordisk, Bagsvaerd, DK) solution via IP injection72 (link). Blood glucose was measured immediately pre-injection (time 0) and at 15, 30, 45, 60, 90, and 120 min post-injection during the GTT and ITT. The area under curve (AUC) for each animal during both the GTT and ITT were also calculated and presented as the average for each group. Blood glucose levels were determined using Accu-Chek Aviva Plus glucometers (Roche, Basel, CH). Fasting insulin levels from blood collected at baseline and during the terminal harvest were evaluated using a Mouse Ultrasensitive Insulin ELISA from Alpco (Salem, NH, USA).
Accu chek aviva plus
Manufactured by Roche
Sourced in Switzerland, United States
The Accu-Chek Aviva Plus is a blood glucose monitoring system designed for people with diabetes. It is a compact, handheld device used to measure blood glucose levels quickly and conveniently.
Automatically generated - may contain errors
Lab products found in correlation
Mouse ultrasensitive insulin elisa, by ALPCO (2 mentions)
Accu chek, by Roche (2 mentions)
Insulin elisa, by Crystal Chem (2 mentions)
Probumin, by Merck Group (2 mentions)
D glucose, by Merck Group (2 mentions)
Spectrophotometry assay, by Sekisui (2 mentions)
Acetaminophen, by Sekisui (2 mentions)
Novolin r, by Novo Nordisk (1 mentions)
Prism v6.0c, by GraphPad (1 mentions)
Humalog, by Eli Lilly (1 mentions)
High fat diet (hfd), by Research Diets (1 mentions)
Bks cg m leprdb j, by Jackson ImmunoResearch (1 mentions)
Elisa colorimetric insulin assay kit, by Crystal Chem (1 mentions)
Labassay triglyceride kit, by Fujifilm (1 mentions)
Nefa hr 2 assay, by Fujifilm (1 mentions)
Ultra sensitive mouse insulin elisa, by Crystal Chem (1 mentions)
Cobas 8000 modular analyzer, by Roche (1 mentions)
Free fatty acid quantification assay kit, by Abcam (1 mentions)
Acetaminophen, by Merck Group (1 mentions)
Ensure plus, by Merck Group (1 mentions)
23 protocols using accu chek aviva plus
1
Fasting Glucose and Insulin Testing in Mice
2
Fasted Mouse Glucose Measurement
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We measured blood glucose using a glucose meter (Roche, Accu-Chek Aviva Plus) in mice that were fasted for 16 hours.
3
Glucose and Insulin Tolerance Tests
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Following 23 weeks of the diet treatments, oral glucose tolerance test (OGTT) was performed. Animals were fasted overnight and given a bolus dose of glucose (2 g/kg body weight) by gavage in the morning. Glucose levels were measured prior to the glucose gavage (0 min) and at 15, 30, 45, 60, 90, and 120 min after the gavage. Glucose concentrations were determined using an Accu-Chek Aviva Plus glucometer and test strips (Roche) with blood obtained through tail prick. Insulin tolerance test (ITT) was performed following 26 weeks of the diet treatments. Animals were fasted overnight and given a dose of insulin (2 mU/g body weight; Humalog, Eli Lilly) by i.p. injection. Glucose levels were measured prior to the insulin injection (0 min) and at 15, 30, 45, 60, 90, and 120 min after the injection. For OGTT, the glucose handling over time was analyzed by calculating the incremental area-under-the-curve (AUC) [31 (link)] using GraphPad Prism v6.0c (GraphPad Software). For ITT, the insulin sensitivity over time was analyzed by calculating the inverse area-under-the-curve (invAUC) below baseline glucose. For calculation of invAUC, the glucose measurements from each animal were subtracted from the baseline glucose value to create an inversed line graph. Then the AUC above zero was calculated using GraphPad Prism v6.0c.
4
Glucose and Insulin Tolerance in Mice
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Intraperitoneal glucose tolerance test (ipGTT) was performed on mice after a 6-h fast and intraperitoneal insulin tolerance test (ITT) was performed after a 3-h fast. Blood samples were drawn at different times following glucose (1mg/kg, i.p) and insulin injection (i.p). Insulin dose is as follows, LFD males: 0.75 U/kg; LFD females: 0.4 U/kg; HFD males: 1 U/kg; HFD females: 0.9 U/kg. Blood glucose concentrations were measured using a glucometer (Accu-Chek Aviva Plus, Roche Diagnostics).
