Synthetic mir 9 mimic

Erlotinib (qE-4007) was purchased from LC Laboratories, and dissolved in DMSO at 20 mmol/L. 5-Azacytidine (A2385) was purchased from Sigma-Aldrich, and dissolved in PBS at 20 mmol/L. Stock solutions were at −20 °C and diluted just before use. Lipofectamine 2000 transfection reagent was purchased from Life Technologies Co. Invitrogen (11668-019). Antibodies FoxO1 (BS3573), actin (AP0064), and GAPDH (AP0063) were purchased from Bioworld Technology Inc. NFκB p105/p50 antibody (1559-1) was purchased from Epitomics, Inc. The synthetic miR-9 mimic, miR-9 inhibitor, and their relative control were purchased from Dharmacon.

The synthetic miR-9 mimic, miR-9 inhibitor, and their relative control were purchased from Dharmacon. Cells seeded in 6-well plate at 5 × 10⁵ cells/well were transfected with synthetic miR-9 and its control using lipofectamine 2000, or infected with lentivirus aforementioned for 24 h. Then cells were reseeded to 96-well plates for a 5-day SRB assay, or 6-well plates for another 24 h for qRT-PCR assay and western blot analysis.