Quinpirole

Manufactured by Bio-Techne

Sourced in United Kingdom, United States

Quinpirole is a synthetic chemical compound that is commonly used as a laboratory tool in scientific research. It functions as a selective agonist for the D2 and D3 dopamine receptors, which are important in the study of the dopaminergic system and its role in various biological processes and neurological disorders.

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Lab products found in correlation

Sulpiride, by Bio-Techne (13 mentions) Sch23390, by Bio-Techne (7 mentions) Sulpiride, by Merck Group (6 mentions) Sch23390, by Merck Group (6 mentions) Skf81297, by Bio-Techne (6 mentions) Dopamine, by Merck Group (5 mentions) Picrotoxin, by Bio-Techne (5 mentions) Cgp55845, by Bio-Techne (5 mentions) Skf38393, by Bio-Techne (5 mentions) Picrotoxin, by Merck Group (3 mentions) Acetylcholine, by Merck Group (3 mentions) L 745870, by Bio-Techne (3 mentions) D ap5, by Bio-Techne (3 mentions) Dopamine hcl, by Merck Group (3 mentions) Alexa 594, by Thermo Fisher Scientific (2 mentions) Fluo 5f, by Thermo Fisher Scientific (2 mentions) Phenylephrine, by Bio-Techne (2 mentions) Cgs21680, by Bio-Techne (2 mentions) Ascorbic acid, by Merck Group (2 mentions) Bapta, by Merck Group (2 mentions)

Close spelling variants of this product

Quinpirole hydrochloride (23 citations) Quinpirole HCl (4 citations)

42 protocols using quinpirole

Dopaminergic Regulation of Th17 Cell Function

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To evaluate the function of Th17-cells, CD4⁺ T-cells were isolated and activated by anti-CD3/CD28-microbeads as previously described [17 (link)].
To assess the effect of dopamine on the function of Th17-cells, samples of CD4⁺ T-cells were cultured in the presence of dopamine (Sigma, USA) at a concentration of 10^–5 M [9 (link)] for 15 min. whereafter anti-CD3/CD28-microbeads were added to the cultures.
To study the involvement of dopaminergic receptors in dopamine-mediated modulation of cytokine production, some samples of CD4⁺ T-cells were pre-incubated with antagonists of D₁- or D₂-like dopaminergic receptors (SCH23390 and sulpiride respectively) (both from Sigma, USA) at a concentration of 10^–5 M [9 (link)] for 15 min, whereafter dopamine (at 10^–5 M) was added to the cultures and stimulation was proceeded.
To study the direct effect of blockading or activation of the dopaminergic receptor, CD4⁺ T-cells were pre-incubated in the presence of D₁- or D₂-like receptors antagonists (SCH23390 and sulpiride respectively [at 10^–5 M]) or D₂-like receptor agonist (quinpirole [at 10^–7 M] Tocris, Switzerland) and activated by anti-CD3/CD28-microbeads [18 (link)].

Melnikov M., Sviridova A., Rogovskii V., Kudrin V., Murugin V., Boyko A, & Pashenkov M. (2022). The Role of D2-like Dopaminergic Receptor in Dopamine-mediated Modulation of Th17-cells in Multiple Sclerosis. Current Neuropharmacology, 20(8), 1632-1639.

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Optimized Reagent Procurement for Research

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Dopamine hydrochloride (#H-8502), S-(-)3-PPP (#P-103), Terguride (#T-134), angiotensin II (#A9525), and buprenorphine hydrochloride (#B9275) were purchased from Sigma-Aldrich; sulpiride (#0895), quinpirole (#1061), bromocriptine (#0427), olmesartan (#4616), Dynorphin B (#3196), naloxone hydrochloride (#0599), WN552122 (#1038), and rimonabant hydrochloride (#0923) from Tocris; DAMGO (#024-10) from Phoenix Pharmaceuticals, and rapamycin (#tlrl-rap) from Invivogen and morphine sulfate (#M1167) from Spectrum Chemicals.

