The morphology and structure of PCL/NS nanofibrous mesh were observed using scanning electron microscopy (SEM; Hitachi S-3400N; Hitachi Ltd., Tokyo, Japan), and the diameter of NS was measured using ImageJ software by two independent researchers (five SEM images). The chemical structure of the surface of PCL, PCL/DA and PCL/NS were characterized using a PerkinElmer Fourier transform infrared (FTIR) spectrometer (100S). The water contact angle was investigated by a contact angle analyzer (Theta Lite 101; Biolin Scientific, Stockholm, Sweden).
100 ftir spectrometer
Manufactured by PerkinElmer
Sourced in United States, United Kingdom
The 100 FTIR spectrometer is a laboratory instrument designed for conducting Fourier Transform Infrared (FTIR) spectroscopy. The core function of this product is to analyze the infrared absorption and emission characteristics of various samples, providing information about their molecular composition and structure.
Automatically generated - may contain errors
Lab products found in correlation
Theta lite 101, by Biolin Scientific (2 mentions)
Avance 500 spectrometer, by Bruker (2 mentions)
Sdt q600 instrument, by TA Instruments (2 mentions)
Avance 400 spectrometer, by Bruker (2 mentions)
Inova 400 spectrometer, by Agilent Technologies (1 mentions)
Zetasizer nano zs instrument, by Malvern Panalytical (1 mentions)
Tecnai 10 microscope, by Philips (1 mentions)
Optilab refractive index detector, by Wyatt Technology (1 mentions)
Dd2 600 mhz nmr spectrometer, by Agilent Technologies (1 mentions)
Libra 200 feg, by Zeiss (1 mentions)
Crossbeam 340, by Zeiss (1 mentions)
2400 series 2 chns o analyzer, by PerkinElmer (1 mentions)
Avance 400, by Bruker (1 mentions)
Avance 300, by Bruker (1 mentions)
Inca energy 350 x max, by Oxford Instruments (1 mentions)
Micromass quattro 2 spectrometer, by Waters Corporation (1 mentions)
Jsm 6610, by JEOL (1 mentions)
Avance 300 mhz nmr spectrometer, by Bruker (1 mentions)
Cryostream device, by Oxford Cryosystems (1 mentions)
Rf 5301pc spectro uorophotometer, by Shimadzu (1 mentions)
48 protocols using 100 ftir spectrometer
1
Characterization of PCL/NS Nanofibrous Mesh
2
Multimodal Characterization of Nanomaterials
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1H NMR spectra were recorded on a Varian INOVA-400 spectrometer operating at 400 MHz. FT-IR spectra were acquired on a Perkin-Elmer FT-IR spectrometer (100 S). Gel permeation chromatography (GPC) measurement was carried out using a Waters model 440, equipped with a Wyatt Optilab Refractive Index detector. Dynamic light scattering (DLS) and ζ-potential measurements were performed on a Malvern Zetasizer Nano ZS instrument. The freshly prepared samples were diluted according to their scattering intensities for size determination. Unless stated otherwise, measurements were implemented at 25 °C. Transmission electron microscopy (TEM) observation was carried out on a TECNAI-10 microscope (Philips, Netherland) operating at an acceleration voltage of 80 kV.
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1H NMR spectra were recorded on a Varian INOVA-400 spectrometer operating at 400 MHz. FT-IR spectra were acquired on a Perkin-Elmer FT-IR spectrometer (100 S). Gel permeation chromatography (GPC) measurement was carried out using a Waters model 440, equipped with a Wyatt Optilab Refractive Index detector. Dynamic light scattering (DLS) and ζ-potential measurements were performed on a Malvern Zetasizer Nano ZS instrument. The freshly prepared samples were diluted according to their scattering intensities for size determination. Unless stated otherwise, measurements were implemented at 25 °C. Transmission electron microscopy (TEM) observation was carried out on a TECNAI-10 microscope (Philips, Netherland) operating at an acceleration voltage of 80 kV.
3
Synthesis and Characterization of DSPE-PEG Conjugates
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DSPE-PEG conjugates based on dopamine (Dopa), serotonin (Ser), or tyramine (Tyr) were defined as DSPE-PEG-Dopa, DSPE-PEG-Ser, and DSPE-PEG-Tyr, respectively. To synthesize these conjugates by coupling reaction, 102 μmol of Dopa·HCl, Ser·HCl, or Tyr was dissolved in 3 mL of anhydrous N,N-dimethylformamide (DMF) containing 102 μmol of TEA, into which 34 μmol of DSPE-PEG-NHS was added, followed by 24 h of reaction under the protection of nitrogen at room temperature. The reaction mixture was purified by dialysis (molecular weight cutoff, 1000 Da) against deionized water for 24 h. The final product was collected by freeze-drying. An antioxidant and anti-inflammatory material (TPCD) was synthesized according to previously reported methods67 .
