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Anti cd5 fitc

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Anti-CD5-FITC is a fluorescent-labeled monoclonal antibody that binds to the CD5 antigen, which is expressed on the surface of T cells and a subset of B cells. This product is intended for use in flow cytometry applications to identify and quantify CD5-positive cells.

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Lab products found in correlation

Anti cd19 apc, by BD (3 mentions) Facscalibur cell analyzer, by BD (2 mentions) Anti cd14 pecy7, by Thermo Fisher Scientific (2 mentions) Anti cd38 fitc, by BD (2 mentions) Anti cd38 apc, by BD (2 mentions) Anti cd3 pe, by BD (2 mentions) Anti cd4 pe texas red, by Thermo Fisher Scientific (1 mentions) Anti cd27 qdot 605, by Thermo Fisher Scientific (1 mentions) Anti bcl 2, by Cell Signaling Technology (1 mentions) Anti apo2.7 pe, by BD (1 mentions) Anti mcl 1 s19, by Santa Cruz Biotechnology (1 mentions) Anti actin, by Merck Group (1 mentions) Ibrutinib pci 32765, by Selleck Chemicals (1 mentions) Anti phosho iκbα, by Cell Signaling Technology (1 mentions) Anti iκbα, by Cell Signaling Technology (1 mentions) Anti bcl xl, by BD (1 mentions) Anti cd3 ecd, by Beckman Coulter (1 mentions) Cd71 fitc, by BD (1 mentions) Anti cd20 ecd, by Beckman Coulter (1 mentions) Anti cd45 pc7, by Beckman Coulter (1 mentions)

Spelling variants of this product

Anti-CD5 (10 citations) CD5-FITC (4 citations)

7 protocols using anti cd5 fitc

1

Tetramer Staining of Antigen-Specific T Cells

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Frozen peripheral blood leukocytes (PBL) were thawed and washed in HSA (0.4 g/L) CO2 independent (Invitrogen) medium before incubation for 1 h with DNAase (10 µg/mL) in the same medium at RT. The cells were then stained with BMFL1 (EBV) (GLCTLVAML) APC-conjugated HLA-A2 tetramers and one of the following PE-conjugated HLA-A2 tetramers all at 20 nM: Melan-A (ELAGIGILTV); MAGE-A3 (KVAELVHFL); MAGE-A1 (KVLEYVIKV), NY-ESO-1 (SLLMWITQV). All tetramers were kindly provided by D. Coleau from LICR, Brussels. After a 30 min incubation at RT, the cells were incubated with anti-CD45RO-Alexa-700 (Becton-Dickinson), anti-CD8β-PE-Cy5.5 (Beckman-Coulter), anti-CD5-FITC (BD), anti-CD4+-PE-Texas-Red, and anti-CD27-Qdot-605 (Invitrogen) for 30 min. After further washes, the cells were acquired on a Canto-B (Becton Dickinson) and analyzed using FlowJo software (Tree-star).
Besse B., Charrier M., Lapierre V., Dansin E., Lantz O., Planchard D., Le Chevalier T., Livartoski A., Barlesi F., Laplanche A., Ploix S., Vimond N., Peguillet I., Théry C., Lacroix L., Zoernig I., Dhodapkar K., Dhodapkar M., Viaud S., Soria J.C., Reiners K.S., Pogge von Strandmann E., Vély F., Rusakiewicz S., Eggermont A., Pitt J.M., Zitvogel L, & Chaput N. (2015). Dendritic cell-derived exosomes as maintenance immunotherapy after first line chemotherapy in NSCLC. Oncoimmunology, 5(4), e1071008.
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2

