Microtiter plates were coated with recombinant ICAM-1–Fc (50 μl, 2 μg/ml; glycine/HCl buffer; overnight; 4°C) and blocked with blocking buffer. DBLβ3_D4 domains (1–16 μg/ml) were added simultaneously with mAb 24E9 added in 2-fold dilutions ranging from 0.25 to 32 μg/ml. Mouse IgG (Life Technologies) was added as control. ICAM-1–bound DBLβ3_D4 was detected using anti–penta-His HRP Ab (1:3000 in blocking buffer; 1 h; room temperature; QIAGEN). Washing and detection were performed as described above.
Anti penta his hrp ab
Manufactured by Qiagen
The Anti–penta-His HRP Ab is a horseradish peroxidase (HRP)-conjugated antibody that specifically binds to the penta-histidine (penta-His) tag. It is used to detect and visualize recombinant proteins that have been tagged with a penta-His sequence.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti penta his hrp ab
1
ICAM-1 Binding Assay with DBLβ3_D4
2
Binding assay for Plasmodium falciparum PFD1235w
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Microtiter plates were coated (50 μl; 2-fold dilutions starting at 64 μg/ml; glycine/HCl buffer; overnight; 4°C) with 24E9 mAb or the PFD1235w DBLγ-specific AB01 mAb (24 (link)) and blocked with PBS + 1% BSA. Plates were washed in PBS and PFD1235w DBLβ3_D4 or PFD1235w DBLγ (50 μl; 2 μg/ml; PBS ± 50 mM DTT; 1 h; room temperature) were added to the plates coated with 24E9 or AB01, respectively. Bound DBLβ3_D4 or DBLγ was detected by use of an anti–penta-His HRP Ab (1:3000 in PBS + 1% BSA; 1 h; room temperature; QIAGEN). Washing and detection were performed as described above.
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