37 c incubator

Manufactured by Thermo Fisher Scientific

Sourced in United States, China, Austria

The 37°C incubator is a laboratory equipment designed to maintain a constant temperature of 37°C, which is the standard temperature for many biological and medical applications. This incubator provides a controlled environment for the incubation of cell cultures, microbiological samples, and other temperature-sensitive materials.

Automatically generated - may contain errors

Lab products found in correlation

35 protocols using 37 c incubator

Regulating Neuronal Apoptosis via let-7a and MKP1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wild-type PC12 cells and PC12 cells over-expressing mitogen-activated protein kinase phosphatase-1 (MKP1) were purchased from Shenbang Technology Co., Changchun, China. PC12 cells were seeded in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Gibco, Carlsbad, CA, USA) and maintained in a 37°C incubator (Thermo Fisher, Grand Island, NY, USA) with a 5% CO₂ atmosphere. The medium was replaced every 4 days.
To detect the effects of let-7a on MKP1 expression in PC12 cells, cells were assigned to control, mimic, mimic-NC (negative control), inhibitor and inhibitor-NC groups. The control group received no treatment. Mimic, mimic-NC, inhibitor and inhibitor-NC groups were transfected with let-7a mimic, let-7a mimic negative control, let-7a inhibitor and let-7a inhibitor negative control, respectively.
To detect the effects of let-7a and MKP1 on hypoxia-induced PC12 cell apoptosis, wild-type PC12 cells were divided into control, agomir-NC and let-7a groups. PC12 cells over-expressing MKP1 were divided into let-7a + MKP1, control (no treatment), agomir-NC (transfection with let-7a mimic negative control), let-7a and let-7a + MKP1 groups (transfection with let-7a mimic). All groups were incubated in the presence of 200 μM CoCl₂ (Sigma-Aldrich, St. Louis, MO, USA) for 24 hours, and were then assessed for apoptosis.

Wang Z.K., Liu F.F., Wang Y., Jiang X.M, & Yu X.F. (2016). Let-7a gene knockdown protects against cerebral ischemia/reperfusion injury. Neural Regeneration Research, 11(2), 262-269.

+ Open protocol

+ Expand

Agarose Gel Electrophoresis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Instruments used in the present study were: Horizontal agarose electrophoresis equipment (Shanghai Jingyi Organic Glass Products and Instruments Factory, Shanghai, China), centrifuge, 37°C incubator (Thermo Fisher Scientific, Waltham, MA, USA), −20°C refrigerator (Qingdao Haier Pharmaceutical Co., Ltd., Qingdao, China), DNA thermal cycler 480 (Perkin-Elmer, Norwalk, CT, USA), ABI 3730 DNA Sequencer (Perkin-Elmer Applied Biosystems, Foster City, CA, USA); PTC-200 high throughput PCR machine (MJ Research, Inc., South San Francisco, CA, USA); and ultraviolet spectrophotometer (Mettler-Toledo, Schwerzenbach, Switzerland).

Li Q., Yuan J., Wang Y, & Zhai Y. (2017). Association between the BRAF V600E mutation and ultrasound features of the thyroid in thyroid papillary carcinoma. Oncology Letters, 14(2), 1439-1444.

+ Open protocol

+ Expand

Cytotoxicity of Alcohol and Resveratrol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HL7702 cells were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences, Shanghai Institute of Cell Biology, Chinese Academy of Sciences. Cells were cultured in DMEM (Dulbecco’s modified Eagle medium) medium with 10% (v/v) foetal calf serum (GIBCO, Suzhou, China), 2.0 mM L-glutamine, 1.5 g/L NaHCO₃, 0.1 mM nonessential amino acids, 1.0 mM sodium pyruvate, 100 IU/mL penicillin, and 100 μg/mL streptomycin. Cells were maintained in a 37 °C incubator (Thermo, Mariette, OH, USA) with 5% CO₂ in humidified air.
To investigate the cytotoxicity of alcohol, RSV, or alcohol combined with RSV, HL7702 cells were first plated in 12-well plates for 24 h and then treated with blank, 10 mM alcohol, 10 μM RSV or 10 mM alcohol together with 10 μM RSV for another 24 h. The cellular morphology was observed and photographed using an inverted light microscope. We then determined the IC50 of cell viability for these treatments in the HL7702 cells. HL7702 cells were plated in 96-well plates for 24 h and then treated with a series of concentrations for alcohol with and without 10 μM RSV and a series of concentrations for RSV with and without 30 mM alcohol. The cell viability was assessed by MTT assays, and IC50 was determined.

