Mouse monoclonal antibody against light chain lrp1

Amylin (human, Cat# AS-60254-1) and AC253 were purchased from AnaSpec (Fremont, CA). Acetyl-[Asn30, Tyr32] sCT(8–37) (AC187) was purchased from Tocris Bioscience (Minneapolis, MN). Synthetic monoiodinated and nonoxidized Aβ₄₀ (¹²⁵I-Aβ₄₀; human, 2200 Ci/mmol) was purchased from PerkinElmer (Boston, MA). Fetal bovine serum (FBS) was purchased from ATLANTA biological (Flowery Branch, GA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Invitrogen (Carlsbad, CA). Bovine serum albumin (BSA) was purchased from Sigma-Aldrich (St. Louis, MO). Total protein measurement’s reagents with the bicinchoninic acid (BCA) method were obtained from Pierce (Rockford, IL). For western blot, mouse monoclonal antibody against light chain LRP1 was obtained from Abcam (Cambridge, MA), CAT# ab92544; goat polyclonal antibodies against actin (C-11), CAT# sc-1615, and HRP-labeled secondary antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). All other chemicals and reagents were of analytical grade and were readily available from commercial sources.

Bovine serum albumin (BSA) was purchased from Sigma-Aldrich (St. Louis, MO). Rivastigmine L-tartrate was obtained from Tokyo Chemical Industry Co., LTD (TCI; Portland, OR). Complete mammalian protease inhibitor, donkey serum, Ficoll 400, and sodium dodecylphasphate (SDS) were obtained from (Sigma-Aldrich, MO). Reagents for total protein analysis with the bicinchoninic acid method were obtained from Pierce (Rockford, IL). For Western blot and immunohistochemistry, the mouse monoclonal antibody C-219 against P-gp was obtained from Covance Research Products (Dedham, MA); mouse monoclonal antibody against light chain LRP1 was obtained from Abcam (Cambridge, MA); goat polyclonal antibodies against actin (C-11), HRP-labeled secondary antibodies, rabbit polyclonal anti-GFAP antibody, rabbit polyclonal anti-EAAT (GLT-1) antibody, and CFL594-conjugated donkey anti-rabbit IgG were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Mouse monoclonal anti-PSD-95 antibody and rabbit polyclonal anti-SNAP-25 antibody were obtained from GeneTex, Inc (Irvine, CA). All other chemicals and reagents were of analytical grade and were readily available from commercial sources.

Fibronectin from bovine plasma and Lucifer Yellow (LY) were purchased from Sigma-Aldrich (St. Louis, MO). HTS 3μm polycarbonate membrane transwell 96-well plates and 0.45 μm polyester membrane transwell 24 well plates were purchased from Corning (Corning, NY). Rat-tail collagen type-I, Dulbecco’s modi ed Eagle’s medium (DMEM), sterile PBS, and penicillin/streptomycin antibiotics were obtained from Gibco (Grand Island, NY). Fetal bovine serum (FBS) was purchased from Atlanta biologicals (Flowery Branch, GA). ¹⁴C-inulin ([carboxyl-¹⁴C]-inulin, M.W. 5000 Da) was purchased from American Radiolabeled Chemicals (St. Louis, MO). Synthetic mono-iodinated and nonoxidized Aβ₄₀ (¹²⁵I-Aβ₄₀; human, 2200 Ci/mmol) was purchased from PerkinElmer (Boston, MA). Total protein measurement’s reagents with the bicinchoninic acid (BCA) method were obtained from Pierce (Rockford, IL). Antibodies used were mouse monoclonal antibody against light chain LRP1 (Abcam, Cambridge, MA), mouse monoclonal antibody C-219 against P-gp from BioLegend (San Diego, CA), monoclonal antibody for claudin-5 and ZO-1 were purchased from Invitrogen (Carlsbad, CA), goat polyclonal antibodies against actin (C-11), and HRP-labeled secondary antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX). All other chemicals, reagents and supplies were purchased from VWR (West Chester, PA) and Sigma-Aldrich.