Cd20 2h7

Manufactured by BD

CD20 (2H7) is a monoclonal antibody that specifically binds to the CD20 antigen expressed on the surface of B cells. It is commonly used in research and diagnostic applications.

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Lab products found in correlation

Facsymphony a5, by BD (2 mentions) Cd19 sj25c1, by BD (2 mentions) Cd8 sk1, by BD (2 mentions) Cd14 61d3, by Thermo Fisher Scientific (2 mentions) Cd3 sk7, by BD (2 mentions) Igd ia6 2, by BD (2 mentions) Iga is11 8e10, by Miltenyi Biotec (2 mentions) Cd16 cb16, by Thermo Fisher Scientific (2 mentions) Cd71 cy1g4, by BioLegend (2 mentions) Cd38 hb7, by BD (2 mentions) Igg g18 145, by BD (2 mentions) Igm mhm 88, by BioLegend (2 mentions) Accuri flow cytometer, by BD (1 mentions) Facsverse, by BD (1 mentions) Fixation permeabilization solution, by Thermo Fisher Scientific (1 mentions) Nkg2a z199, by Beckman Coulter (1 mentions) Live dead yellow, by Thermo Fisher Scientific (1 mentions) Protein labeling kit, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions) Live dead fixable yellow, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD20 PerCP-Cy5.5 (clone 2H7, (4 citations) CD20 APC-H7 (clone 2H7, (3 citations) Anti-CD20 PE-CF594 (clone 2H7; (3 citations) CD20 (clone 2H7), (2 citations) PE-anti-human CD20 (clone 2H7, (2 citations) CD20–PE/Cy5 (2H7) (2 citations) Anti-CD20 BUV737 (2H7, (2 citations) CD20-PerCP-Cy5.5 (2H7), (2 citations) PE-Cy7 anti-CD20 (2H7) (2 citations) Anti-CD20 (2H7, (2 citations)

3 protocols using cd20 2h7

Multiparametric Flow Cytometry Analysis

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PBMCs, either isolated from the peripheral blood or harvested after a 5-day MLC, were analyzed via cell-surface staining using CD3 (SP34), CD4 (L200), CD8 (SK1), CD20 (2H7), CD25 (M-A251) (all BD Pharmingen), CD16 (NKP15, BD Biosciences), and NKG2a (Z199, Beckman Coulter) antibodies. For chimerism analyses, we used an anti–MHC class I HLA mAb (H38, One Lambda, Inc.) that reacts specifically with an MHC class I antigen on donor but not recipient cells. To assess intracellular protein expression of FOXP3, cells were permeabilized using Fixation/Permeabilization solution (eBioscience) and then stained with anti-FOXP3 mAb (PCH101, BD Pharmingen). Cells were analyzed on a FACSverse (BD Biosciences) or Accuri Flow Cytometer (BD Biosciences) using FlowJo software (FLOWJO LLC).

Hotta K., Aoyama A., Oura T., Yamada Y., Tonsho M., Huh K.H., Kawai K., Schoenfeld D., Allan J.S., Madsen J.C., Benichou G., Smith R.N., Colvin R.B., Sachs D.H., Cosimi A.B, & Kawai T. (2016). Induced regulatory T cells in allograft tolerance via transient mixed chimerism. JCI Insight, 1(10), e86419.

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Phenotyping Antigen-Specific B cells

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Freshly isolated peripheral blood mononuclear cells were stained first for viability with Live/dead Yellow (ThermoFisher) and then for markers with the following monoclonal antibodies: IgA (IS11–8E10, Miltenyi), IgD (IA6–2, BD), IgG (G18–145, BD), IgM (MHM-88, Biolegend), CD3 (SK7, BD), CD4 (RPA-T4, BD), CD8 (SK1, BD), CD14 (61D3, eBioscience), CD16 (CB16, eBioscience), CD19 (SJ25C1, BD), CD20 (2H7, BD), CD27 (O323, BioLegend or M-T271, BD), CD38 (HB7, BD), and CD71 (CY1G4, BioLegend. Antigen-specific B cells were detected by staining with RBD conjugated to Alexa Fluor 488 (Protein Labeling Kit, ThermoFisher). RBD was conjugated according to manufacturer’s instructions, with the following changes: protein was labeled at a concentration of 1mg/mL, and incubated for 30 minutes without the addition of bicarbonate. After staining, PBMCs were washed and then fixed for 30 minutes using 2% paraformaldehyde (ThermoFisher). Data were acquired on a BD FACSymphony A5 and analyzed using FlowJo 10.7.1 (BD).

Mantus G., Nyhoff L.E., Kauffman R.C., Edara V.V., Lai L., Floyd K., Shi P.Y., Menachery V.D., Edupuganti S., Scherer E.M., Kay A., McNair N., Anderson E.J., Rouphael N., Ahmed R., Suthar M.S, & Wrammert J. (2021). Evaluation of Cellular and Serological Responses to Acute SARS-CoV-2 Infection Demonstrate the Functional Importance of the Receptor Binding Domain. Journal of immunology (Baltimore, Md. : 1950), 206(11), 2605-2613.

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Phenotyping SARS-CoV-2-specific B Cells

Cited 1 time

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Freshly isolated or thawed PBMCs were stained first for viability with LIVE/DEAD Fixable Yellow (Thermo Fisher Scientific) and then for markers with the following mAbs: IgA (IS11-8E10; Miltenyi Biotec), IgD (IA6-2; BD Biosciences), IgG (G18-145; BD Biosciences), IgM (MHM-88; BioLegend), CD3 (SK7, BD Biosciences), CD4 (RPA-T4, BD Biosciences), CD8 (SK1; BD Biosciences), CD14 (61D3; eBioscience), CD16 (CB16; eBioscience), CD19 (SJ25C1; BD Biosciences), CD20 (2H7; BD Biosciences), CD27 (O323; BioLegend or MT271; BD Biosciences), CD38 (HB7; BD Biosciences), and CD71 (CY1G4; BioLegend). Ag-specific B cells were detected by staining with RBD-conjugated to Alexa Fluor 488 (Alexa Fluor 488 Protein Labeling Kit; Thermo Fisher Scientific). RBD was conjugated as previously described.⁴ (link) After staining, PBMCs were washed and then fixed for 15 min using 2% paraformaldehyde (PFA; Thermo Fisher Scientific). Data were acquired on a BD FACSymphony A5 and analyzed using FlowJo 10.8.0 (BD Biosciences).

Mantus G., Nyhoff L.E., Edara V.V., Zarnitsyna V.I., Ciric C.R., Flowers M.W., Norwood C., Ellis M., Hussaini L., Manning K.E., Stephens K., Anderson E.J., Ahmed R., Suthar M.S, & Wrammert J. (2022). Pre-existing SARS-CoV-2 immunity influences potency, breadth, and durability of the humoral response to SARS-CoV-2 vaccination. Cell Reports Medicine, 3(4), 100603.

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