Hauge Pedersen M., Pham J., Mancebo H., Inoue A., Asher W.B, & Javitch J.A. (2021). A novel luminescence-based β-arrestin recruitment assay for unmodified receptors. The Journal of Biological Chemistry, 296, 100503.

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Pharmacological Treatments and Irradiation

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Aripiprazole, quinpirole, and haloperidol were purchased from Tocris (Ellisville, MO, USA). A769662 was obtained from LC Laboratories (Woburn, MA, USA). Thioridazine was obtained from Cayman Chemical (Ann Arbor, MI, USA). Irradiation was performed at room temperature by using a ¹³⁷Cs gamma‐ray source Gammacell 3000 manufactured by Nordion (Ottawa, ON, Canada).

Lee H., Kang S., Sonn J.K, & Lim Y. (2019). Dopamine receptor D2 activation suppresses the radiosensitizing effect of aripiprazole via activation of AMPK. FEBS Open Bio, 9(9), 1580-1588.

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Dissecting Neurotransmitter Pathways

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6,7-dinitroquinoxaline-2,3-dione (DNQX) 10 μM, (2R)-amino-5-phosphonovaleric acid (APV) 50 μM (HelloBio), SR95531 hydrobromide (Gabazine) 10 μM (Tocris), Quinpirole 1 μM (Tocris), and RuBiGABA 10 μM (Tocris) were bath applied for at least 5 min before recording. For end of recording verification of GABA transmission, a saturating concentration of SR95531 (10 μM) was added directly into the recording chamber.

Lim S.A, & Surmeier D.J. (2021). Enhanced GABAergic Inhibition of Cholinergic Interneurons in the zQ175+/− Mouse Model of Huntington's Disease. Frontiers in Systems Neuroscience, 14, 626412.

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Comprehensive Pharmacological Toolkit Protocol

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All salts and all drugs not otherwise stated were from MilliporeSigma. Fluo5F and Alexa594 (Life Technologies), gabazine, d-AP5, hexamethonium chloride, oxotremorine M, GABA, and muscimol, were dissolved in deionized water. Sulpiride, quinpirole, picrotoxin, CGP55845 (Tocris), NBQX, and diazepam were dissolved in DMSO. Atropine was dissolved in DMSO and then diluted 1:10 in deionized water.

Kramer P.F., Twedell E.L., Shin J.H., Zhang R, & Khaliq Z.M. (2020). Axonal mechanisms mediating γ-aminobutyric acid receptor type A (GABA-A) inhibition of striatal dopamine release. eLife, 9, e55729.

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Preventing Dopamine Oxidation in Brain Slice Experiments

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Oscillatory activity was induced by bath application of CCh (5 μM) and KA (150 nM) and left to stabilise for at least 60 min prior to recording. Drugs were bath applied in known concentrations having been previously prepared in stock solutions of 1–50 mM and stored at -20°C. The drugs used were carbamoylcholine chloride (carbachol), DA and amphetamine (Sigma Ltd., Gillingham, UK), kainic acid (Abcam, Cambridge, UK), SKF38393, SCH23390, quinpirole, sulpiride, prazosin, phenylephrine (Tocris Bioscience, Bristol, UK). All drugs were applied for a minimum of 40 min before data were sampled.
DA is readily oxidised (observed with notable colour change) which will alter the effective concentration and potentially produce unreliable results. DA oxidation may also result in the production of reactive oxygen species and free radicals which may have a detrimental effect on slice viability. We found that the addition of the anti-oxidant ascorbic acid [38 (link),39 (link)] the aCSF prior to DA application, successfully prevented the oxidation of DA for the duration of our experiments. Baseline recordings for all experiments were therefore recorded in the presence of ascorbic acid (500 μM).

Özkan M., Johnson N.W., Sehirli U.S., Woodhall G.L, & Stanford I.M. (2017). Dopamine acting at D1-like, D2-like and α1-adrenergic receptors differentially modulates theta and gamma oscillatory activity in primary motor cortex. PLoS ONE, 12(7), e0181633.

