Acetic acid hac

All agents were obtained commercially as follows: Acrylamide (AM, purity 99%,Aladdin Chemical Co., Ltd.), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (I-2959, purity 98%, Sigma-Aldrich), tannic acid (TA, Mr = 1701.2, Aladdin Chemical Co., Ltd.), gelatin (~ 250 g Bloom, Aladdin Chemical Co., Ltd.), BP (purity 99%, Aladdin Chemical Co., Ltd.), CS (DD = 90%, Shanghai Yuanye Biology), silver nitrate (AgNO₃, purity 99%, SCRC), sodium borohydride (NaBH₄, purity 99%, Aladdin Chemical Co., Ltd.), acetic acid (HAc, purity 99%, Merck), acetone (purity 99%, SCRC), and ethanol (EtOH, purity 99%, SCRC). The planar PVC was purchased from a Shanghai commodity store. All chemicals were used without further purification. Deionized water (Milli-Q system) was used throughout the experiments.

Ibrutinib active ingredient was provided by TOBIO pharmaceuticals. Chitosan (medium molecular weight, and degree of deacetylation ≥85%) and sodium tripolyphosphate (NaTPP, technical grade 85%) were supplied from Sigma-Aldrich. Graphitized carbon nanofibers (200–600 nm outside diameter, 5–50 mm length) were supplied by Grafen Chemical Industries Co. Sulphuric acid (H₂SO₄), nitric acid (HNO₃), methanol (MeOH), and acetic acid (HAc) were of analytical grade and obtained from Merck.

ACN and acetic acid (HAc) were obtained from Merck (Darmstadt, Germany). IAA, urea, NH4HCO3, DTT and TFA were obtained from Sigma-Aldrich (MO, USA). Trypsin was of sequence-grade from Roche Diagnostics (Basel, Switzerland). Ultrapure water was produced by a Milli-Q+ system, Millipore Corp. (MA, USA). Electron track etched polycarbonate filter membranes with a diameter of 13 mm and a pore size of 0.22 μm was obtained from Millipore SAS (Molsheim, Alsace, France) and used as a model surfaces for protein adsorption.

Venusil HILIC sorbent and C18 reverse-phase medium were purchased from Agela Technologies (Tianjin, China). Zwitterionic HILIC (Zic-HILIC) was purchased from Fresh Bioscience (Shanghai, China). The C8 extraction disks were purchased from 3M Empore^™ (St. Paul, MN, USA). Sequencing grade trypsin (A) was obtained from Promega (Madison, WI, USA). Sequencing grade trypsin (B) and Lys-C were obtained from Enzyme & Spectrum (Beijing, China). Dithiothreitol (DTT), iodoacetamide (IAA), formic acid (FA), trifluoroacetic acid (TFA), Tris base and urea were purchased from Sigma (St. Louis, MO, USA). Acetonitrile (ACN) and acetic acid (HAc) were purchased from Merck (Darmstadt, Germany). The quantitative Bradford protein assay kit and colorimetric peptide assay kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and reagents of the best available grade were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA).