Propranolol

Manufactured by Selleck Chemicals

Sourced in Germany

Propranolol is a laboratory equipment product that functions as a beta-blocker. It is a synthetic compound used in various research and analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using propranolol

Compound Preparation for Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

The β-blockers were resuspended in dimethyl sulfoxide (DMSO). Stock solutions were stored at -20°C for Nebivolol (50 mM, Sigma-Aldrich, ref. #N1915) and Carvedilol (50 mM, Sigma-Aldrich, ref. #C3993) and at -80°C for Propranolol (150 mM, Selleckchem, ref. S4076). The antioxidants Mito-TEMPO (MT) and Troxerutin (TROX) were resuspended in DMSO and stock at -20°C. The solutions are diluted in culture medium ex-temporaneously for the experiments.

Rossi M., Talbot J., Piris P., Grand M.L., Montero M.P., Matteudi M., Agavnian-Couquiaud E., Appay R., Keime C., Williamson D., Buric D., Bourgarel V., Padovani L., Clifford S.C., Ayrault O., Pasquier E., André N, & Carré M. (2022). Beta-blockers disrupt mitochondrial bioenergetics and increase radiotherapy efficacy independently of beta-adrenergic receptors in medulloblastoma. eBioMedicine, 82, 104149.

+ Open protocol

+ Expand

CAR-T Cells Coculture with M2 Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 5×10⁵ THP-1 cells/well or 1×10⁶ PBMCs were seeded into the six-well plates and polarized into M2 macrophages. After thoroughly washing to remove all PMA and cytokines, the macrophages were cocultured with CAR-T cells and Raji cells. The ratio of CAR-T cells, tumor cells, and macrophages was 1:3:1. In some experiments, the cells were treated with thalidomide (1 µM), VX-765 (20 µM), Ac-YVAD-CMK (10 µM), prazosin (10 µM), propranolol (10 µM), or epinephrine (1 µM) (Selleck Chemicals).

Liu D., Xu X., Dai Y., Zhao X., Bao S., Ma W., Zha L., Liu S., Liu Y., Zheng J, & Shi M. (2021). Blockade of AIM2 inflammasome or α1-AR ameliorates IL-1β release and macrophage-mediated immunosuppression induced by CAR-T treatment. Journal for Immunotherapy of Cancer, 9(1), e001466.

+ Open protocol

+ Expand

Preparation of Chemotherapeutic Drug Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Doxorubicin and propranolol were purchased from Selleckchem. Stock solutions were prepared at 10 mM and 50 mM in dimethylsulfoxide (DMSO) and stored in aliquots at − 20 °C and − 80 °C, respectively. The chemotherapeutic drugs Vinorelbine (Vinorelbine Injection 50 mg/5 ml-Sandoz) and Docetaxel (Docetaxel Accord 160 mg/8 ml-Accord) were supplied by the Institutional Pharmacy Unit. Further dilutions were made in culture medium.

Porcelli L., Garofoli M., Di Fonte R., Fucci L., Volpicella M., Strippoli S., Guida M, & Azzariti A. (2020). The β-adrenergic receptor antagonist propranolol offsets resistance mechanisms to chemotherapeutics in diverse sarcoma subtypes: a pilot study. Scientific Reports, 10, 10465.

+ Open protocol

+ Expand

Selective Beta-Adrenergic Receptor Modulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bisoprolol and propranolol were purchased from Selleckchem (Planegg, Germany). ICI‐118,551 was obtained from Sigma‐Aldrich (St Louis, MO, USA). Dimethyl sulfoxide (DMSO), obtained from MP Biomedicals (Illkirch Graffenstaden, France), was used as the solvent for Bisoprolol and propranolol, whereas ICI‐118,551 was dissolved in sterile H₂O. Bortezomib was purchased from Selleckchem and melphalan from Sigma‐Aldrich; both were dissolved in DMSO following the manufacturer's instructions. 3‐Methyladenine (3MA), obtained from Selleckchem, was dissolved in sterile H₂O following the manufacturer's instructions.

Satilmis H., Verheye E., Vlummens P., Oudaert I., Vandewalle N., Fan R., Knight J.M., De Beule N., Ates G., Massie A., Moreaux J., Maes A., De Bruyne E., Vanderkerken K., Menu E., Sloan E.K, & De Veirman K. (2022). Targeting the β2‐adrenergic receptor increases chemosensitivity in multiple myeloma by induction of apoptosis and modulating cancer cell metabolism. The Journal of Pathology, 259(1), 69-80.

+ Open protocol

+ Expand

Isolation and Characterization of MDSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

PMN-MDSCs and M-MDSCs in the mouse spleens under the indicated conditions were FACS-sorted on the BD FACSAria. The cells were in vitro cultured in RPMI-1640 (Gibco) supplemented with 10% HI-FBS, 100 U/mL penicillin and 100 μg/mL streptomycin, and then treated with 10 μM NE or 20 μM propranolol (Selleck Chemicals) for 1 h. Total RNAs of the cells were extracted by the RNeasy Mini Kit and analyzed by the SYBR Green Real-Time PCR Kit (Thermo Fisher Scientific). Gapdh mRNA levels were utilized as the internal control.

Xu Q., Cao Y., Kong F., Liu J., Chen X., Zhao Y., Lai C.H., Zhou X., Hu H., Fu W., Chen J, & Yang J. (2024). Multiple cancer cell types release LIF and Gal3 to hijack neural signals. Cell Research, 34(5), 345-354.

+ Open protocol

+ Expand

NSCLC Cell Line Culture and Drug Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human NSCLC cell lines H522, H460, A549, and H292 were purchased from the American Type Culture Collection (ATCC). All cell lines were cultured in RPMI 1640 medium (Gibco) containing 10% fetal bovine serum (Gibco) at 37 °C in a 5% CO₂ incubator. Apatinib (Selleckchem) and anlotinib (Selleckchem) were dissolved in dimethylsulfoxide (DMSO) and stored at −80 °C (20 mM, stock solution). Propranolol (Selleckchem) was dissolved in DMSO and stored at −80 °C (50 mM, stock solution). KG501 (Selleckchem) was dissolved in DMSO and stored at −80 °C (25 mM, stock solution).

Xu Y., Wang J., Wang X., Zhou X., Tang J., Jie X., Yang X., Rao X., Xu Y., Xing B., Li Z, & Wu G. (2022). Targeting ADRB2 enhances sensitivity of non-small cell lung cancer to VEGFR2 tyrosine kinase inhibitors. Cell Death Discovery, 8, 36.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!