Before the end of the study, at weeks six and twelve, the mice were fasted for six hours in the morning. Under isoflurane anesthesia, blood was collected via cardiac punction, and euthanasia was performed by cervical dislocation. Blood samples were incubated for 30 min at room temperature and centrifuged for 15 min at 1500× g, and the obtained serum was immediately frozen and stored at −80 °C. Serum cholesterol and triglyceride levels as well as alanine aminotransferase (ALT) activity were measured by fluorometric assays (Abcam, Cambridge, UK). Serum insulin (Mercodia Ltd., Uppsala, Sweden), serum amyloid A (SAA), leptin, resistin, adipsin, and adiponectin (R&D Systems Europe Ltd., Abingdon, UK) were measured by enzyme-linked immunoassays (ELISAs). The detection limits were 33 pmol/L for insulin, 62.5 ng/L for SAA, 7.8 ng/L for leptin and resistin, 375 ng/L for adipsin, and 15.6 ng/L for adiponectin.
Serum insulin
Manufactured by Mercodia
Sourced in Sweden
Serum insulin is a laboratory assay kit designed to measure the concentration of insulin in human serum samples. It is a quantitative test that provides accurate and reliable results to support clinical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Adiponectin, by R&D Systems (2 mentions)
Adipsin, by R&D Systems (1 mentions)
Fluorometric assay, by Abcam (1 mentions)
Leptin, by R&D Systems (1 mentions)
Resistin, by R&D Systems (1 mentions)
Multiskan spectrum, by Thermo Fisher Scientific (1 mentions)
Adiponectin, by Adipogen (1 mentions)
Osteocalcin, by Takara Bio (1 mentions)
Lipocalin 2, by R&D Systems (1 mentions)
Bio plex pro mouse diabetes 8 plex, by Bio-Rad (1 mentions)
Igf 1, by R&D Systems (1 mentions)
Bio plex cytokine assay kit, by Bio-Rad (1 mentions)
Bio plex manager software, by Bio-Rad (1 mentions)
5 protocols using serum insulin
1
Fasting Blood Analysis in Mice
2
Multiparametric Metabolic Assessment
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Plasma glucose (QuantiChrom Glucose Assay Kit, Hayward, CA, USA), TG, TC (Boeringer mannheim, Germany), aspartate aminotransferase (AST), and the alanine aminotransferase (ALT) assay (IFCC, Human 12021, Wiesbaden, Germany) were measured by an absorbance reader (Multiskan spectrum, Thermo Scientific, Waltham, MA, USA). Commercially available ELISA kits were used for the measurement of plasma adiponectin (AdipoGen, Incheon, Korea) and serum insulin (Mercodia AB, Uppsala, Sweden).
3
Serum Biomarker Measurement Protocol
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The concentrations of triglycerides, NEFAs, and cholesterol were measured in freshly thawed samples of fasted serum using colorimetric enzymatic kits from WAKO Diagnostics (Osaka, Japan). Leptin concentrations were measured using a suspension bead array immunoassay (Bio-Plex Pro Mouse Diabetes 8-Plex) on a Bio-Plex series 100 instrument (both from Bio-Rad). Commercially available ELISAs were used for the measurement of serum insulin (Mercodia, Uppsala, Sweden), lipocalin-2 (R&D Systems, Minneapolis, MN, United States), and osteocalcin (Takara Bio, Mountain View, CA, U.S.A.). Serum concentrations of mouse tartrate-resistant acid phosphatase 5b (TRAP5b) and PINP were quantified using assays from Immunodiagnostic Services (Bolden, Tyne & Wear, U.K.). Liquid chromatography–tandem mass spectrometry was used to measure serum corticosterone levels.64 (link)
4
Biomarker Profiling of Metabolic Health
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The activities of serum alanine aminotransferase (ALT, EC 1.1.1.27) and aspartate aminotransferase (AST, EC 2.6.1.1) as well as levels of serum glucose, triglyceride, albumin, and total cholesterol were measured calorimetrically by the DRICHEM commercial assay system (Fujifilm, Tokyo, Japan). Serum insulin (Mercodia, Uppsala, Sweden), adiponectin and IGF-1 (both from R&D Systems, Minneapolis, MN, USA) were measured by commercial ELISA kits. Serum IL-1β, IL-12, and TNF-α were determined by the Bio-Plex cytokine assay kit in combination with the Bio-Plex Manager software (Bio-Rad, Hercules, CA, USA).
5
Metabolic Markers in Rats with Diabetic Diet
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Following 10–12 h of fasting, the rats were put into anesthesia using sodium pentobarbital and sufficient blood samples were taken from the eyes area. For accurate monitoring, samplings were performed in various stages (at the beginning, at the end of the 4th week of the HFD diet, 72 h after STZ injection, 48 h after exercise and at the end of the 4th week of de-training). To collect serum samples, blood samples were centrifuged at 2500 rpm for 15 min and stored at – 70 °C. Serum gel tubes were used to collect blood samples and microtubules were used for serum samples.
The concentrations of Leptin and TNF-α (Zellbio, GmbH, Germany- Diaclone, GmbH, and French), and serum insulin (Mercodia, Uppsala, Sweden) level were measured using ELISA method. The intra-assay coefficient of variation and sensitivity were 6.3%, 0.2 ng/Ml for leptin, 0.02 pg/Ml for TNF-α and 6.1%, 0.07 µg/l for insulin. The levels of FBG, LDL-C, and TG were measured by the colorimetric kits from Pars Azmoon Trading Company made in Iran. The intra-assay coefficient of variation and sensitivity for glucose, LDL-C, and TG were 1 mg/dl.
The concentrations of Leptin and TNF-α (Zellbio, GmbH, Germany- Diaclone, GmbH, and French), and serum insulin (Mercodia, Uppsala, Sweden) level were measured using ELISA method. The intra-assay coefficient of variation and sensitivity were 6.3%, 0.2 ng/Ml for leptin, 0.02 pg/Ml for TNF-α and 6.1%, 0.07 µg/l for insulin. The levels of FBG, LDL-C, and TG were measured by the colorimetric kits from Pars Azmoon Trading Company made in Iran. The intra-assay coefficient of variation and sensitivity for glucose, LDL-C, and TG were 1 mg/dl.
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