Flow Cytometry and Immunoblotting Analysis

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The following antibodies were used for flow cytometry analysis: anti-Apo2.7-PE, anti-CD5-FITC, anti-CD19-APC and control IgG1-FITC mAbs were purchased from BD Biosciences (Le Pont de Claix, France). Analysis of protein expression was conducted by immunoblotting using the following primary antibodies: anti-Bcl-2, anti-IκBα and anti-phosho-IκBα (Cell Signaling, Saint Quentin en Yvelines, France), Anti-Mcl-1 (S19) (Santa Cruz Biotechnology, Santa Cruz, CA), anti-Bcl-xL (BD Biosciences, Le Pont de Claix, France), Anti-NF-κB p52 Antibody and anti-actin (Merck Millipore, Lyon, France). ABT-199 was kindly provided by Abbvie Laboratories (North Chicago, IL, USA) and the selective BTK inhibitor ibrutinib (PCI-32765) was obtained from Selleck Chemicals (Souffelweyersheim, France).
Chiron D., Dousset C., Brosseau C., Touzeau C., Maïga S., Moreau P., Pellat-Deceunynck C., Le Gouill S, & Amiot M. (2015). Biological rational for sequential targeting of Bruton tyrosine kinase and Bcl-2 to overcome CD40-induced ABT-199 resistance in mantle cell lymphoma. Oncotarget, 6(11), 8750-8759.
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3

Multiparameter Flow Cytometry Analysis

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Cells were washed with ice-cold PBS once, incubated with appropriate
fluorochrome-conjugated antibodies for 30 min at 4°C and
washed twice with ice-cold PBS containing 0.5% FCS. The following antibodies
were used: anti-CD3-PE (mouse, BD Biosciences, 555333), anti-CD5-FITC (mouse, BD
Biosciences, 555352), anti-CD14-PECy7 (mouse, ebioscience, 25–0149-42),
anti-CD19-APC (mouse, BD Biosciences, 555415), anti-CD27-PE (mouse BD
Biosciences, 555441), anti-CD38-APC (mouse, BD Biosciences, 555462),
anti-CD38-FITC (mouse, BD Biosciences, 555459). Surface protein expression was
detected by a BD FACSCalibur cell analyzer (BD Biosciences) and data were
analyzed using the FlowJo software.
Lee S.H., Singh I., Tisdale S., Abdel-Wahab O., Leslie C.S, & Mayr C. (2018). Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia. Nature, 561(7721), 127-131.
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4

Multiparametric Flow Cytometry of BM

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The composition of cells in BM was tested by flow cytometry, like CD3+ T lymphocytes, CD4+ T cells, CD8+ T cells, Treg cells, and B cells. Anti‐CD45 (PC7, clone: J33, Beckman Coulter; PE‐cy7, clone: HI30, BD bioscience), anti‐CD3 (ECD, clone: UCHT1, Beckman Coulter), anti‐CD4 (PE, clone: SK3, BD bioscience), CD71 (FITC, clone: M‐A712, BD bioscience), anti‐CD8 (FITC, clone: B9.11, Beckman Coulter), anti‐CD25 (Pacific Blue, clone: B1.49.9, Beckman Coulter), anti‐CD127 (PE‐cy5, clone: A019D5, Biolegend), anti‐CD19(PE‐cy5, clone: J3‐119, Beckman Coulter), anti‐CD20 (ECD, clone: B9E9, Beckman Coulter), anti‐CD5 (FITC, clone: L17F12, BD bioscience), anti‐CD10 (PE clone: ALB1, Beckman Coulter) antibodies were purchased. Flow data were acquired on Beckman Coultor Navios (Beckman Coulter, Atlanta, GA) and analyzed using FCS express version 3 software (De Novo Software, Glendale, CA).
Xia L., Wang Y., Li T., Hu X., Chen Q., Liu L., Jiang B., Li C., Wang H., Wang S., Yang G, & Bao Y. (2019). The clinical study on treatment of CD19‐directed chimeric antigen receptor‐modified T cells in a case of refractory Richter syndrome. Cancer Medicine, 8(6), 2930-2941.
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5