Zhang S., Xu Y., Ye M., Ye W., Xiao J., Zhou H., Zhang W., Shu Y., Huang Y, & Chen Y. (2022). Resveratrol in Liquor Exacerbates Alcoholic Liver Injury with a Reduced Therapeutic Effect in Mice: An Unsupervised Herbal Wine Habit Is Risky. Nutrients, 14(22), 4752.

+ Open protocol

+ Expand

Electrochemical Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The equipment used in this study were the following: the 37 °C incubator from Thermo Scientific (Waltham, MA, USA); the horizontal shaker (Vortex 4 basic) from IKA (Staufen, Germany); the magnetic separator (MS-12) from Bangs laboratories (Fishers, IN, USA); and the Spectra Max M₂ micro-plate reader from Molecular Devices (Sunnyvale, CA, USA). Electrochemical measurements were performed with a PC-controlled CHI-832 electrochemical analyzer (Chenhua, Shanghai, China).

Zhang X., Wang Z., Xie H., Sun R., Cao T., Paudyal N., Fang W, & Song H. (2018). Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples. Toxins, 10(8), 317.

+ Open protocol

+ Expand

Culturing DU145 Prostate Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human PCa cell line DU145 (American Type Culture Collection, Manassas, VA, USA) was cultured in Eagle's Minimum Essential Medium (American Type Culture Collection) with 10% fetal bovine serum (FBS; American Type Culture Collection) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Inc., Waltham, MA, USA) in a 37°C incubator (Thermo Fisher Scientific, Inc.) with 95% humidity and 5% CO₂.

Zeng S., Zhu B., Zeng J., Wu W, & Jiang C. (2018). Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/β-catenin pathway. Molecular Medicine Reports, 18(6), 5572-5578.

+ Open protocol

+ Expand

Subcutaneous Tumor Growth Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cancer Hepa 1.6 cells were cultured in Opti-MEM with GlutaMAX (Gibco) containing 10% FCS (Gibco), 1% penicillin–streptomycin (Gibco), and 60 mM 2-mercaptoethanol (Sigma-Aldrich) and were maintained in a 37°C incubator (Thermo Scientific) with 5% CO₂. Cells were harvested, washed, and resuspended in PBS. 3 × 10⁶ cells were injected subcutaneously in 150 µl of PBS. Mice were monitored every day and tumor growth was measured every 2/3 days.

Perchet T., Petit M., Banchi E.G., Meunier S., Cumano A, & Golub R. (2018). The Notch Signaling Pathway Is Balancing Type 1 Innate Lymphoid Cell Immune Functions. Frontiers in Immunology, 9, 1252.

+ Open protocol

+ Expand

Colorectal Cancer Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colorectal cancer cell lines (HCT 116 and RKO) were acquired from the Cell Bank of the Shanghai Institute of Cell Biology. The HCT 116 cells were cultured in McCoy’s 5A medium (Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin mixture added to the above medium. The RKO cells were maintained in MEM with NEAA (Meilun Biotechnology, Dalian, China) supplemented with 10% FBS and 1% penicillin–streptomycin mixture. The cells were cultured in a 37 °C incubator (Thermo Fisher, Waltham, MA, USA) with 5% CO₂. The synthesis of small interfering RNA (siRNA) was entrusted to GenePharma (Shanghai, China). The cells were placed into 6-well plates (1.5 × 10⁵ cells/well), and the siNC group was transfected with the scrambled siRNA (negative control), and the siTMED1 group was transfected with the siRNA specific for TMED1 (Table S1). All siRNA transfections were performed with GP-transfect-Mate at a 75 nM final concentration following the manufacturer’s protocol. The transfected cells were collected for the following assays 48 h after transfection. Gene knockdown efficiency was determined by real-time quantitative PCR.

