Isofraxidin

Manufactured by Merck Group

Sourced in Germany, United States

Isofraxidin is a chemical compound used as a laboratory standard and analytical reference material. It is a naturally occurring coumarin derivative that can be isolated from various plant sources. Isofraxidin is commonly used in analytical and research applications, but its specific core function is to serve as a reference standard for identification and quantification purposes in chemical analysis.

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Lab products found in correlation

Esculin, by Merck Group (1 mentions) Esculetin, by Merck Group (1 mentions) Scopoletin, by Merck Group (1 mentions) Fraxetin, by Merck Group (1 mentions) Fraxin, by Merck Group (1 mentions) Scopolin, by Merck Group (1 mentions) 4 methylumbelliferon, by Merck Group (1 mentions) Silyl 991, by Macherey-Nagel (1 mentions) Methoxyamine hcl, by Merck Group (1 mentions)

2 protocols using isofraxidin

Quantitative Metabolite Analysis via LC-MS

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All buffer constituents and solvents were of reagent or HPLC grade and obtained from either Roth (Karlsruhe, Germany), Sigma-Aldrich (St Louis, MO, USA) or J. T. Baker (Deventer, The Netherlands). Coumarin standards were purchased from PhytoLab (Vestenbergsgreuth, Germany; scopolin, isofraxidin, fraxin), and Sigma-Aldrich (esculin, esculetin, fraxetin, 4-methyl-umbelliferon, scopoletin). [2,2,4,4-²H]citric acid and [2,3,3-²H]malic acid were from CDN isotopes (Point Claire, QC, Canada), [2,2,3,3-²H]succinic acid and methoxyamine HCl from Sigma-Aldrich, and Silyl-991 from Macherey-Nagel (Düren, Germany).

Ziegler J., Schmidt S., Chutia R., Müller J., Böttcher C., Strehmel N., Scheel D, & Abel S. (2015). Non-targeted profiling of semi-polar metabolites in Arabidopsis root exudates uncovers a role for coumarin secretion and lignification during the local response to phosphate limitation. Journal of Experimental Botany, 67(5), 1421-1432.

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Isofraxidin Cytotoxicity Evaluation in BMMs

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BMMs (5 × 10⁴ cells) were seeded into 96-well plates and incubated for 16 h in media as mentioned in Cell Culture section. Various concentrations (0, 6.25, 12.5, and 25 µM) of isofraxidin (Sigma-Aldrich, St. Louis, MO, USA) were added to cells and maintained for 24 h at 37°C in a 95% air and 5% CO₂ atmosphere incubator. After that, 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium (MTT) was added and incubated for 2 h. Finally, BMMs were treated with 200 µl of dimethyl sulfoxide and the absorbance was quantified at 490 nm wavelength using a microplate reader (Bio-Tek).

Wang W, & Wang B. (2021). Isofraxidin Inhibits Receptor Activator of Nuclear Factor-κB Ligand–Induced Osteoclastogenesis in Bone Marrow–Derived Macrophages Isolated from Sprague–Dawley Rats by Regulating NF-κB/NFATc1 and Akt/NFATc1 Signaling Pathways. Cell Transplantation, 30, 0963689721990321.

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