Active GLP-1, total GIP, and total ghrelin were analyzed using ELISAs from Millipore (Darmstadt, Germany). Insulin was analyzed using an ELISA from Mercodia (Uppsala, Sweden), and glucose was analyzed using a commercially available kit (Infinity Glucose Oxidase) from Thermo Fisher Scientific (Lexington, MA, USA). Assays were performed according to the instructions provided by the manufacturers.
Infinity glucose oxidase
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Infinity Glucose Oxidase is a laboratory product designed to measure glucose levels. It utilizes the glucose oxidase enzyme to facilitate the oxidation of glucose and produce hydrogen peroxide, which is then detected to determine the glucose concentration.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using infinity glucose oxidase
1
Comprehensive Metabolic Analysis Protocol
2
Quantification of Glucose and Insulin
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glucose was quantified by a glucose oxidase activity assay. Briefly, glucose standards were diluted by serial dilution and standards, controls (glucose, Sigma-Aldrich), and samples were aliquoted into a 96-well plate in duplicate. glucose oxidase (Infinity glucose Oxidase, Thermo Scientific, Altham, MA, USA) was then aliquoted into each well. The plate was then shaken for 30 seconds and incubated for 10 minutes at 37°C. The plate was read on a plate reader (BioRad iMark, Herculus, CA, USA) with a 540 nm filter.
Insulin was quantified by enzyme-linked immunosorbent assay (ELISA, Mercodia, Uppsala, Sweden) per the instructions on the aforementioned plate reader.
Insulin was quantified by enzyme-linked immunosorbent assay (ELISA, Mercodia, Uppsala, Sweden) per the instructions on the aforementioned plate reader.
3
Glucose Homeostasis in β-Tfb2m Mice
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Blood was collected when the mice were killed by cervical dislocation and decapitation at d18, d50 (β-Tfb2m−/−), and seven months of age (β-Tfb2m+/−). Quantitative determination of plasma glucose was carried out using Infinity™ Glucose Oxidase (Thermo Fisher Scientific, (Waltham, MA, USA). Insulin was measured by enzyme-linked immunosorbent assay (ELISA) (Mercodia, Uppsala, Sweden). Intravenous glucose tolerance test (IVGTT) was also carried out on β-Tfb2m+/− mice at six months of age as described [15] (link). Food was removed 1 h before the glucose challenge. The mice were anesthetized as previously described [15] (link). Blood was collected retro-orbitally at 0, 1, 5, 10, 20, 50 and 75 min after intravenous injection of glucose (1 g/kg body weight) for determination of plasma glucose and insulin concentration as described above.
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