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Anti ligase 4

Manufactured by Thermo Fisher Scientific
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Anti-Ligase IV is a lab equipment product that functions as an inhibitor of the DNA repair enzyme Ligase IV. It is used in research applications to study the role of Ligase IV in DNA repair processes.

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Lab products found in correlation

Anti xrcc4, by Abcam (2 mentions) Anti xlf, by Abcam (2 mentions) Anti gfp, by Thermo Fisher Scientific (1 mentions) Anti flag m2, by Merck Group (1 mentions) Anti gapdh, by Proteintech (1 mentions) Envision detection kit, by Agilent Technologies (1 mentions)

2 protocols using anti ligase 4

1

Whole Cell Protein Extraction and Analysis

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Whole cell extracts were obtained using NETN lysis buffer. The proteins were separated by 10% SDS-PAGE and detected using the following primary antibodies according to the manufacturer's instructions: anti-XRCC4 (Abcam, MA), anti-XLF (Abcam, MA), anti-Ligase IV (Thermo Scientific), anti-FLAG (M2, Sigma-Aldrich), anti-GFP (Invitrogen), and anti-GAPDH (Proteintech).
He M., Hu X., Chen L., Cao A.Y., Yu K.D., Shi T.Y., Kuang X.Y., Shi W.B., Ling H., Li S., Qiao F., Yao L., Wei Q., Di G.H, & Shao Z.M. (2014). A recessive variant of XRCC4 predisposes to non-BRCA1/2 breast cancer in chinese women and impairs the DNA damage response via dysregulated nuclear localization. Oncotarget, 5(23), 12218-12232.
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2

Immunohistochemical Localization of DNA Repair Proteins in Breast Tumors

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Paraffin breast tumor sections were subjected to immunohistochemical staining to ascertain the protein localization of XRCC4, XLF, and Ligase IV. The tumor sections were incubated with the following primary antibodies: anti-XRCC4 (1:500; Abcam, MA), anti-XLF (1:400; Abcam, MA), and anti-Ligase IV (1:600; Thermo Scientific). HRP-conjugated secondary antibodies were used, followed by colorimetric detection using an Envision detection kit (DAKO, Japan).
He M., Hu X., Chen L., Cao A.Y., Yu K.D., Shi T.Y., Kuang X.Y., Shi W.B., Ling H., Li S., Qiao F., Yao L., Wei Q., Di G.H, & Shao Z.M. (2014). A recessive variant of XRCC4 predisposes to non-BRCA1/2 breast cancer in chinese women and impairs the DNA damage response via dysregulated nuclear localization. Oncotarget, 5(23), 12218-12232.
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