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Spinning disc scan head

Manufactured by Yokogawa
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The Spinning Disc Scan Head is a device used in various scientific and industrial applications. It is designed to rapidly scan a sample or surface using a spinning disc mechanism. The core function of this equipment is to provide a high-speed, flexible scanning solution for imaging, analysis, or other specialized tasks. No further details or interpretations are provided.

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Lab products found in correlation

Ixon emccd camera, by Oxford Instruments (4 mentions) Metamorph, by Molecular Devices (4 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions) Ix71 microscope, by Yokogawa (4 mentions) Aotf controlled laser launch, by Oxford Instruments (3 mentions) 35 mm glass bottom dishes, by MatTek (2 mentions) Frappa system, by Oxford Instruments (1 mentions) Mef 2 nucleofector kit, by Lonza (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Spinning disc head (12 citations) CSU-X1 spinning-disc confocal scan head (2 citations)

9 protocols using spinning disc scan head

1

Live Imaging of Hippocampal Neurons

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Live cell imaging of dissociated hippocampal neurons was carried out at 32°C on an Olympus IX71 equipped with a spinning disc scan head (Yokogawa) with a 60× NA1.4 objective. Excitation illumination was delivered from an AOTF controlled laser launch (Andor) and images were collected on a 1024×1024 pixel Andor iXon EM-CCD camera. Data acquisition and analysis were performed with Metamorph (Molecular Devices) and ImageJ (National Institutes of Health). Some images were smoothed (averaging over 3 × 3 pixels) using ImageJ for display, but never before quantification. We simultaneously activated iLID and imaged GFP with a fiber coupled 488 nm laser through the microscope objective (typical photoexcitation conditions were 25% laser power from a 50 mW 488 nm laser, 25–50 ms exposure time).
Zimmerman S.P., Hallett R.A., Bourke A.M., Bear J.E., Kennedy M.J, & Kuhlman B. (2016). Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Biochemistry, 55(37), 5264-5271.
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2

Live Cell Imaging of Transfected HEK293 Cells

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HEK293 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum. Cells were plated on 35-mm glass bottom imaging dishes and transfected using Lipofectamine 2000 (Life Technologies) according to the manufacturer's protocol. Cells were incubated in dark (wrapped with foil) and manipulations were carried out under dim light or using a red safelight. Sixteen to twenty-four hours after transfection, cells were moved to Hepes-buffered Saline (HBS) with 1 mM CaCl2 for imaging. Live cell imaging was performed at 33.5 °C on an Olympus IX71 microscope equipped with a spinning disc scan head (Yokogawa Corporation of America) with a × 60/NA 1.4 objective. Excitation illumination was delivered from an AOTF-controlled laser launch (Andor Technology) and images were collected on a 1,024 × 1,024 pixel EM-CCD camera (iXon; Andor Technology).
Taslimi A., Zoltowski B., Miranda J.G., Pathak G., Hughes R.M, & Tucker C.L. (2016). Optimized second generation CRY2/CIB dimerizers and photoactivatable Cre recombinase. Nature chemical biology, 12(6), 425-430.
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3

Live Cell Imaging of Transfected HEK293 Cells

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HEK293 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum. Cells were plated on 35-mm glass bottom imaging dishes and transfected using Lipofectamine 2000 (Life Technologies) according to the manufacturer's protocol. Cells were incubated in dark (wrapped with foil) and manipulations were carried out under dim light or using a red safelight. Sixteen to twenty-four hours after transfection, cells were moved to Hepes-buffered Saline (HBS) with 1 mM CaCl2 for imaging. Live cell imaging was performed at 33.5 °C on an Olympus IX71 microscope equipped with a spinning disc scan head (Yokogawa Corporation of America) with a × 60/NA 1.4 objective. Excitation illumination was delivered from an AOTF-controlled laser launch (Andor Technology) and images were collected on a 1,024 × 1,024 pixel EM-CCD camera (iXon; Andor Technology).
Taslimi A., Zoltowski B., Miranda J.G., Pathak G., Hughes R.M, & Tucker C.L. (2016). Optimized second generation CRY2/CIB dimerizers and photoactivatable Cre recombinase. Nature chemical biology, 12(6), 425-430.
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4

