Hematoxylin and eosin

Manufactured by Roche

Sourced in Germany

Hematoxylin and eosin is a staining system used in histology, the branch of anatomy that studies the microscopic structure of tissues. This staining technique is used to differentiate and visualize various cellular and tissue components under a microscope. Hematoxylin stains nuclei blue, while eosin stains cytoplasm and other structures pink or red, providing a contrast that allows for the identification of different cell types and tissue structures.

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Lab products found in correlation

Phosphate buffer saline tablet, by Merck Group (1 mentions) L lactide, by Merck Group (1 mentions) Tin 2 2 ethylhexanoate, by Merck Group (1 mentions) ε caprolactone, by Merck Group (1 mentions) Ventana benchmark xt, by Roche (1 mentions) Ab27588, by Abcam (1 mentions) Ab135372, by Abcam (1 mentions) Ab178089, by Abcam (1 mentions) Axio scan z1, by Zeiss (1 mentions) Am33034pu n, by OriGene (1 mentions) Brown norway rat, by Charles River Laboratories (1 mentions) Envision system hrp labelled polymer anti rabbit, by Agilent Technologies (1 mentions) Target retrieval system, by Agilent Technologies (1 mentions) Polyclonal goat anti rabbit hrp, by Agilent Technologies (1 mentions) Liquid dab substrate chromogen system, by Agilent Technologies (1 mentions) Autostainer plus, by Agilent Technologies (1 mentions) Optiview universal dab detection kit, by Roche (1 mentions) Nkcc2, by StressMarq Biosciences (1 mentions) Red 610 kit, by Roche (1 mentions) Pdgfra, by Roche (1 mentions)

6 protocols using hematoxylin and eosin

Bioresorbable Polymer Material Synthesis

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We purchased L-lactide, ε-caprolactone, Tin(II) 2-ethylhexanoate, phosphate buffer saline (PBS) tablets from Sigma-Aldrich, Czech Republic, paraffin (histowax 56–58°C) from Bamed s.r.o., Czech Republic, hematoxylin and eosin from Roche s.r.o., Czech Republic, anti-CD31(cluster of differentiation 31, or also known as platelet endothelial cell adhesion molecule-1) antibody from Acris Antibody GmbH, Germany. All other reagents and chemicals were obtained from P-Lab a.s., Lach-Ner s.r.o. and Sigma-Aldrich Czech Republic, and were used as received.

Kasoju N., Kubies D., Kumorek M.M., Kříž J., Fábryová E., Machová L., Kovářová J, & Rypáček F. (2014). Dip TIPS as a Facile and Versatile Method for Fabrication of Polymer Foams with Controlled Shape, Size and Pore Architecture for Bioengineering Applications. PLoS ONE, 9(10), e108792.

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Histochemical and Ultrastructural Analysis of Mouse Kidney

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Histochemistry was performed as previously described^{8 (link),39 (link)} with some modifications. Four-micrometer sections of the paraffin blocks of mouse kidney tissues were cut and attached on coated slides using an automatic immunostaining machine (Ventana Benchmark XT, Roche Diagnostics, Basel, Switzerland). The sections were stained with hematoxylin and eosin (Roche Diagnostics). TEM was performed as previously described^{8 (link)}. The TEM images were analyzed by a renal pathologist in a blinded manner and results were analyzed using one- or two-way ANOVA followed by Dunnett’s or Tukey’s multiple comparison tests.

Kim J.H., Hwang K.H., Dang B.T., Eom M., Kong I.D., Gwack Y., Yu S., Gee H.Y., Birnbaumer L., Park K.S, & Cha S.K. (2021). Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nature Communications, 12, 6537.

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Multiplex Immunofluorescence Analysis of Tumor Microenvironment

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We used 38 pretreatment samples from the MSI pembrolizumab cohort for B2M staining (ab27588; Abcam) and MHC class I (HCA2, AM33034PU-N; Acris) immunofluorescence staining. Of the 38 samples, 20 specimens underwent an additional multiplex immunofluorescence panel with primary antibodies against CD8 (clone SP239, ab178089; Abcam), pan-cytokeratin (760-2595; Ventana), CD3 (clone SP162, ab135372; Abcam), and PD-L1 (clone SP263, 790-4905; Ventana). All staining procedures were automated on the BenchMark ULTRA automated slide stainer and scanned on the Zeiss AxioScan Z1. Slides were also stained with hematoxylin and eosin (Roche) and scanned using the iScanHT to annotate regions of interest. Image analysis was integrated within Roche Digital Pathology software to quantitate cell densities and characterize the tumor microenvironment.

Germano G., Lu S., Rospo G., Lamba S., Rousseau B., Fanelli S., Stenech D., Le D.T., Hays J., Totaro M.G., Amodio V., Chilà R., Mondino A., Diaz LA J.r., Di Nicolantonio F, & Bardelli A. (2021). CD4 T Cell-Dependent Rejection of Beta-2 Microglobulin Null Mismatch Repair-Deficient Tumors. Cancer discovery, 11(7).

