Agilent 1260 high performance liquid chromatography

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1260 High Performance Liquid Chromatography (HPLC) is an analytical instrument used for the separation, identification, and quantification of chemical compounds in a liquid sample. It employs high pressure to propel the mobile phase and sample through a stationary phase, enabling efficient separation and detection of the components within the sample.

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Lab products found in correlation

Eclipse plus c18 analytical column, by Agilent Technologies (1 mentions) High performance liquid chromatography (hplc), by Agilent Technologies (1 mentions) 214 polyma differential scanning calorimeter, by Netzsch (1 mentions) Ht7700 transmission electron microscopy, by Hitachi (1 mentions) Agilent 1290 6545, by Agilent Technologies (1 mentions) Sodium hydroxide (naoh), by Sinopharm (1 mentions) Sulfuric acid h2so4, by Sinopharm (1 mentions)

Close spelling variants of this product

Agilent 1260 (504 citations) Agilent 1260 high (7 citations) Agilent 1260 high-performance liquid chromatography system (4 citations) Agilent 1260 Infinity II High Performance Liquid Chromatography (2 citations) Agilent 1260 Infinity High-Performance Liquid Chromatography ( (2 citations)

11 protocols using agilent 1260 high performance liquid chromatography

Quantitative Analysis of COR and CGR

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1.0 g COR and CGR powder was accurately weighed respectively, accurately added 50 mL of methanol, weighed it, heated to re ux for 2 h. The mixture was cooled down to room temperature and weighed it again, methanol were added to make up the lost weight, shook and ltered through lter paper. Took 20 mL of the ltrate and evaporated to dryness, dissolved the residue with methanol, transferred to a 10 mL volumetric ask, then added methanol to the mark to obtain the sample solution. The solution was ltered through a 0.22 µm membrane lter before injection into the HPLC system.
The sample solution of COR and CGR were analyzed by Agilent 1260 High Performance Liquid Chromatography (Agilent Technologies, CA, USA) equipped with a Zorbax Eclipse Plus C18 analytical column (4.6 mm × 150 mm, 5 µm) and a guard column. The temperature was set at 30 ℃, the injection volume was 10 µL, and the detection wavelength was set to 285 nm. A binary elution at a ow rate of 1.0 mL/min was employed using an aqueous phase of 0.1% formic acid as solvent A and acetonitrile as solvent B, the gradient elution procedure was A:B = 21:79, detection time was 18 minutes.

Liu Y., Guo Y., Liu J., Bao X., Zhou J., Jia P., Liu F., Liu J., Wu Z., & Guo L. (2020). Differentiating Curculigo Orchioides Rhizoma and Curculigo glabrescens Rhizoma by a Unique New Compound.

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Spray Drying and Analytical Techniques

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SP‐1500 Laboratory Spray Dryer (Shanghai Shunyi Experimental Equipment Co., Ltd.); Agilent 1260 High Performance Liquid Chromatography (Agilent Technologies, Inc.); Kc‐180w Ultrasonic cleaning instrument (Jining Keyuan Ultrasonic Equipment Co., Ltd.); AB105 electronic analytical balance (Shanghai Precision Instrument Co., Ltd.); KYKY‐EM6200 scanning electron microscope (SEM, Beijing Zhongke Technological Development Co., Ltd.); BT‐9300H Laser Particle Size Analyzer (Dandong Baxter Instrument Co., Ltd.); and ZRS‐8G intelligent dissolution tester (Tianjin Tianda Tianfa Technology Co., Ltd.).

Zhang Z., Li L., Sun D., Wang M., Shi J., Yang D., Wang L, & Zou S. (2020). Preparation and properties of chitosan‐based microspheres by spray drying. Food Science & Nutrition, 8(4), 1933-1941.

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Quantification of Free Amino Acids in Soup

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According to a previously described method [18 (link)], we weighed 1 mL soup, added sulfosalicylic acid (10%) in the ratio of 1:1, stood for a period of time, centrifuged (9600 r/min, 10 min, 4 °C) the sample, took the supernatant through a 0.45 μm filter membrane, and waited for the sample to be analyzed by HPLC (Agilent Technology Co., Ltd., Beijing China).
The free amino acids in the samples were analyzed by Durashell AA kit. Agilent 1260 high performance liquid chromatography (HPLC) was used to analyze 17 kinds of free amino acids in the standard and sample. The 17 kinds of free amino acids were Asp, Glu, Ser, Pro, Gly, Thr, Ala, Val, Met, Ile, Phe, Lys, Leu, Arg, His, Tyr, and Cys-Cys. Determination conditions: the chromatographic column was Durashell AA (4.6 mm × 150 mm, 3 μm); the mobile phase A was (9.50 g sodium tetraborate decahydrate and 9.00 g disodium hydrogen phosphate dodecahydrate were weighed, respectively; 2000 mL ultrapure water was added; pH was adjusted to 8.20 with 36% hydrochloric acid); the mobile phase B was (45% acetonitrile, 45% methanol, and 10% ultrapure water); the gradient elution method was 6–10% B 0–6 min, 10% B 6–8 min, 10–16% B 8–10 min. The flow rate of the mobile phase was 1.60 mL/min; the column temperature was 45 °C; the detection wavelengths were 338 nm and 262 nm, respectively.