Fluorescence Microscopy of Transfected Cells

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HEK293 or COS-7 cells were cultured in DMEM with 10% FBS, then seeded onto 18-mm coverslips or 35-mm glass bottom dishes (Mattek) in 12-well plates and transfected using Lipofectamine 2000 (Life Technologies) according to the manufacturer's protocol. Cells were incubated in dark (wrapped with foil) and manipulations were carried out under dim light or using a red safelight. 12-24 hours after transfection, cells were moved to HBS with 1 mM CaCl2 for imaging studies. Live cell imaging was performed at 33.5° C on an Olympus IX71 microscope equipped with a spinning disc scan head (Yokogawa Corporation of America) with a 60x/NA 1.4 objective. Excitation illumination was delivered from an AOTF controlled laser launch (Andor Technology) and images collected on a 1024 × 1024 pixel EM-CCD camera (iXon; Andor Technology). For photoactivation studies (Fig. 5c), laser illumination (488 nm) was delivered using galvometric laser-scanning mirrors (FRAPPA; Andor Technology) at 1-2% of maximum power with a 100 μsec dwell time. Two-photon excitation was performed on a Zeiss LSM510 microscope containing a Chamelon Ultra II laser (Coherent) tuned to 850 nm and 60 % power with a 50 μsec pixel dwell time. For light-sensitive experiments, cells were protected from ambient light sources by focusing in the presence of filtered light (572/28 bandpass filter, Chroma).
Taslimi A., Vrana J.D., Chen D., Borinskaya S., Mayer B.J., Kennedy M.J, & Tucker C.L. (2014). An optimized optogenetic clustering tool for probing protein interaction and function. Nature communications, 5, 4925.
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5

Fluorescence Microscopy of Transfected Cells

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HEK293 or COS-7 cells were cultured in DMEM with 10% FBS, then seeded onto 18-mm coverslips or 35-mm glass bottom dishes (Mattek) in 12-well plates and transfected using Lipofectamine 2000 (Life Technologies) according to the manufacturer's protocol. Cells were incubated in dark (wrapped with foil) and manipulations were carried out under dim light or using a red safelight. 12-24 hours after transfection, cells were moved to HBS with 1 mM CaCl2 for imaging studies. Live cell imaging was performed at 33.5° C on an Olympus IX71 microscope equipped with a spinning disc scan head (Yokogawa Corporation of America) with a 60x/NA 1.4 objective. Excitation illumination was delivered from an AOTF controlled laser launch (Andor Technology) and images collected on a 1024 × 1024 pixel EM-CCD camera (iXon; Andor Technology). For photoactivation studies (Fig. 5c), laser illumination (488 nm) was delivered using galvometric laser-scanning mirrors (FRAPPA; Andor Technology) at 1-2% of maximum power with a 100 μsec dwell time. Two-photon excitation was performed on a Zeiss LSM510 microscope containing a Chamelon Ultra II laser (Coherent) tuned to 850 nm and 60 % power with a 50 μsec pixel dwell time. For light-sensitive experiments, cells were protected from ambient light sources by focusing in the presence of filtered light (572/28 bandpass filter, Chroma).
Taslimi A., Vrana J.D., Chen D., Borinskaya S., Mayer B.J., Kennedy M.J, & Tucker C.L. (2014). An optimized optogenetic clustering tool for probing protein interaction and function. Nature communications, 5, 4925.
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6

