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G1603a agilent ce ms adapter

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The G1603A Agilent CE-MS adapter is a device that connects a capillary electrophoresis (CE) system to a mass spectrometer (MS). It facilitates the transfer of samples from the CE system to the MS for analysis.

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Lab products found in correlation

Agilent g1607a ce esi ms sprayer kit, by Agilent Technologies (7 mentions) Agilent 1100 series binary hplc pump, by Agilent Technologies (6 mentions) Agilent ce system, by Agilent Technologies (5 mentions) Vd 800r lyophilizer, by TAITEC (5 mentions) Agilent g3250aa lc msd tof system, by Agilent Technologies (4 mentions) G3250aa lc msd tof system, by Agilent Technologies (3 mentions) Agilent ce capillary electrophoresis system, by Agilent Technologies (3 mentions) Agilent chemstation software, by Agilent Technologies (1 mentions) Agilent mass hunter software, by Agilent Technologies (1 mentions) G7100 60041 platinum esi needle, by Agilent Technologies (1 mentions) Masshunter software, by Agilent Technologies (1 mentions)

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Agilent G1603A CE-MS adapter kit (24 citations) G1603A Agilent CE-MS adapter- and G1607A Agilent CE-ESI–MS sprayer kit (2 citations)

9 protocols using g1603a agilent ce ms adapter

1

Fecal Metabolome Analysis by CE-TOFMS

Cited 1 time
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Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)-based metabolome analysis of fecal samples was conducted as described previously (Hirayama et al., 2012 (link)) with some modifications. In brief, fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 h. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using the Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol (Wang et al., 2015 (link)). CE-TOFMS experiments were performed using the Agilent CE System, the Agilent G3250AA LC/MSD TOF System, the Agilent 1100 Series Binary HPLC Pump, the G1603A Agilent CE-MS adapter, and the G1607A Agilent CE-ESI-MS Sprayer kit. In-house software (MasterHands) (Sugimoto et al., 2010 (link)) was used for data processing, quantification, and peak annotation.
Tanaka K., Tanigawa N., Song I., Komatsu T., Kuriki Y., Tanaka Y., Fukudo S., Urano Y, & Fukuda S. (2023). A protease activity-based machine-learning approach as a complementary tool for conventional diagnosis of diarrhea-predominant irritable bowel syndrome. Frontiers in Microbiology, 14, 1179534.
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2

Fecal Metabolome Analysis via CE-TOFMS

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Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)–based metabolome analysis was conducted as described previously with some modifications.28 (link) In brief, fecal samples were lyophilized by using a VD-800R lyophilizer (TAITEC, Saitama, Japan) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science, Tokyo, Japan) by vigorous shaking (1500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted by the methanol:chloroform:water extraction protocol. CE-TOFMS experiments were performed using the Agilent CE System, the Agilent G3250AA LC/MSD TOF System, the Agilent 1100 Series Binary HPLC Pump, the G1603A Agilent CE-MS adapter, and the G1607A Agilent CE-ESI-MS SprayerKit (all Agilent Technologies, Santa Clara, CA).
Nagao-Kitamoto H., Shreiner A.B., Gillilland MG I.I.I., Kitamoto S., Ishii C., Hirayama A., Kuffa P., El-Zaatari M., Grasberger H., Seekatz A.M., Higgins P.D., Young V.B., Fukuda S., Kao J.Y, & Kamada N. (2016). Functional Characterization of Inflammatory Bowel Disease–Associated Gut Dysbiosis in Gnotobiotic Mice. Cellular and Molecular Gastroenterology and Hepatology, 2(4), 468-481.
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3