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Synthesis and Characterization of PLCL Scaffolds

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High molecular weight poly(L-lactide-co-εcaprolactone) (PLCL, M w = 316 000 g/mol and M n = 120 000 g/mol) were synthesized by ring-opening co-polymerization of the corresponding monomers (L-lactide, ε-caprolactone) in the presence of Tin(II) 2-ethylhexanoate as a catalyst in bulk as previously described (Kubies et al. 2000) . Capsules based on a nonabsorbable Polyamide monofilament based mesh were purchased from ELLA-CS, Czech Republic, and were used as the control capsules. We purchased hematoxylin and eosin from Roche s.r.o., Czech Republic, rabbit anti-CD31 and PECAM1 (cluster of differentiation 31 and platelet endothelial cell adhesion molecule-1) polyclonal antibody from Acris Antibody GmbH, Germany, biotinylated goat anti-rabbit IgG antibody (H+L) included in Vectastain Elite ABC Reagent, R.T.U. (Ready-to-Use) kit from Vector Laboratories, California, USA, and paraffin (histowax 56-58 °C) from Bamed s.r.o., Czech Republic. All other reagents and chemicals, unless otherwise stated, were obtained from P-Lab a.s., Lach-Ner s.r.o. and Sigma-Aldrich Czech Republic, and were used as received. Brown Norway rats (male, 230-270 g, aged between 2-3 months) were purchased from Charles River, Germany.

Kasoju N., Kubies D., Fábryová E., Kříž J., Kumorek M.M., Sticová E, & Rypáček F. (2015). In vivo vascularization of anisotropic channeled porous polylactide-based capsules for islet transplantation: the effects of scaffold architecture and implantation site. Physiological research, 64(Suppl 1).

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Immunohistochemical Analysis of Bone Markers

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5-micrometer sections of FFPE tissue were mounted on charged slides, stained with Hematoxylin and Eosin (Ventana Symphony). For immunohistochemistry, antigen retrieval was performed on the PT Link platform on the Dako Autostainer Plus instrument or manually using Dako Target Retrieval System citrate buffer. Following blocking, tissue was incubated with SP7 Osterix antibody (Abcam, 1:2000) or MTCO2 antibody (Abcam 1:800), washed and then secondary antibody (Polyclonal Goat Anti-Rabbit HRP or Envision+System HRP labelled polymer Anti-Rabbit, Dako 1:100) and developed with Dako Liquid DAB+ Substrate Chromogen System. Collagen staining was performed via Picro Sirius Red Stain Kit (Connective Tissue Stain, Abcam).

Harlow M.L., Chasse M.H., Boguslawski E.A., Sorensen K.M., Gedminas J.M., Kitchen-Goosen S.M., Rothbart S.B., Taslim C., Lessnick S.L., Peck A.S., Madaj Z.B., Bowman M.J, & Grohar P.J. (2019). Trabectedin Inhibits EWS-FLI1 and Evicts SWI/SNF from Chromatin in a Schedule Dependent Manner. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(11), 3417-3429.

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Immunohistochemical Profiling of Organoid Structures

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Four-micron sections of formalin-fixed paraffin embedded organoids were stained with hematoxylin and eosin (Ventana Medical Systems, Tucson, AZ). Immunohistochemistry was performed with an automated, validated, and accredited staining system (Ventana Benchmark ULTRA) using the UltraView or OptiView universal DAB detection Kit (Ventana Medical Systems). After deparaffinization and heat-induced antigen retrieval, the tissue samples were incubated with WT1 (Cell Marque, Rocklin, CA), ECAD (Ventana Medical Systems), Villin-1 (Abcam, Cambridge, UK), CD31 (Cell Marque), renin (Abcam), NKCC2 (StressMarq Biosciences, Victoria, BC, Canada), NHE3 (StressMarq), CD34 (Cell Marque), COL1A1 (Novus Biologicals, Littleton, CO), or PDGFRa (R&D systems). Incubation was followed by hematoxylin II counterstaining. Double stainings of PDGFRa and renin were performed by incubating the samples with PDGFRa for 32 minutes at 37 C followed by detection with Red610 kit (#760-245, Ventana) and renin for 32 minutes at 37 C followed by detection with FAM kit (#760-243, Ventana). An AF488-conjugated anti-human mitochondria antibody MAB1273A4 (Millipore, Billerica, MA) was used to detect

Shankar A.S., Du Z., Mora H.T., Bosch T.v.d., Korevaar S.S., Garrelds I.M., Bresnick E., Iglesias C., Groningen M.C.v., Gribnau J., Baan C.C., Danser J., Hoorn E.J., & Hoogduijn M.J. (2020). Human kidney organoids produce functional renin. Cytotherapy, 22(5), S10-S11.

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