1H NMR spectra were conducted on a spectrometer operating at 600 MHz (DD2, Agilent). Fourier-transform infrared (FTIR) spectra were recorded on a PerkinElmer FTIR spectrometer (100 S). UV–Vis spectroscopy was recorded on a TU-1901 spectrophotometer (Beijing Purkinje General Instrument Co., Ltd., China).
1H NMR spectra were conducted on a spectrometer operating at 600 MHz (DD2, Agilent). Fourier-transform infrared (FTIR) spectra were recorded on a PerkinElmer FTIR spectrometer (100 S). UV–Vis spectroscopy was recorded on a TU-1901 spectrophotometer (Beijing Purkinje General Instrument Co., Ltd., China).
4
Synthesis and Characterization of TPCD
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A ROS-scavenging material (defined as TPCD) was synthesized by sequentially conjugating Tempol (Tpl) and 4-(hydroxymethyl)phenylboronic acid pinacol ester (PBAP) onto β-cyclodextrin (β-CD) 49 . Stearyl triphenylphosphine (TPP) (i.e., STPP) was synthesized according to the previously reported method 50 (link). Fourier transform infrared (FT-IR) spectra were recorded on a PerkinElmer FT-IR spectrometer (100S). 1H NMR spectroscopy was performed by an Agilent DD2 600 MHz NMR spectrometer.
5
FTIR Analysis of EM, EM/PD, and EM/AgNPs Films
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The FTIR spectra of the EM, EM/PD, and EM/AgNPs films were identified using a PerkinElmer FTIR spectrometer (100S).
6
Characterization of PVDF/Nanosilica Composite Films
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The morphologies of the PVDF/NS films were observed using scanning electron microscopy (SEM, Crossbeam 340, Zeiss, Germany), and the average pore size (n = 50 pores) and thickness of each film were measured using Image J software. The morphology of the synthetic NS was observed using transmission electron microscopy (TEM, Zeiss LIBRA 200 FEG, Germany) by dropping 5 μL of mixture solution onto a carbon-coated copper grid. The Fourier transform infrared (FTIR) spectra of PVDF, PVDF/NS10, PVDF/NS25 and PVDF/NS50 films were acquired using a PerkinElmer FTIR spectrometer (100S). The water contact angles were detected using a contact angle analyzer (Theta Lite 101, Biolin Scientific, Sweden).
7
FTIR Characterization of Si-PC/GR Nanocomposite
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Si-PC/GR nanocomposite membranes were dried in the vacuum drying oven at 80 °C for 4 h, and then a Fourier transform infrared (FTIR) spectrum of Si-PC/GR nanocomposite membrane was conducted on a FTIR spectrometer 100 (Perkin-Elmer Corporation, Fremont, CA, USA) in an atmospheric environment via attenuated total reflectance method (ATR). The scanning wave number was at the range of 400–4000 cm−1.
8
Determining Functional Groups via FTIR
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The FTIR analysis was conducted by using Perkin Elmer FTIR Spectrometer 100 via the KBr method to determine the functional groups in the sample.
9
Functional Group Analysis of Synthesized Samples
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In this study a fourier transform infrared (FTIR) spectroscopy measurements were performed on a Perkin Elmer FTIR spectrometer 100 over the range of 4000-500 cm -1 . KBr powderpressed pellets were used for the identification of the functional groups of the synthesized samples.
10
FTIR Analysis of Nisin-Loaded Nanocarriers
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FTIR based spectroscopic investigation was performed using a PerkinElmer 100 FTIR spectrometer (PerkinElmer, Italy). FTIR spectra of powdered nisin and polymer were recorded with KBr disc method as reported earlier (Niaz et al., 2016 (link)) with few modifications. Powder/dried samples were mixed with an appropriate amount of KBr to obtain a final concentration (1% w/w) of samples, whereas liquid nano-formulations were directly analyzed on FTIR spectrometer. FTIR transmission spectra of empty multicomponent NCS and nisin loaded NCS were recorded from 500 to 4,000 cm−1 with a resolution of 1 cm−1.
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