Fecal Metabolome Analysis by CE-TOFMS

Cited 1 time
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Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)–based metabolome analysis was conducted as described previously with some modifications 44 (link). In brief, fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using ShakeMaster (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol. CE-TOFMS experiments were performed using the Agilent CE System, the Agilent G3250AA LC/MSD TOF System, the Agilent 1100 Series Binary HPLC Pump, the G1603A Agilent CE-MS adapter and the G1607A Agilent CE-ESI-MS Sprayer Kit (Agilent Technologies). MasterHands software was used for data processing, quantification and peak annotation 45 (link).
Kitamoto S., Alteri C.J., Rodrigues M., Nagao-Kitamoto H., Sugihara K., Himpsl S.D., Bazzi M., Miyoshi M., Nishioka T., Hayashi A., Morhardt T.L., Kuffa P., Grasberger H., El-Zaatari M., Bishu S., Ishii C., Hirayama A., Eaton K.A., Dogan B., Simpson K.W., Inohara N., Mobley H.L., Kao J.Y., Fukuda S., Barnich N, & Kamada N. (2019). Dietary L-serine confers a competitive fitness advantage to Enterobacteriaceae in the inflamed gut. Nature microbiology, 5(1), 116-125.
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4

Metabolomic Analysis of Intestinal Epithelium

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Intestinal epithelium samples were isolated and disrupted with 3.0-mm Zirconia Beads (Biomedical Science) in methanol by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Cellular metabolites were extracted using the methanol:chloroform:water extraction protocol. Time-of-flight mass spectrometry (CE-TOFMS)–based metabolome experiments were performed using the Agilent CE System, the Agilent G3250AA LC/MSD TOF System, the Agilent 1100 Series Binary HPLC Pump, the G1603A Agilent CE-MS adapter and the G1607A Agilent CE-ESI-MS Sprayer Kit (Agilent Technologies, USA)60 (link). In-house software (MasterHands) was used for data processing, quantification and peak annotation61 (link). Heatmaps were drawn with normalised z-score using R software (ver. 3.5.3).
Nakamura A., Kurihara S., Takahashi D., Ohashi W., Nakamura Y., Kimura S., Onuki M., Kume A., Sasazawa Y., Furusawa Y., Obata Y., Fukuda S., Saiki S., Matsumoto M, & Hase K. (2021). Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage differentiation in the colon. Nature Communications, 12, 2105.
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5

Agilent CE-TOF-MS Instrumentation for Anion Analysis

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All CE-TOF-MS experiments were performed using an Agilent CE capillary electrophoresis system (Agilent Technologies, Waldbronn, Germany), Agilent G1969A and G6220A Accurate-Mass TOF LC-MS system (Agilent Technologies, Palo Alto, CA, USA), Agilent 1100 and 1200 series isocratic high-performance LC pumps, G1603A Agilent CE-MS adapter, and Agilent CE electrospray ionization (ESI)-MS sprayer kit (G1600AX and G7100A). An Agilent G1607-60001 platinum ESI needle was used for anion analysis. The Agilent ChemStation software (ver. A.10.02, B.02.01.SR1, and B.03.02, C.01.07.SE1, Agilent Technologies, Waldbronn, Germany) for CE and the Agilent MassHunter software (ver. B.02.00, Agilent Technologies, Palo Alto, CA, USA) was used for the system control and data acquisition.
Iwamoto H., Okihara M., Akashi I., Kihara Y., Konno O., Kawachi S., Sunamura M, & Sugimoto M. (2022). Metabolomic Profiling of Plasma, Urine, and Saliva of Kidney Transplantation Recipients. International Journal of Molecular Sciences, 23(22), 13938.
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6

Fecal Metabolome Analysis by CE-TOFMS

Cited 1 time
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Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)–based metabolome analysis was conducted as described previously with some modifications 44 (link). In brief, fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using ShakeMaster (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol. CE-TOFMS experiments were performed using the Agilent CE System, the Agilent G3250AA LC/MSD TOF System, the Agilent 1100 Series Binary HPLC Pump, the G1603A Agilent CE-MS adapter and the G1607A Agilent CE-ESI-MS Sprayer Kit (Agilent Technologies). MasterHands software was used for data processing, quantification and peak annotation 45 (link).
Kitamoto S., Alteri C.J., Rodrigues M., Nagao-Kitamoto H., Sugihara K., Himpsl S.D., Bazzi M., Miyoshi M., Nishioka T., Hayashi A., Morhardt T.L., Kuffa P., Grasberger H., El-Zaatari M., Bishu S., Ishii C., Hirayama A., Eaton K.A., Dogan B., Simpson K.W., Inohara N., Mobley H.L., Kao J.Y., Fukuda S., Barnich N, & Kamada N. (2019). Dietary L-serine confers a competitive fitness advantage to Enterobacteriaceae in the inflamed gut. Nature microbiology, 5(1), 116-125.
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7