5
High-Fat Diet Induced Obesity in Mice
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Mice either remained on chow diet or were given ad libitum access to a HFD with 60% kcal from fat (Research Diets, New Brunswick, NJ) for 4–8 wk. A dose response of BSA was completed with i.p. injections twice per week at 0, 0.001, 0.01, and 0.1%. Body composition was measured to obtain lean mass and fat mass by nuclear magnetic resonance using a Bruker Minispec instrument in Vanderbilt’s Mouse Metabolic Phenotyping Center during week 4 or 8. Mice were fasted for 6 h during the light cycle for glucose tolerance testing. Fasting blood glucose levels were read using an Accu-Chek Aviva Plus glucometer (Roche) via the tail vein. A 20% glucose solution was administered i.p. at 2 g/kg lean mass, followed by blood glucose readings at 15, 30, 45, 60, 90, and 120 min after glucose injection.
6
Diabetic Wound Healing with Stem Cells
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All animal procedures were performed on genetically diabetic male mice (BKS.Cg-m +/+Leprdb/J; 10 weeks old, Jackson Laboratories, Bar Harbor, ME) in accordance with protocols approved by the Rutgers University institutional animal care and use committee (IACUC) as previously described.1 (link) Briefly, 24 mice were divided into 4 groups (n = 6 per group): (1) phosphate buffered saline (PBS, 50 µL); or hydrogels containing (2) ISCs; (3) MSCs; or both (4) ISC:MSC. Mice were further divided into mice sacrificed on day 14 and on day 28, n = 12 per time point. Thus, for days 1–14, each group had 6 animals and after sacrifice on day 14, each group had 3 animals. The four treatments were applied to full thickness 1 cm × 1 cm excise wounds on the dorsa of the mice. Hydrogels were then covered with Tegaderm™ and photographed at postoperative days 3, 7, 14, 18, 21, and 28 for gross appearance. Wound area was measured using the variance setting of the NIH ImageJ MRI Wound Healing Tool. Wound closure was calculated as percent area of original wound. Mice were weighed on the day of surgery, then weekly until sacrifice. At sacrifice blood was drawn from the tail vein and blood glucose levels were measured with a blood glucose monitoring kit (ACCU-CHEK Aviva Plus; Roche, Basel, Switzerland). Animals were sacrificed by CO2 inhalation and wound tissue was collected for histology.
7
Intravenous Glucose Tolerance Test in ob/ob Mice
8
Intravenous Glucose Tolerance Test in ob/ob Mice
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Following the basal glucose turnover study, the same cohort of ob/ob (B6) mice was subjected to an FSIGT. Blood sampling was performed via an arterial catheter in unrestrained, conscious animals. A continuous infusion of saline-washed erythrocytes was commenced at t = 0 min to prevent a >5% fall in hematocrit. Baseline fasting blood samples were drawn at −10 and 0 min. Based on a published protocol22 (link), a bolus of 50% dextrose (0.75 g/kg body weight) was injected iv over a period of 15s at t = 0 min. Blood (20 μl) was sampled both for measurement of glucose using a hand-held glucometer (Accu-Chek Aviva Plus, Roche; Indianapolis, IN ) and for subsequent assay of plasma insulin and lactate levels at time points 1, 2, 4, 8, 12, 16, 20, 30 and 60 min after the glucose injection. Additional samples were obtained for blood glucose measurement at 3, 5, 6, 10, 14, 18, 25, 40 and 50 min using a hand-held glucometer.
9
Glucose Tolerance and Gastric Emptying
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During glucose tolerance tests, all mice were fasted for 4–5 hr prior to oral administration of dextrose (2 g/kg body weight). Tail vein blood glucose levels were measured using Accu-Chek glucometers (Accu-Chek Aviva Plus, Roche Diagnostics) or Biosen C-line glucose analyzer (EKF diagnostic) at 0, 15, 30, 45, 60, 90, and 120 min post glucose administration. Gastric emptying rate was assessed by an oral gavage of glucose (2 g/kg) with acetaminophen (100 mg/kg) in 4–5 hr fasted mice. Blood was collected from the tail vein at baseline and 10 min after gavage in EDTA-coated microtubes. Plasma acetaminophen levels were used to assess the rate of gastric emptying and were measured using spectrophotometry assay (Sekisui Diagnostics). Plasma insulin levels were determined using ELISA colorimetric insulin assay kit (Crystal Chem).
10
Glucose Tolerance in Surgical Weight Loss
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