Imaging Dissociated Hippocampal Neurons

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Live cell imaging of dissociated hippocampal neurons was carried out
at 34°C on an Olympus IX71 equipped with a spinning disc scan head
(Yokogawa) with a 60x NA1.4 objective. Excitation illumination was delivered
from an AOTF controlled laser launch (Andor) and images were collected on a
1024×1024 pixel Andor iXon EM-CCD camera. Data acquisition and
analysis were performed with Metamorph (Molecular Devices), Andor IQ and
ImageJ software. All quantification was performed on raw images, but some
images were expanded, using the smooth function in ImageJ for display
only.
Sinnen B.L., Bowen A.B., Forte J.S., Hiester B.G., Crosby K.C., Gibson E.S., Dell’Acqua M.L, & Kennedy M.J. (2017). Optogenetic control of synaptic composition and function. Neuron, 93(3), 646-660.e5.
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7

Live Imaging of Cortical Neurons

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Live-cortical neurons were imaged in ACSF at 34 ˚C on an Olympus IX71 equipped with a spinning disc scan head (Yokogawa) with a 60x NA1.4 objective. Excitation illumination was delivered from an AOTF controlled laser launch (Andor) and images were collected on a 1024 × 1024 pixel Andor iXon EM-CCD camera. Data acquisition was performed with Metamorph (Molecular Devices) or Andor IQ software. For some experiments a spinning disk Marianas live-cell imaging system (3i) running Slidebook software (3i) was used. Photoconversion of mEOS3.2 expressing cells was carried out using targeted illumination (FRAPPA system, Andor) with the 405 nm laser (1 s dwell time 5% laser power). For most experiments, a 7 µm Z-stack (0.5 µm step-size) was acquired at each time point.
Bowen A.B., Bourke A.M., Hiester B.G., Hanus C, & Kennedy M.J. (2017). Golgi-independent secretory trafficking through recycling endosomes in neuronal dendrites and spines. eLife, 6, e27362.
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8

Live-Cell Imaging of Dissociated Neurons

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Live cell imaging of dissociated neurons was carried out at 34°C on an Olympus IX71 equipped with a spinning
disc scan head (Yokogawa). Excitation illumination was delivered from an acousto-optic tunable filter (AOTF) controlled laser
launch (Andor). Images were acquired using a 60× Plan Apochromat 1.4 NA objective, and collected on a 1024×1024
pixel Andor iXon EM-CCD camera. Data acquisition and analysis were performed with Metamorph (Molecular Devices) and ImageJ
software.
Liu Q., Sinnen B.L., Boxer E.E., Schneider M.W., Grybko M.J., Buchta W.C., Gibson E.S., Wysoczynski C.L., Ford C.P., Gottschalk A., Aoto J., Tucker C.L, & Kennedy M.J. (2019). A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Neuron, 101(5), 863-875.e6.
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9

Peroxisomal Localization Assay with EGFP-SKL

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For peroxisomal localization studies, a ‘LQSKL’ PTS1 signal sequence from acyl-CoA oxidase was appended to the C-terminus of an EGFP reporter construct (in pCDNA3.1) using PCR. MEFs from wild-type or homozygous mutant Pex10CY embryos were plated on 18-mm glass coverslips in DMEM/F12 (Gibco) with 10% FBS, and transiently transfected with the EGFP-SKL reporter using MEF 2 Nucleofector Kit (Lonza) according to the manufacturer’s protocol. 24 hours after transfection, cells were fixed using 4% paraformaldehyde and imaged on an Olympus IX71 microscope equipped with a spinning disc scan head (Yokogawa Corporation of America) with a 60×/NA 1.4 objective. 488-nm excitation illumination was delivered from an AOTF controlled laser launch (Andor Technology) and images collected on a 1024 × 1024 pixel EM-CCD camera (iXon; Andor Technology).
Hanson M.G., Fregoso V., Vrana J.D., Tucker C.L, & Niswander L.A. (2014). Peripheral nervous system defects in a mouse model for peroxisomal biogenesis disorders. Developmental biology, 395(1), 84-95.
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