The GPC analysis was performed at Toray Research Center (Tokyo, Japan). The purified sample (1 mg) was dissolved in 1 mL of dimethyl formamide (DMF) containing 50 mM lithium chloride (LiCl), and 200 μL of the sample was subjected to GPC. The molecular mass was estimated using a high-performance liquid chromatograph equipped with a refractive index detector (RI-8020; TOSOH, Tokyo, Japan), and TSK gel α-4000 and TSK gel α-2500 columns (7.8 mm × 30 cm; TOSOH, Tokyo, Japan), which were connected directly. DMF containing 50 mM LiCl was used as the solvent at a flow rate of 0.7 mL/min and the column temperature was maintained at 23 °C. The eluted sample was used for molecular mass determination, with a calibration curve obtained using standard polystyrene kits (PSt Quick E and F; TOSOH, Tokyo, Japan).
Tsk gel α 2500
Manufactured by Tosoh
Sourced in Japan
The TSK gel α-2500 is a size exclusion chromatography column designed for the separation and analysis of macromolecules. It utilizes a porous polymer gel to facilitate the separation of samples based on their molecular size and shape.
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Lab products found in correlation
3 protocols using tsk gel α 2500
1
Gel Permeation Chromatography Analysis
2
Gel Permeation Chromatography Analysis
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The GPC analysis was performed at Toray Research Center (Tokyo, Japan). The puri ed sample (1 mg) was dissolved in 1 mL of dimethyl formamide (DMF) containing 50 mM lithium chloride (LiCl), and 200 μL of the sample was subjected to GPC. The molecular mass was estimated using a high-performance liquid chromatograph equipped with a refractive index detector (RI-8020; TOSOH, Tokyo, Japan), and TSK gel α-4000 and TSK gel α-2500 columns (7.8 mm × 30 cm; TOSOH, Tokyo, Japan), which were connected directly. DMF containing 50 mM LiCl was used as the solvent at a ow rate of 0.7 mL/min and the column temperature was maintained at 23°C. The eluted sample was used for molecular mass determination, with a calibration curve obtained using standard polystyrene kits (PSt Quick E and F; TOSOH, Tokyo, Japan).
3
GPC Analysis of Polymer Molecular Mass
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The GPC analysis was performed by Torey Research Center (Japan). The puri ed sample (1 mg) was dissolved in 1 mL of dimethyl formamide (DMF) containing 50 mM lithium chloride, and 200 μL of the sample was subjected to GPC. The molecular mass was estimated using a high-performance liquid chromatograph equipped with a refractive index detector (RI-8020; TOSOH, Japan), and TSK gel α-4000 and TSK gel α-2500 columns (7.8 mm × 30 cm; TOSOH), which were connected directly. DMF containing 50 mM lithium chloride was used as the solvent at a ow rate of 0.7 mL/min and the column temperature was maintained at 23°C. The eluted sample was used for molecular mass determination, with a calibration curve obtained using standard polystyrene kits (PSt Quick E and F; TOSOH). 13
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