Duan W., Liang L., Huang Y., Zhang Y., Sun B, & Li L. (2021). Effect of Ginger on Chemical Composition, Physical and Sensory Characteristics of Chicken Soup. Foods, 10(7), 1456.

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Dissolution Study of YZDT Compound

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The dissolution study was performed by placing the YZDT in 200 mL of ultra-pure water that had been treated by ultrasound for 30 minutes, using the small cup method at 100 rpm and 37 ± 0.5 °C. Dissolution medium (2 mL) was withdrawn at 5, 10, 20, 30 and 40 min through a 0.22 μm microporous membrane^{32 (link)}. The sample was analyzed by LC-MS (Agilent 1260 high performance liquid chromatography, Agilent, USA; Agilent 6460 C Triple Quadrupole LC/MS with ESI ion source, Agilent, USA), each YZDT was paralleled six times and five components were determined.

Li P., Feng B., Jiang H., Han X., Wu Z., Wang Y., Lin J., Zhang Y., Yang M., Han L, & Zhang D. (2018). A Novel Forming Method of Traditional Chinese Medicine Dispersible Tablets to Achieve Rapid Disintegration Based on the Powder Modification Principle. Scientific Reports, 8, 10319.

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Analytical Instrumentation for Multidisciplinary Research

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Agilent 1260 high-performance liquid chromatography (HPLC) was purchased from Agilent (California, USA), HT7700 transmission electron microscopy (TEM) was purchased from Hitachi (Tokyo, Japan), 214 polyma differential scanning calorimeter (DSC) was purchased from Netzsch (Selbe, Germany), Nicolet 380 fourier transform infrared spectroscopy was purchased from Hitachi (Tokyo, Japan), Delsa Nano size analyzer was purchased from Beckman Coulter (Kraemer Boulevard Brea, USA), ZH-YLS-1A animal locomotor actimeter was purchased from Zhenghua Biologic Apparatus facilities company (Anhui, China).

Li B., Han L., Cao B., Yang X., Zhu X., Yang B., Zhao H, & Qiao W. (2019). Use of magnoflorine-phospholipid complex to permeate blood-brain barrier and treat depression in the CUMS animal model. Drug Delivery, 26(1), 566-574.

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Licorice Metabolite Profiling in China

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A total of 21 batches (S1–S21) of licorice samples were collected from different areas in 6 provinces of China: Wuzhong, Ningxia (S1, S2, S3, S4), Longde County, Guyuan City, Ningxia (S5, S6), Lanzhou City, Gansu Province (S7, S8), Wuwei City, Gansu Province (S9), Dingxi City, Gansu Province (S10, S11), Qingyang City, Gansu Province (S12), Baiyin City, Gansu Province (S13), Chifeng City, Inner Mongolia (S14, S15), Erdos City, Inner Mongolia (S16), Luliang City, Shanxi Province (S17), Alar City, Xinjiang Province (S18), Yili, Xinjiang Province (S19, S20), Harbin, and Heilongjiang Province (S21). All batches were authenticated as Glycyrrhiza uralensis Fisch. by the pharmacognosy department of Ningxia Medical University.
All standards were purchased from Shanghai Yuanye biotechnology Co., LTD. HPLC-grade acetonitrile, anhydrous ethanol, acetic acid, and methanol were obtained from the Tianjin Damao chemical reagent factory. Also, the instrument used: UV-2600 visible - ultraviolet spectrophotometer (Shimazu instrument Co., LTD), high-performance liquid chromatography-quadrupole in-flight mass spectrometry (Agilent 1290 + 6545), Agilent 1260 high-performance liquid chromatography (Agilent technology co. LTD), etc.

Xia W., Liu Q., Zhou H., Hua S., Dong L., Han X, & Fu X. (2021). Study on the Spectrum-Effect Relationship of the Traditional Effect of Saponins in Glycyrrhiza uralensis Fisch. International Journal of Analytical Chemistry, 2021, 6617033.

