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Ycc 2

Manufactured by Korean Cell Line Bank
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The YCC-2 is a cell culture incubator designed to provide a controlled environment for the growth and maintenance of cell lines. It features temperature, humidity, and gas concentration regulation to support optimal cell culture conditions.

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Lab products found in correlation

Snu 216, by Korean Cell Line Bank (5 mentions) Snu 668, by Korean Cell Line Bank (5 mentions) Mkn28, by Korean Cell Line Bank (4 mentions) Snu 601, by Korean Cell Line Bank (4 mentions) Snu 16, by Korean Cell Line Bank (3 mentions) Snu 5, by Korean Cell Line Bank (3 mentions) Snu 1, by Korean Cell Line Bank (3 mentions) Snu 638, by Korean Cell Line Bank (3 mentions) Snu 719, by Korean Cell Line Bank (3 mentions) Fetal bovine serum (fbs), by Corning (2 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Welgene (1 mentions) Kato 3, by Korean Cell Line Bank (1 mentions) Mkn45, by Korean Cell Line Bank (1 mentions) Celastrol, by Cayman Chemical (1 mentions) Rpmi 1640 medium, by Corning (1 mentions)

5 protocols using ycc 2

1

Culturing Human Gastric Cancer Cell Lines

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Six human gastric cancer cell lines (AGS, MKN-28, YCC-2, SNU-216, SNU-601, and SNU-668) were obtained from the Korea Cell Line Bank. All cells were cultured in RPMI-1640 medium (Welgene, Korea) containing 5% fetal bovine serum (Corning Costar, USA) and 1% antibiotic-antimycotic (Gibco, USA) in a 37°C incubator in an atmosphere of 5% CO2.
Lee H., Kim W., Kang H.G., Kim W.J., Lee S.C, & Kim S.J. (2019). Geranium thunbergii extract-induced G1 phase cell cycle arrest and apoptosis in gastric cancer cells. Animal Cells and Systems, 24(1), 26-33.
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2

Culturing Human Gastric Cancer Cell Lines

Cited 1 time
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12 human gastric cancer cell lines (AGS, MKN28, YCC-2, KATOIII, SNU-1, SNU-5, SNU-16, SNU-216, SNU-601, SNU-638, SNU-668, and SNU-719) obtained from Korea Cell Line Bank were cultured in DMEM or RPMI 1640 medium supplemented with 5% fetal bovine serum (FBS) and 1% Antibiotics as described previously [45 (link)]. The KCLB authenticate the phenotypes of these cell lines.
Kim S.J., Wang Y.G., Lee H.W., Gu Kang H., La S.H., Ju Choi I., Irimura T., Ro J.Y., Bresalier R.S, & Chun K.H. (2014). Up-regulation of neogenin-1 increases cell proliferation and motility in gastric cancer. Oncotarget, 5(10), 3386-3398.
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3

Gastric Cancer Cell Line Cultivation

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Twelve human gastric cancer cell lines (AGS, MKN28, YCC-2, KATOIII, SNU-1, SNU-5, SNU-16, SNU-216, SNU-601, SNU-638, SNU-668, and SNU-719) obtained from the Korea Cell Line Bank (KCLB, Seoul, Korea) were cultured in RPMI-1640 medium supplemented with 5% fetal bovine serum (FBS) and 1% antibiotics. The phenotypes of these cell lines have been authenticated by the KCLB.
Kim S.J., Lee H.W., Gu Kang H., La S.H., Choi I.J., Ro J.Y., Bresalier R.S., Song J, & Chun K.H. (2014). Ablation of galectin-3 induces p27KIP1-dependent premature senescence without oncogenic stress. Cell Death and Differentiation, 21(11), 1769-1779.
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4

Celastrol Inhibits Cancer Cell Proliferation

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The inhibition of cell proliferation by Celastrol was measured using an MTT assay. Celastrol was purchased from Cayman Chemical (Michigan, USA) and dissolved in dimethyl sulfoxide (DMSO). The human stomach adenocarcinoma cell line AGS and the gastric carcinoma cell lines MKN1, MKN45, SNU216, SNU-668, and YCC-2 were purchased from the Korea Cell Line Bank (KCLB, Seoul, Korea). The KCLB authenticates the phenotypes of these cell lines on a regular basis. The analysis of cell proliferation using MTT assay and migration using a woundhealing assay was performed as described previously (32) (link).
Lee H.W., Jang K.S., Choi H.J., Jo A., Cheong J.H, & Chun K.H. (2014). Celastrol inhibits gastric cancer growth by induction of apoptosis and autophagy. BMB Reports, 47(12), 697-702.
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5

Gastric Cancer Cell Line Culturing

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Twelve human gastric cancer cell lines (AGS, YCC-2, MKN28, KATO III, SNU-1, SNU-5, SNU-16, SNU-216, SNU-601, SNU-638, SNU-668, and SNU-719) obtained from the Korea Cell Line Bank (KCLB, Korea) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS: Corning Costar, USA) and 1% antibiotic-antimycotic (Gibco, USA). Cell cultures were maintained at 37°C in an atmosphere of 5% CO2. The phenotypes of these cell lines have been authenticated by the KCLB.
Kim H.Y., Cho Y., Kang H., Yim Y.S., Kim S.J., Song J, & Chun K.H. (2016). Targeting the WEE1 kinase as a molecular targeted therapy for gastric cancer. Oncotarget, 7(31), 49902-49916.
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