ChIP assays were performed using EZ-CHIP KIT according to the manufacturer’s instructions (Millipore, USA). EZH2 was obtained from Abcam. H3 trimethyl Lys 27 antibody, Histone H3, and Acetyl-Histone H3 Lys27 were from Millipore. The ChIP primer sequences are listed in Table S2 . Quantification of immunoprecipitated DNA was performed using qPCR. ChIP data were calculated as a percentage relative to the input DNA by the following equation: 2[Input Ct − Target Ct] × 100 (%).
Acetyl histone h3 lys27
Manufactured by Merck Group
Acetyl-Histone H3 Lys27 is a laboratory reagent that detects the acetylation of lysine 27 on histone H3 protein. It is used as a tool for epigenetic research and analysis.
Automatically generated - may contain errors
4 protocols using acetyl histone h3 lys27
1
ChIP Assay Using EZ-CHIP Kit
2
Chromatin Immunoprecipitation Assay for EZH2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EZ-CHIP KIT (Millipore, USA) was used to perform the CHIP assay. EZH2 was provided by Abcam and the H3 trimethyl Lys 27 antibody, Histone H3, and Acetyl-Histone H3 Lys27 were provided by Millipore. The ChIP primer sequences were: KLF2 (Forward) 5′- ACGGGCTTATTGAGGTTGG-3′ and KLF2 (Reverse) 5′- GCCTGGGTGACAGAGGAGAC-3′. The immunoprecipitated DNA was quantitated by qPCR. The formula, 2[Input Ct − Target Ct] × 100 (%), was used to interpret the raw data. Each test was conducted independently in triplicate.
3
ChIP Assay Protocol for Epigenetic Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chromatin immunoprecipitation (ChIP) assays were performed using EZ-CHIP KIT according to the manufacturer instruction (Millipore, USA). EZH2 and SUV39H1 were obtained from Abcam. H3 trimethyl Lys 27 antibody, Histone H3 and Acetyl-Histone H3 Lys27 was from Millipore. The ChIP primer sequences were listed in Supplementary Table S1 . Quantification of immunoprecipitated DNA was performed using qPCR. ChIP data were calculated as a percentage relative to the input DNA by the equation 2[Input Ct- Target Ct] × 0.1 × 100.
4
Chromatin Modification Protein Analysis
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!