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Oil red o

Manufactured by Bio Basic
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Sourced in Canada, United States

Oil Red O is a lipid-soluble diazo dye used for the staining of neutral triglycerides and lipids in frozen sections and cell samples. It has a maximum absorption at 518 nm and can be used for the identification and visualization of lipid-rich structures.

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Lab products found in correlation

Lysophosphatidic acid, by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions) Sodium palmitate, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole dihydrochloride dapi, by Santa Cruz Biotechnology (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions) Microplate reader, by Agilent Technologies (1 mentions)

2 protocols using oil red o

1

Lipid Accumulation Measurement Assay

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Roswell Park Memorial Institute-1640 (RPMI-1640) medium was obtained from Gibco (Grand Island, NY, USA). Oil red O was purchased from Bio Basic Inc. (Markham, ON, Canada). 4′6-diamidino-2-phenylindole dihydrochloride (DAPI) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Oxidized low density lipoprotein (ox-LDL) was obtained from Yi Yuan Biotechnologies (Guangzhou, China). The following reagents were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA): sodium palmitate (PA), phorbol 12-myristate 13-acetate (PMA), rapamycin, lysophosphatidic acid (LPA), polyvinyl alcohol mounting medium with DABCO (PVA-DABCO; Sigma-Aldrich).
Zheng H., Fu Y., Huang Y., Zheng X., Yu W, & Wang W. (2017). mTOR signaling promotes foam cell formation and inhibits foam cell egress through suppressing the SIRT1 signaling pathway. Molecular Medicine Reports, 16(3), 3315-3323.
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2

Lipid Staining and Quantification

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After fixation with formaldehyde, neutral lipids were stained using 0.5% Oil Red O (Bio Basic Inc., Amherst, NY, USA) in isopropanol for 1 min. After removing the staining solution, the OD was measured at 500 nm using a microplate reader (BioTek).
Chen Y.C., Chen H.J., Huang B.M., Chen Y.C, & Chang C.F. (2019). Polyphenol-Rich Extracts from Toona sinensis Bark and Fruit Ameliorate Free Fatty Acid-Induced Lipogenesis through AMPK and LC3 Pathways. Journal of Clinical Medicine, 8(10), 1664.
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