Dynasore

To detect the intracellular transport of the rGSTP1, we labeled rGSTP1 with fluorescein isothiocyanate (FITC) to treat the Hepa1-6 cells. Briefly, cells were starved for 12 h before being treated with or without inhibitors of endocytosis (chlorpromazine hydrochloride (CPZ; Targetmol, Washington, MA; 20 μM), dynasore (Targetmol; 50 μM) or MβCD (Targetmol; 0.5 mM)) for 1 h, followed by 1 h co-incubation with FITC or FITC-GSTP1 (1 μM). Images were taken under a ZEISS 780 confocal microscope (ZEISS, Oberkochen, Germany).

Ammonium chloride (NH₄Cl) and chloroquine were purchased from Sigma (St. Louis, MO, USA). Chlorpromazine (CPZ), methyl-β-cyclodextrin (MβCD), dynasore, and wortmannin were purchased from TargetMol (Boston, MA, USA). 5-Ethyl-N-isopropyl amiloride (EIPA) was purchased from MedChemExpress (Monmouth Junction, NJ, USA).
An anti-FAdV-4-fiber2 monoclonal antibody (mAb 3C2) was kindly provided by Prof. Jianqiang Ye (Yangzhou University, Yangzhou, Jiangsu, China). A rabbit polyclonal anti-FAdV-4-penton antibody was generated by our laboratory. Rabbit polyclonal anti-caveolin-1 and clathrin HC antibodies were purchased from Santa Cruz Biotechnology (CA, USA). Anti-β-actin antibodies, HRP-conjugated secondary antibodies, and FITC-conjugated anti-mouse and anti-rabbit IgG were purchased from TransGen Biotech (Beijing, China). TRITC-phalloidin and FITC-dextran were purchased from Sigma.
A pGFP-V-RS (vector), scramble shRNAs, and shRNAs targeting clathrin heavy chain (CHC) (shCHC) were kindly provided by Dr Hung-Jen Liu (Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan). siRNA targeting caveolin-1 (5′-CCACTTTCACTGTAACAAA-3′) and negative siRNA were synthesized by RiboBio (Guangzhou, China).