CE-TOFMS Analysis of Diverse Analytes

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All CE-TOFMS experiments were performed using an Agilent CE capillary electrophoresis system (Agilent Technologies, Waldbronn, Germany), Agilent 1669A Accurate-Mass TOF LC/MS system (Agilent Technologies, Palo Alto, CA, USA), Agilent 1100 and 1200 series isocratic high-performance LC pump, G1603A Agilent CE-MS adapter and G1600AX Agilent CE electrospray ionization (ESI)-MS sprayer kit. For anion analysis, an Agilent G7100-60041 platinum ESI needle was used. Agilent ChemStation software (ver. A.10.02, B.02.01.SR1, and B.03.02) for CE and Agilent MassHunter software (ver. B.02.00 and B.02.02) were used for system control and data acquisition.
Yamano E., Sugimoto M., Hirayama A., Kume S., Yamato M., Jin G., Tajima S., Goda N., Iwai K., Fukuda S., Yamaguti K., Kuratsune H., Soga T., Watanabe Y, & Kataoka Y. (2016). Index markers of chronic fatigue syndrome with dysfunction of TCA and urea cycles. Scientific Reports, 6, 34990.
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8

Capillary Electrophoresis-Mass Spectrometry for Metabolites

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Plasma, urinary, and fecal levels of fecal purine metabolites including allantoin were measured using capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) as described previously [15 (link)]. All CE-TOFMS experiments were performed using an Agilent CE capillary electrophoresis system (Agilent Technologies, Santa Clara, CA, USA), an Agilent G3250AA LC/MSD TOF system (Agilent Technologies), an Agilent 1100 series binary HPLC pump, a, G1603A Agilent CE-MS adapter, and a G1607A Agilent CE-ESI-MS sprayer kit.
Mishima E., Ichijo M., Kawabe T., Kikuchi K., Akiyama Y., Toyohara T., Suzuki T., Suzuki C., Asao A., Ishii N., Fukuda S, & Abe T. (2020). Germ-Free Conditions Modulate Host Purine Metabolism, Exacerbating Adenine-Induced Kidney Damage. Toxins, 12(9), 547.
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9

CE-TOFMS Fecal Metabolome Analysis

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CE-TOFMS-based metabolome analysis was conducted as described previously with some modifications44 (link). In brief, fecal samples were lyophilized by using VD-800R lyophilizer (TAITEC) for 24 h. Freeze-dried feces were disrupted with 3.0 mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 r.p.m. for10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted by the methanol:chloroform:water extraction protocol. CE-TOFMS experiments were performed using the Agilent CE System (Agilent Technologies), the Agilent G3250AA LC/MSD TOF System (Agilent Technologies), the Agilent 1100 Series Binary HPLC Pump, the G1603A Agilent CE-MS adapter (Agilent Technologies), and the G1607A Agilent CE-ESI-MS SprayerKit.
Nagao-Kitamoto H., Shreiner A.B., Gillilland MG I.I.I., Kitamoto S., Ishii C., Hirayama A., Kuffa P., El-Zaatari M., Grasberger H., Seekatz A.M., Higgins P.D., Young V.B., Fukuda S., Kao J.Y, & Kamada N. (2016). Functional Characterization of Inflammatory Bowel Disease–Associated Gut Dysbiosis in Gnotobiotic Mice. Cellular and Molecular Gastroenterology and Hepatology, 2(4), 468-481.
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