Agilent 6400 ultra high performance liquid chromatograph with tripe qquadrupole mass spectrometer uhplc qqq ms ms

Manufactured by Agilent Technologies

The Agilent 6400 Ultra High Performance Liquid Chromatograph with Triple Quadrupole Mass Spectrometer (UHPLC-QqQ MS/MS) is a highly sensitive and versatile analytical instrument designed for the separation, detection, and quantification of a wide range of chemical compounds. It combines the high resolution and speed of UHPLC with the accurate mass analysis and tandem MS/MS capabilities of a triple quadrupole mass spectrometer.

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Lab products found in correlation

Methanol, by Merck Group (2 mentions) Flag m purification kit, by Merck Group (1 mentions)

Close spelling variants of this product

High-performance liquid chromatograph MS QQQ mass spectrometer UHPLC-QQQ-MS/MS UHPLC-QQQ-MS Agilent 6400 Liquid chromatograph UHPLC-MS/MS Spectrometers

2 protocols using agilent 6400 ultra high performance liquid chromatograph with tripe qquadrupole mass spectrometer uhplc qqq ms ms

Quantification of Chalcone Derivatives in Cells and Bone

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To determine in vitro cellular uptake, interaction of small molecules with Smurf1, bone distribution and osteoblast accumulation chalcone derivative formulations, small molecules in immunoprecipitates or chalcone derivative formulations in osteoblasts or bone extraction were extracted by 90% methanol (Sigma) and analyzed by Agilent 6400 Ultra High Performance Liquid Chromatograph with Tripe Qquadrupole Mass Spectrometer (UHPLC-QqQ MS/MS) (Agilent Technologies)^{54 (link)}.

Liang C., Peng S., Li J., Lu J., Guan D., Jiang F., Lu C., Li F., He X., Zhu H., Au D.W., Yang D., Zhang B.T., Lu A, & Zhang G. (2018). Inhibition of osteoblastic Smurf1 promotes bone formation in mouse models of distinctive age-related osteoporosis. Nature Communications, 9, 3428.

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Assessing AHR-Leflunomide Direct Interaction

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To determine the direct interaction of AHR with Leflunomide, 30.0 μg Flag-tagged AHR and AHR mutants in 100 μL PBS was incubated with Leflunomide (10.0 μM), A77 1726 (10.0 μM) and vehicle (DMSO) for 18 h at 4 °C, respectively. Flag-tagged proteins were collected by a FLAG® M purification kit (Sigma-Aldrich). The small molecules in pull-down assays were extracted by 90% methanol (Sigma-Aldrich) and analyzed by Agilent 6400 Ultra High Performance Liquid Chromatograph with Tripe Qquadrupole Mass Spectrometer (UHPLC-QqQ MS/MS) (Agilent Technologies)^{28 (link),68 (link)}.

Liang C., Li J., Lu C., Xie D., Liu J., Zhong C., Wu X., Dai R., Zhang H., Guan D., Guo B., He B., Li F., He X., Zhang W., Zhang B.T., Zhang G, & Lu A. (2019). HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis. Nature Communications, 10, 4579.

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