Antibody-mediated Cytotoxicity Assay

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Target cells were incubated with Peripheral Blood Mononuclear Cells (PBMCs) at effector to target cell ratios (E:T) 25:1 with rituximab (50 µg/mL), ahuUMG1 (25–100 µg/mL) antibodies or medium alone, o/n at 37°C in RPMI/10% fetal calf serum. Residual T lymphocytes CD8+ (CD45+, CD3+ and CD8+), T lymphocytes CD4+ (CD45+, CD3+ and CD4+), B lymphocytes (CD45+, CD3− and CD19+) and T-ALL cells (CD45dim, CD3 dim/− and CD5+) were analyzed by flow cytometry using a BD FACS CANTO II. The proportion of cells remaining was expressed as a percentage of control cultures incubated without the antibody. The following monoclonal antibodies were used for antigen detection: anti-CD3 PerCP Cy5.5 (Biolegend), anti-CD4 PE (Biolegend), anti-CD8 APC-H7 (Biolegend), anti-CD45 PO (Thermo Fisher Scientific), anti-CD19 PC7 (Beckman Coulter) and anti-CD5 FITC (BD).
Caracciolo D., Riillo C., Ballerini A., Gaipa G., Lhermitte L., Rossi M., Botta C., Duroyon E., Grillone K., Gallo Cantafio M.E., Buracchi C., Alampi G., Gulino A., Belmonte B., Conforti F., Golino G., Juli G., Altomare E., Polerà N., Scionti F., Arbitrio M., Iannone M., Martino M., Correale P., Talarico G., Ghelli Luserna di Rorà A., Ferrari A., Concolino D., Sestito S., Pensabene L., Giordano A., Hildinger M., Di Martino M.T., Martinelli G., Tripodo C., Asnafi V., Biondi A., Tagliaferri P, & Tassone P. (2021). Therapeutic afucosylated monoclonal antibody and bispecific T-cell engagers for T-cell acute lymphoblastic leukemia. Journal for Immunotherapy of Cancer, 9(2), e002026.
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6

Multiparameter Flow Cytometry Analysis

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Cells were washed with ice-cold PBS once, incubated with appropriate
fluorochrome-conjugated antibodies for 30 min at 4°C and
washed twice with ice-cold PBS containing 0.5% FCS. The following antibodies
were used: anti-CD3-PE (mouse, BD Biosciences, 555333), anti-CD5-FITC (mouse, BD
Biosciences, 555352), anti-CD14-PECy7 (mouse, ebioscience, 25–0149-42),
anti-CD19-APC (mouse, BD Biosciences, 555415), anti-CD27-PE (mouse BD
Biosciences, 555441), anti-CD38-APC (mouse, BD Biosciences, 555462),
anti-CD38-FITC (mouse, BD Biosciences, 555459). Surface protein expression was
detected by a BD FACSCalibur cell analyzer (BD Biosciences) and data were
analyzed using the FlowJo software.
Lee S.H., Singh I., Tisdale S., Abdel-Wahab O., Leslie C.S, & Mayr C. (2018). Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia. Nature, 561(7721), 127-131.
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7

Isolation of Murine Peritoneal B-1a and CLL Cells

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Normal peritoneal cells were obtained from 2- to 3-month-old C57BL/6 mice by peritoneal lavage with Hank's buffered salt solution. Anti-B220/CD45RB (APC), anti-CD11b (PE) and anti-CD5 (FITC) antibodies (BD Pharmingen, San Jose, CA) were used to identify and sort B-1a B cells from the peritoneum. Wild-type C57BL/6 mice were used for splenic B-2 cells. Eμ-Tcl1 spleens were obtained from our collaborators at Ohio State University. Eμ-Tcl1 cells were stained with anti-CD5 (FITC) and anti-CD19 (PE or PE-CY7) to determine purity. Peritoneal Eμ-Tcl1 cells were obtained via our adoptive transfer model mice. In the adoptive transfer model, single-cell suspension of 107 Eμ-Tcl1 splenocytes were injected into naive C57/BL6 mice via the intravenous route. Mice were bled weekly after 3 weeks from injection to determine the percentage of B-1 cells in peripheral blood. Once the percentage of B-1 cells (CD5+CD19+) was ≥ 90%, mice were euthanized and peritoneal and splenic CLL cells were obtained.
Alhakeem S.S., Sindhava V.J., McKenna M.K., Gachuki B.W., Byrd J.C., Muthusamy N, & Bondada S. (2015). Role of B cell receptor signaling in IL-10 production by normal and malignant B-1 cells. Annals of the New York Academy of Sciences, 1362(1), 239-249.
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