FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7, catalog no. 553031 and 553032 respectively), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2, catalog no. 554411) these antibodies were acquired from BD Pharmingen (San Diego, CA). Purified anti-mouse CD16/32 (Fc Block ™, catalog no. 553141) was purchased from BD Pharmingen. FITC-conjugated anti-mouse CD4 (catalog no. 11-0042-82), APC-conjugated CD25 (catalog no. 17-0251-82), FITC-conjugated Gr-1 (catalog no. 11-5931-82) and PE-conjugated CD11b (catalog no. 12-0112-82) antibodies were acquired from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 peptide, E7aa49-57 (RAHYNIVTF), was synthesized by Macromolecular Resources (Denver, CO) with ≥80 % purity. PE-conjugated HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility.
Fitc conjugated gr 1
Manufactured by Thermo Fisher Scientific
Sourced in United States
FITC-conjugated Gr-1 is a fluorescently labeled antibody that binds to the Gr-1 antigen expressed on the surface of certain immune cells. This product is commonly used in flow cytometry applications to identify and analyze these cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Apc conjugated cd25, by Thermo Fisher Scientific (2 mentions)
Pe conjugated cd11b, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated anti mouse cd4, by Thermo Fisher Scientific (2 mentions)
Fitc and pe conjugated anti mouse cd8a clone 53, by BD (2 mentions)
Fitc conjugated anti mouse ifn γ clone xmg1, by BD (2 mentions)
Purified anti mouse cd16 32 fc block, by BD (2 mentions)
70 μm cell strainer, by BD (1 mentions)
Gallios flow cytometer, by Beckman Coulter (1 mentions)
Pe conjugated cd11b, by BD (1 mentions)
Apc conjugated ly6c, by BD (1 mentions)
3 protocols using fitc conjugated gr 1
1
Multicolor Flow Cytometry Analysis
2
Bone Marrow Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bone marrow (BM) cells were isolated by flushing long bones with PBS and filtering through a 70-μm cell strainer (BD, Inc. Cat.#352350). After red blood cell lysis, BM cells were analysed using PE-conjugated CD11b (BD, Inc. Cat.#553311), FITC-conjugated Gr-1 (eBioscience, Inc. Cat.#RB6-8C5) and APC-conjugated Ly6C (BD, Inc. Cat.#557661) antibodies with Gallios flow cytometer (Beckman Coulter, Inc. Brea, CA, USA).
3
Multicolor Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7, catalog no. 553031 and 553032 respectively), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2, catalog no. 554411) these antibodies were acquired from BD Pharmingen (San Diego, CA). Purified anti-mouse CD16/32 (Fc Block ™, catalog no. 553141) was purchased from BD Pharmingen. FITC-conjugated anti-mouse CD4 (catalog no. 11-0042-82), APC-conjugated CD25 (catalog no. 17-0251-82), FITC-conjugated Gr-1 (catalog no. 11-5931-82) and PE-conjugated CD11b (catalog no. 12-0112-82) antibodies were acquired from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 peptide, E7aa49-57 (RAHYNIVTF), was synthesized by Macromolecular Resources (Denver, CO) with ≥80 % purity. PE-conjugated HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility.
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