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Pharmacological Manipulation of Neuronal Signaling

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Ghrelin (rat, mouse) was purchased from Phoenix Pharmaceuticals (Burlingame, CA). Tetrodotoxin (TTX), bicuculline (Bic), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and d-2-amino-5-phosphonopentanoic acid (AP5), quinpirole, SKF38393 and sulpiride were from Tocris Bioscience (Ellisville, MO). Dopamine was from Sigma-Aldrich (St. Louis, MO). Drugs were prepared and stored as stock solutions according to the manufacturer’s instructions and diluted in ACSF to obtain the experimental concentrations used in each experiment. All drug solutions were administered by a large-diameter (300 μm) flow pipe with the tip directed toward the recorded cell. During periods of no drug application, normal ACSF was continuously supplied to the recorded cell through the flow pipe.

Zhang X, & van den Pol A.N. (2016). Hypothalamic arcuate nucleus tyrosine hydroxylase neurons play orexigenic role in energy homeostasis. Nature neuroscience, 19(10), 1341-1347.

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Enkephalin and Opioid Receptor Pharmacology

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Drugs were applied by bath perfusion. The solution containing [Met⁵]enkephalin (ME) included the peptidase inhibitors, bestatin hydrochloride (10 μM) and thiorphan (1 μM). ME, [D-Ala², N-Me-Phe⁴, Gly⁵-ol]enkephalin (DAMGO), (+)-(5α,7α,8β)-N-Methyl-N-[7-(1-pyrrolidinyl)-1-oxaspiro[4.5]dec-8-yl]-benzeneacetamide (U69593), naloxone, picrotoxin, SR 95531, dopamine, sulpiride, 4-aminopyridine, DNQX, and tetrodotoxin (TTX) were obtained from Sigma-Aldrich (St. Louis, MO). MK-801 was purchased from Ascent Scientific (Weston-Super-Mare, UK). CGP55845, N-Cyclopentyladenosine (CPA), 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX) and quinpirole were obtained from Tocris Bioscience (Ellisville, MO).

Matsui A., Jarvie B.C., Robinson B.G., Hentges S.T, & Williams J.T. (2014). Separate GABA afferents to dopamine neurons mediate acute action of opioids, development of tolerance and expression of withdrawal. Neuron, 82(6), 1346-1356.

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Detailed Reagents and Materials for Cell Culture

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RPMI-1640, penicillin/streptomycin (P/S), 10X HEPES and 10X HBSS from Life Technologies (Carlsbad, CA). Human AB serum from Lonza (Basel, Switzerland). Fetal Bovine Serum from Lonza for MDM culture and from Gemini (West Sacramento, CA) for HEK293 culture. BSA, Acetylcholine, Probenecid, Dopamine, SKF81297, Sulpiride and SCH23390 from Sigma-Aldrich (St. Louis, MO). SKF38393 and Flupenthixol dihydrochloride from Tocris Biosciences (Minneapolis, MN). Quinpirole from Tocris or Sigma-Aldrich. All DR agonists and antagonists were resuspended in distilled H₂O. TAK779 was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH [41] (link). Macrophage colony stimulating factor (M-CSF) was from Peprotech (Rocky Hill, NJ), and was resuspended at 100 µM in distilled H₂O.

Gaskill P.J., Yano H.H., Kalpana G.V., Javitch J.A, & Berman J.W. (2014). Dopamine Receptor Activation Increases HIV Entry into Primary Human Macrophages. PLoS ONE, 9(9), e108232.

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Neurochemical Compounds for Functional Assays

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Apamin, iberiotoxin, cyclopiazonic acid, DHPG, DNQX, picrotoxin, quinpirole, SKF81297, SCH23390, CGS21680, and SCH58261 were obtained from Tocris Biosciences. TTX was obtained from Alomone Labs. Fluo-4FF and Alexa Fluor 594 were purchased from Life Technologies. Caged IP₃ was a generous gift from Dr. Kamran Khodakhah at Albert Einstein College of Medicine. All other chemicals were from Sigma-RBI.

Swapna I., Bondy B, & Morikawa H. (2016). Differential Dopamine Regulation of Ca2+ Signaling and Its Timing Dependence in the Nucleus Accumbens. Cell reports, 15(3), 563-573.

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