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Comprehensive Analytical Techniques for Research

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The instruments used are Agilent 1260 High Performance Liquid Chromatography (Agilent Technologies, USA), Millipore Simplicity-185 Ultra Water Purifier (Millipore Corporation, USA), JIN92-IIN Ultrasonic Cell Shredder (Ningbo Xinzhi Biotechnology Co., Ltd.), and Nano S90 Laser Particle Size Analyzer (British Malvern).

Luo Y., Yang W., Abuduaini A, & Aisa H.A. (2018). Preparation of Magnetic Nanoliposomes of Sesquiterpene-Rich Fraction from Cichorium glandulosum and Its Tissue Distribution in Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 8549519.

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HPLC Analysis of Phytochemicals

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The Agilent 1260 High performance liquid chromatography (Agilent Technologies, USA), with an Agilent TC-C18(2) chromatographic column (4.6×250 mm, 5 μm) was utilized. The flow rate was 1 mL·min^-1, column temperature was 30 °C, detection wavelength was 280 nm, and injection volume was 5 μL. The mobile phase comprised methanol (A) and –0.1% formic acid in water (B). The gradient elution procedure was: 0–6 min, 10%–30% A; 6–13 min, 30%–33% A; 13–21 min, 33%–37% A; 21–25 min, 37%–40% A; 25–35 min, 40%–45% A; 35–45 min, 45%–48% A; 45–49 min, 48%–52% A; 49–54 min, 52%–60% A; 54–64 min, 60%–95% A; 64–74 min, 95%–10% A; 74–78 min, 10% A.

Guo Y., Cao Q., Guo M., Wang J., Kou R, & Ye L. (2022). Comparative study of the Rheum tanguticum’s chemical contents based on spatial distribution characteristics. PLOS ONE, 17(11), e0278113.

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Wastewater Treatment Optimization Protocol

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Reagents: polymerized aluminum chloride (PAC, 35%, Gongyi Tenglong Water Treatment Material Co., Ltd., Gongyi, China);

{Na}_{2} {FeO}_{4}

(99%, Guangzhou Wuxuan Chemical Co., Ltd., Guangzhou, China); sodium hydroxide (NaOH, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China); sulfuric acid (

H_{2} {SO}_{4}

, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China); methanol (Methanol, 99%, Sigma Aldrich (Shanghai) Trading Co., Ltd., Shanghai, China).
Instruments: Agilent 1260 High-Performance Liquid Chromatography, Agilent Technologies (China) Co., Ltd. (Beijing, China); HH.S11-2-3 Electric Thermostatic Water Bath, Shanghai Yuejin Medical Equipment Factory; PHB-5 Handheld pH Meter, Hangzhou Dewei Instrument Technology Co., Ltd. (Hangzhou, China); DR890 Portable Visible Spectrophotometer, HACH Water Quality Analysis Instrument (Shanghai) Co., Ltd. (Shanghai, China); COD digestion instrument, Taizhou Huachen Instrument Co., Ltd. (Taizhou, China).

Zhang C., Chen X., Chen M., Ding N, & Liu H. (2023). Response Surface Optimization on Ferrate-Assisted Coagulation Pretreatment of SDBS-Containing Strengthened Organic Wastewater. International Journal of Environmental Research and Public Health, 20(6), 5008.

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Acenaphthene Sorption Kinetics on Biochar

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To assess sorption kinetics, 0.005 g of biochar was accurately weighed and placed in a 40 mL brown sample tube, to which 40 mL of an acenaphthene solution (3.5 mg L⁻¹) was added. Each group were conducted in triplicate, and a blank control without biochar were included. The samples were placed in a shaker set at 30 °C and 120 rpm and were taken out after 0, 5, 12, 24, or 48 h. Upon filtering the samples with a 0.22 μm hydrophilic polytetrafluoroethylene (PTFE) syringe filter, the acenaphthene concentration in the filtrate was determined by Agilent 1260 high-performance liquid chromatography (USA).
For the batch equilibrium experiment, based on the kinetics experiment, the acenaphthene concentration in the solution was determined after sorption equilibrium was achieved at each of the Triton X-100 concentrations (0, 50, 200, and 300 mg L⁻¹). Taking the equilibrium concentration of acenaphthene as the abscissa and the sorption capacity of acenaphthene by S500 as the ordinate, the equilibrium sorption capacity of biochar was obtained under different conditions according to eqn (1): where C₀ is the initial concentration of acenaphthene (mg L⁻¹); C_t is the concentration of acenaphthene after equilibrium is achieved (mg L⁻¹); V is the volume of solution (L); m is the mass of biochar (g); and Q_e is the sorption capacity (mg g⁻¹).

Lu L., Li A., Ji X., Yang C, & He S. (2018). Removal of acenaphthene from water by Triton X-100-facilitated biochar-immobilized Pseudomonas aeruginosa. RSC Advances, 8(41), 23426-23432.

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