Aminoguanidine carbonate

Glyceryl oleate (Tegin O^®, GO), Cetyl palmitate (CP), Methylisothiazolinone and Methylchloroisothiazolinone (Acnibio AC^®) and triethanolamine (TEA) were bought from ACEF (Fiorenzuola D’Arda, Italy). Idebenone (IDE) was obtained from Carbosynth (Berkshire, UK). Polyoxyethylene-20-oleyl ether (Brij 98^®, Oleth-20), 2,2’-Azobis(2-methylpropionamidine) dihydrochloride (AAPH), fluorescin (FL) Trolox (TRL), aminoguanidine carbonate (AMG), bovine serum albumin (BSA), D-fructose, sodium nitroprusside and Griess reagent (1% dihydrochloride sulphanylamide and 0.1% naphthylethylenediamine (NED) dihydrochloride in 5% of hydrochloric acid) and sodium azide were purchased from Sigma-Aldrich srl (Milan, Italy). Curcumin was obtained from MP Biomedicals, (Illkirch cedex, France). Imidazolidinyl urea (Kemipur 100) was obtained from Farmalabor (Canosa di Puglia, Italy).
The following chemicals 2-Pyrrolidone-5-carboxylic acid (PCA), N,N′-dicyclohexylcarbodiimide (DCC), dry acetonitrile (CH₃CN), 4-dimethylaminopyridine (DMAP), ethyl acetate (EtOAc), and hydrochloric acid (HCl), used to synthesize IDE ester (IDEPCA), were reagent grade and were bought from Sigma-Aldrich srl (Milan, Italy). DMSO-d₆ used for NMR analysis was purchased from Sigma-Aldrich srl (Milan, Italy).

Ethanol, 2,2’-Azobis(2-methylpropionamidine) dihydrochloride (AAPH), fluorescein (FL), 6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), aminoguanidine carbonate (AMG), bovine serum albumin (BSA), d-fructose, sodium nitroprusside and Griess reagent (1% dihydrochloride sulphanylamide and 0.1% naphthylethylenediamine (NED) dihydrochloride in 5% of hydrochloric acid), sodium azide, curcumin and analytical standards were purchased from Sigma-Aldrich srl (Milan, Italy). Water LC-MS Grade LiChrosolv and formic acid were obtained from Merck (Milan, Italy), acetonitrile gradient grade for high-performance liquid chromatography (HPLC) HiPerSolv Chromanorm from VWR Chemicals (Milan, Italy).

The following chemicals and solvents used to synthesize and characterize RSVTR were reagent grade and were bought from Sigma-Aldrich S.r.l. (Milan, Italy): resveratrol (RSV), trolox, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC. HCl), 4-dimethylaminopyridine (DMAP), dry dichloromethane (DCM), cyclohexane, ethyl acetate (EtOAc), hydrochloric acid (HCl), and DMSO-d₆ used for NMR analysis.
Moreover, 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH), fluorescein (FL), amino-guanidine carbonate (AMG), bovine serum albumin (BSA), D-fructose, sodium azide, triethylamine, and trifluoroacetic acid were purchased from Sigma-Aldrich S.r.l. (Milan, Italy). Acetonitrile and water of HPLC grade were obtained from Merck (Darmstadt, Germany).