Guo X., Zhou W., Jin J., Lin J., Zhang W., Zhang L, & Luan X. (2024). Integrative Multi-Omics Analysis Identifies Transmembrane p24 Trafficking Protein 1 (TMED1) as a Potential Prognostic Marker in Colorectal Cancer. Biology, 13(2), 83.

+ Open protocol

+ Expand

Cell Culture Protocols for ESCC and Normal Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human ESCC cell lines KYSE-30 and KYSE-450 and the normal NIH3T3 cells were grown in RMPI 1640 medium (HyClone, Logan, UT, USA). The normal HEK-293 cell lines were maintained in MEM (HyClone, Logan, UT, USA). All culture media were supplemented with 10% FBS (Gibco, New York, NY, USA), 100 U/ml penicillin and 100 mg/ml streptomycin, and all the cell lines were cultured in a 37 °C incubator (Thermo, USA) supplied with 5% CO₂. Moreover, all the cell lines used in the study were regularly authenticated by short tandem repeat detection and tested for the presence of mycoplasma.

Che Y., Wang J., Li Y., Lu Z., Huang J., Sun S., Mao S., Lei Y., Zang R., Sun N, & He J. (2018). Cisplatin-activated PAI-1 secretion in the cancer-associated fibroblasts with paracrine effects promoting esophageal squamous cell carcinoma progression and causing chemoresistance. Cell Death & Disease, 9(7), 759.

+ Open protocol

+ Expand

Cell Line Culture Conditions for Ovarian Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

HO8910, HO8910-PM, A2780, and SKOV3 cell lines were provided by the Affiliated Hospital of Guangdong Medical University (Zhanjiang, China). A2780 was cultured in RPMI 1640 media (Gibco, China) containing 10% fetal bovine serum (FBS, Capricorn Scientific, Germany) and 1% penicillin/streptomycin (HyClone Laboratories, USA; 100 units/mL penicillin and 100 μg/mL treptomycin). HO8910, HO8910-PM, and SKOV3 cells were cultured in McCoy’s 5A medium (Boster Biological Technology Co., Wuhan, China) supplemented with 10% FBS, 100 units/mL of penicillin, and 100 μg/mL of streptomycin. Cells were grown in a 37 °C incubator (Thermo, USA) with a humidified mixture of 5% CO₂: 95% air. During the logarithmic phase, the cells were used for experiments.

Liu B., Huang X., Li Y., Liao W., Li M., Liu Y., He R., Feng D., Zhu R, & Kurihara H. (2019). JS-K, a nitric oxide donor, induces autophagy as a complementary mechanism inhibiting ovarian cancer. BMC Cancer, 19, 645.

+ Open protocol

+ Expand

In Vitro T Cell Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Stocks of OP9 and OP9-DL1 stromal cells were a gift from J. C. Zúñiga-Pflücker. All experiments were performed in Opti-MEM with GlutaMAX (Gibco) containing 10% FCS (Gibco), 1% penicillin/streptomycin (Gibco), and 60mM 2-mercaptoethanol (Sigma-Aldrich) and maintained in a 37°C incubator (Thermo Scientific) with 5% CO₂. Stromal cells were plated at 70% confluency. Before addition of lymphocytes, stromal cells were irradiated (1,500 rad) and culture media supplemented with murine IL-7 (25 ng ml⁻¹; R&D Systems) and SCF (25 ng ml⁻¹; BioLegend). Fetal liver lymphocytes were enriched for α4β7 by MACS and single-cell sorted onto 96 well plates containing stromal cells and cytokines as described above. Cultures were analyzed after 6 or 10 days of culture and only colonies with more than 10 CD45.2⁺ cells were considered.

Ishizuka I.E., Chea S., Gudjonson H., Constantinides M.G., Dinner A.R., Bendelac A, & Golub R. (2016). Single cell analysis defines the divergence between the innate lymphoid cell and lymphoid tissue inducer lineages. Nature immunology, 17(3), 269-276.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!